Shuttle proteins complex
MLGLGASDFE FGVDPSADPE LALALRVSME EQRQRQEEEA RRAAAASAAE AGIATTGTED SDDALLKMTI SQQEFGRTGL PDLSSMTEEE QIAYAMQMSL QGAEFGQAES A
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 89.7 % (1916 of 2135) | 98.5 % (1093 of 1110) | 75.6 % (625 of 827) | 100.0 % (198 of 198) |
Backbone | 86.5 % (969 of 1120) | 100.0 % (383 of 383) | 72.8 % (404 of 555) | 100.0 % (182 of 182) |
Sidechain | 94.3 % (1124 of 1192) | 97.7 % (710 of 727) | 88.6 % (398 of 449) | 100.0 % (16 of 16) |
Aromatic | 50.0 % (50 of 100) | 90.0 % (45 of 50) | 10.0 % (5 of 50) | |
Methyl | 100.0 % (214 of 214) | 100.0 % (107 of 107) | 100.0 % (107 of 107) |
1. entity 1
MLGLGASDFE FGVDPSADPE LALALRVSME EQRQRQEEEA RRAAAASAAE AGIATTGTED SDDALLKMTI SQQEFGRTGL PDLSSMTEEE QIAYAMQMSL QGAEFGQAES A2. entity 2
APAEPKIIKV TVKTPKEKEE FAVPENSSVQ QFKEAISKRF KSQTDQLVLI FAGKILKDQD TLIQHGIHDG LTVHLVIKSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.6 mM [U-100% 15N] hRpn10 196-306, 1.38 mM UBQLN2 UBL, 20 mM NaPO4, 50 mM sodium chloride, 2 mM DTT, 0.1 % sodium azide, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | hRpn10 196-306 | [U-100% 15N] | 0.6 mM | |
2 | UBQLN2 UBL | natural abundance | 1.38 mM | |
3 | NaPO4 | natural abundance | 20 mM | |
4 | sodium chloride | natural abundance | 50 mM | |
5 | DTT | natural abundance | 2 mM | |
6 | sodium azide | natural abundance | 0.1 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.6 mM [U-100% 13C] hRpn10 196-306, 1.38 mM UBQLN2 UBL, 20 mM NaPO4, 50 mM sodium chloride, 2 mM DTT, 0.1 % sodium azide, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | hRpn10 196-306 | [U-100% 13C] | 0.6 mM | |
8 | UBQLN2 UBL | natural abundance | 1.38 mM | |
9 | NaPO4 | natural abundance | 20 mM | |
10 | sodium chloride | natural abundance | 50 mM | |
11 | DTT | natural abundance | 2 mM | |
12 | sodium azide | natural abundance | 0.1 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.6 mM [U-100% 15N; U-100% 2H] hRpn10 196-306, 1.38 mM UBQLN2 UBL, 20 mM NaPO4, 50 mM sodium chloride, 2 mM DTT, 0.1 % sodium azide, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | hRpn10 196-306 | [U-100% 15N; U-100% 2H] | 0.6 mM | |
14 | UBQLN2 UBL | natural abundance | 1.38 mM | |
15 | NaPO4 | natural abundance | 20 mM | |
16 | sodium chloride | natural abundance | 50 mM | |
17 | DTT | natural abundance | 2 mM | |
18 | sodium azide | natural abundance | 0.1 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.6 mM [U-100% 13C; U-100% 15N] UBQLN2 UBL, 0.6 mM hRpn10 196-306, 20 mM NaPO4, 50 mM sodium chloride, 2 mM DTT, 0.1 % sodium azide, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
19 | hRpn10 196-306 | natural abundance | 0.6 mM | |
20 | UBQLN2 UBL | [U-100% 13C; U-100% 15N] | 0.6 mM | |
21 | NaPO4 | natural abundance | 20 mM | |
22 | sodium chloride | natural abundance | 50 mM | |
23 | DTT | natural abundance | 2 mM | |
24 | sodium azide | natural abundance | 0.1 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.6 mM [U-100% 13C; U-100% 15N] UBQLN2 UBL, 0.6 mM hRpn10 196-306, 20 mM NaPO4, 50 mM sodium chloride, 2 mM DTT, 0.1 % sodium azide, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
25 | hRpn10 196-306 | natural abundance | 0.6 mM | |
26 | UBQLN2 UBL | [U-100% 13C; U-100% 15N] | 0.6 mM | |
27 | NaPO4 | natural abundance | 20 mM | |
28 | sodium chloride | natural abundance | 50 mM | |
29 | DTT | natural abundance | 2 mM | |
30 | sodium azide | natural abundance | 0.1 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.6 mM [U-100% 13C; U-100% 15N] UBQLN2 UBL, 20 mM NaPO4, 50 mM sodium chloride, 2 mM DTT, 0.1 % sodium azide, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
31 | UBQLN2 UBL | [U-100% 13C; U-100% 15N] | 0.6 mM | |
32 | NaPO4 | natural abundance | 20 mM | |
33 | sodium chloride | natural abundance | 50 mM | |
34 | DTT | natural abundance | 2 mM | |
35 | sodium azide | natural abundance | 0.1 % |