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Shuttle proteins complex
Authors
Chen, X., Walters, K.J.
Assembly
26S proteasome non-ATPase regulatory subunit 4, Ubiquilin-2
Entity
1. 26S proteasome non-ATPase regulatory subunit 4, Ubiquilin-2, entity 1 (polymer, Thiol state: not present), 111 monomers, 11819.82 Da Detail

MLGLGASDFE FGVDPSADPE LALALRVSME EQRQRQEEEA RRAAAASAAE AGIATTGTED SDDALLKMTI SQQEFGRTGL PDLSSMTEEE QIAYAMQMSL QGAEFGQAES A


2. 26S proteasome non-ATPase regulatory subunit 4, Ubiquilin-2, entity 2 (polymer, Thiol state: not present), 78 monomers, 8749.065 × 2 Da Detail

APAEPKIIKV TVKTPKEKEE FAVPENSSVQ QFKEAISKRF KSQTDQLVLI FAGKILKDQD TLIQHGIHDG LTVHLVIK


Total weight
29317.951 Da
Max. entity weight
11819.82 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 89.7 %, Completeness (bb): 86.5 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All89.7 % (1916 of 2135)98.5 % (1093 of 1110)75.6 % (625 of 827)100.0 % (198 of 198)
Backbone86.5 % (969 of 1120)100.0 % (383 of 383)72.8 % (404 of 555)100.0 % (182 of 182)
Sidechain94.3 % (1124 of 1192)97.7 % (710 of 727)88.6 % (398 of 449)100.0 % (16 of 16)
Aromatic50.0 % (50 of 100)90.0 % (45 of 50)10.0 % (5 of 50)
Methyl100.0 % (214 of 214)100.0 % (107 of 107)100.0 % (107 of 107)

1. entity 1

MLGLGASDFE FGVDPSADPE LALALRVSME EQRQRQEEEA RRAAAASAAE AGIATTGTED SDDALLKMTI SQQEFGRTGL PDLSSMTEEE QIAYAMQMSL QGAEFGQAES A

2. entity 2

APAEPKIIKV TVKTPKEKEE FAVPENSSVQ QFKEAISKRF KSQTDQLVLI FAGKILKDQD TLIQHGIHDG LTVHLVIK

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.6 mM [U-100% 15N] hRpn10 196-306, 1.38 mM UBQLN2 UBL, 20 mM NaPO4, 50 mM sodium chloride, 2 mM DTT, 0.1 % sodium azide, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
1hRpn10 196-306[U-100% 15N]0.6 mM
2UBQLN2 UBLnatural abundance1.38 mM
3NaPO4natural abundance20 mM
4sodium chloridenatural abundance50 mM
5DTTnatural abundance2 mM
6sodium azidenatural abundance0.1 %
Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.6 mM [U-100% 13C] hRpn10 196-306, 1.38 mM UBQLN2 UBL, 20 mM NaPO4, 50 mM sodium chloride, 2 mM DTT, 0.1 % sodium azide, 100% D2O


#NameIsotope labelingTypeConcentration
7hRpn10 196-306[U-100% 13C]0.6 mM
8UBQLN2 UBLnatural abundance1.38 mM
9NaPO4natural abundance20 mM
10sodium chloridenatural abundance50 mM
11DTTnatural abundance2 mM
12sodium azidenatural abundance0.1 %
Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.6 mM [U-100% 15N; U-100% 2H] hRpn10 196-306, 1.38 mM UBQLN2 UBL, 20 mM NaPO4, 50 mM sodium chloride, 2 mM DTT, 0.1 % sodium azide, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
13hRpn10 196-306[U-100% 15N; U-100% 2H]0.6 mM
14UBQLN2 UBLnatural abundance1.38 mM
15NaPO4natural abundance20 mM
16sodium chloridenatural abundance50 mM
17DTTnatural abundance2 mM
18sodium azidenatural abundance0.1 %
Sample #4

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.6 mM [U-100% 13C; U-100% 15N] UBQLN2 UBL, 0.6 mM hRpn10 196-306, 20 mM NaPO4, 50 mM sodium chloride, 2 mM DTT, 0.1 % sodium azide, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
19hRpn10 196-306natural abundance0.6 mM
20UBQLN2 UBL[U-100% 13C; U-100% 15N]0.6 mM
21NaPO4natural abundance20 mM
22sodium chloridenatural abundance50 mM
23DTTnatural abundance2 mM
24sodium azidenatural abundance0.1 %
Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.6 mM [U-100% 13C; U-100% 15N] UBQLN2 UBL, 0.6 mM hRpn10 196-306, 20 mM NaPO4, 50 mM sodium chloride, 2 mM DTT, 0.1 % sodium azide, 100% D2O


#NameIsotope labelingTypeConcentration
25hRpn10 196-306natural abundance0.6 mM
26UBQLN2 UBL[U-100% 13C; U-100% 15N]0.6 mM
27NaPO4natural abundance20 mM
28sodium chloridenatural abundance50 mM
29DTTnatural abundance2 mM
30sodium azidenatural abundance0.1 %
Sample #6

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.6 mM [U-100% 13C; U-100% 15N] UBQLN2 UBL, 20 mM NaPO4, 50 mM sodium chloride, 2 mM DTT, 0.1 % sodium azide, 100% D2O


#NameIsotope labelingTypeConcentration
31UBQLN2 UBL[U-100% 13C; U-100% 15N]0.6 mM
32NaPO4natural abundance20 mM
33sodium chloridenatural abundance50 mM
34DTTnatural abundance2 mM
35sodium azidenatural abundance0.1 %

LACS Plot; CA
Referencing offset: 0.16 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 0.16 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.07 ppm, Outliers: 4 Detail
Protein Blocks Logo
Calculated from 10 models in PDB: 6MUN, Strand ID: A, B, C Detail


Release date
2018-10-25
Citation
Structure of hRpn10 Bound to UBQLN2 UBL Illustrates Basis for Complementarity between Shuttle Factors and Substrates at the Proteasome
Chen, X., Ebelle, D.L., Wright, B.J., Sridharan, V., Hooper, E., Walters, K.J.
J. Mol. Biol. (2019), 431, 939-955, PubMed 30664872 , DOI 10.1016/j.jmb.2019.01.021 ,
Related entities 1. 26S proteasome non-ATPase regulatory subunit 4, Ubiquilin-2, entity 1, : 1 : 23 : 4 : 13 entities Detail
Related entities 2. 26S proteasome non-ATPase regulatory subunit 4, Ubiquilin-2, entity 2, : 2 : 97 entities Detail
Interaction partners 2. 26S proteasome non-ATPase regulatory subunit 4, Ubiquilin-2, entity 2, : 20 interactors Detail
Experiments performed 18 experiments Detail
Chemical shift validation 4 contents Detail
Keywords Complex, STRUCTURAL PROTEIN, proteasome, shuttle factor