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BMRB: 30518

6MIF

Lim5 domain of PINCH1 protein

Authors

Qin, J., Vaynberg, J.

Assembly

LIM and senescent cell antigen-like-containing domain protein 1

Entity

1. LIM and senescent cell antigen-like-containing domain protein 1, entity 1 (polymer), 79 monomers, 8977.609 Da Detail

GSGDVCFHCN RVIEGDVVSA LNKAWCVNCF ACSTCNTKLT LKNKFVEFDM KPVCKKCYEK FPLELKKRLK KLAETLGRK

2. LIM and senescent cell antigen-like-containing domain protein 1, entity ZN (non-polymer), 65.409 × 2 Da

Total weight

9108.428 Da

Max. entity weight

8977.609 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.7 %, Completeness: 78.2 %, Completeness (bb): 82.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	78.2 % (746 of 954)	81.8 % (410 of 501)	70.3 % (260 of 370)	91.6 % (76 of 83)
Backbone	82.1 % (386 of 470)	93.8 % (150 of 160)	69.1 % (161 of 233)	97.4 % (75 of 77)
Sidechain	78.0 % (436 of 559)	76.2 % (260 of 341)	82.5 % (175 of 212)	16.7 % (1 of 6)
Aromatic	21.6 % (16 of 74)	21.6 % (8 of 37)	22.2 % (8 of 36)	0.0 % (0 of 1)
Methyl	93.8 % (75 of 80)	97.5 % (39 of 40)	90.0 % (36 of 40)

1. entity 1

GSGDVCFHCN RVIEGDVVSA LNKAWCVNCF ACSTCNTKLT LKNKFVEFDM KPVCKKCYEK FPLELKKRLK KLAETLGRK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 298 K, pH 6.5, Details 0.5 mM [U-15N] Lim5-T, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Lim5-T	[U-15N]		0.5 mM
2	NaCl	natural abundance		20 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 298 K, pH 6.5, Details 1.0 mM [U-13C; U-15N] 15N 13C Lim5-T, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	15N 13C Lim5-T	[U-13C; U-15N]		1.0 mM
4	NaCl	natural abundance		20 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

Referencing offset: 2.64 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

Referencing offset: 2.64 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

Referencing offset: 0.05 ppm, Outliers: 1 Detail

Release date

2018-09-30

Citation

Non-catalytic signaling by pseudokinase ILK for regulating cell adhesion
Vaynberg, J., Fukuda, K., Lu, F., Bialkowska, K., Chen, Y., Plow, E.F., Qin, J.
Nat. Commun. (2018), 9, 4465-4465, PubMed 30367047 , DOI 10.1038/s41467-018-06906-7 , Abstract

Related entities 1. LIM and senescent cell antigen-like-containing domain protein 1, entity 1, : 2 : 42 entities Detail

Interaction partners 1. LIM and senescent cell antigen-like-containing domain protein 1, entity 1, : 42 interactors Detail

More details for 42 interactors identified by 16 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 298 K, pH 6.5, Details 0.5 mM [U-15N] Lim5-T, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Lim5-T	[U-15N]		0.5 mM
2	NaCl	natural abundance		20 mM

2 3D HNCO