BMRBj - BMREntryMaster

Found 1 Document (2.639 seconds)

BMRB: 30545

6N8C

Structure of the Huttingtin tetramer/dimer mixture determined by paramagnetic NMR

Authors

Schwieters, C.D., Kotler, S.A., Schmidt, T., Ceccon, A., Ghirlando, R., Libich, D.S., Clore, G.M.

Assembly

Huntingtin

Entity

1. Huntingtin (polymer, Thiol state: not present), 23 monomers, 2740.090 × 4 Da Detail

ATLEKLMKAF ESLKSFQQQQ QQQ

Total weight

10960.36 Da

Max. entity weight

2740.09 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.3 %, Completeness: 29.5 %, Completeness (bb): 42.4 % Detail

Polymer type: polypeptide(L)

	Total	¹³C	¹⁵N
All	29.5 % (41 of 139)	22.0 % (24 of 109)	56.7 % (17 of 30)
Backbone	42.4 % (39 of 92)	31.9 % (22 of 69)	73.9 % (17 of 23)
Sidechain	4.3 % (3 of 70)	4.8 % (3 of 63)	0.0 % (0 of 7)
Aromatic	0.0 % (0 of 10)	0.0 % (0 of 10)
Methyl	0.0 % (0 of 9)	0.0 % (0 of 9)

1. entity 1

ATLEKLMKAF ESLKSFQQQQ QQQ

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 6.5, Details 1 mM [U-13C; U-15N] httNT-Q7, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	httNT-Q7	[U-13C; U-15N]		1 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 6.5, Details 1 mM 2-13C glucose; U-15N httNT-Q7, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
2	2-13C glucose, httNT-Q7	[U-15N]		1 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 6.5, Details 0.6 mM [U-15N] httNT-Q7, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	httNT-Q7	[U-15N]		0.6 mM

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 6.5, Details 0.75 mM 2-13C glucose; U-15N httNT-Q7, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
4	2-13C glucose, httNT-Q7	[U-15N]		0.75 mM

Sample #5

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 6.5, Details 0.4 mM 2-13C glucose; U-15N httNT-Q7, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
5	2-13C glucose, httNT-Q7	[U-15N]		0.4 mM

Protein Blocks Logo

Calculated from 10 models in PDB: 6N8C, Strand ID: A, B, C, D Detail

Release date

2018-12-04

Citation

Probing initial transient oligomerization events facilitating Huntingtin fibril nucleation at atomic resolution by relaxation-based NMR
Kotler, S.A., Tugarinov, V., Schmidt, T., Ceccon, A., Libich, D.S., Ghirlando, R., Schwieters, C.D., Clore, G.M.
Proc. Natl. Acad. Sci. U. S. A. (2019), 116, 3562-3571, PubMed 30808748 , DOI 10.1073/pnas.1821216116 , Abstract

Related entities 1. Huntingtin, : 1 : 14 : 1 entities Detail

Interaction partners 1. Huntingtin, : 225 interactors Detail

More details for 225 interactors identified by 25 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 K, pH 6.5, Details 1 mM [U-13C; U-15N] httNT-Q7, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	httNT-Q7	[U-13C; U-15N]		1 mM

2 2D 1H-13C CT HSQC

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 K, pH 6.5, Details 1 mM [U-13C; U-15N] httNT-Q7, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	httNT-Q7	[U-13C; U-15N]		1 mM

3 3D CBCA(CO)NH

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 6.5, Details 1 mM [U-13C; U-15N] httNT-Q7, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	httNT-Q7	[U-13C; U-15N]		1 mM

4 3D HNCACB

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 6.5, Details 1 mM [U-13C; U-15N] httNT-Q7, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	httNT-Q7	[U-13C; U-15N]		1 mM

5 3D TOCSY-HSQC

Instrument

Bruker AvanceIII - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 6.5, Details 0.6 mM [U-15N] httNT-Q7, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	httNT-Q7	[U-15N]		0.6 mM

6 13CA SQ CPMG

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 K, pH 6.5, Details 1 mM 2-13C glucose; U-15N httNT-Q7, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
2	2-13C glucose, httNT-Q7	[U-15N]		1 mM

7 13CA SQ CPMG

Instrument

Bruker AvanceIII - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 K, pH 6.5, Details 0.75 mM 2-13C glucose; U-15N httNT-Q7, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
4	2-13C glucose, httNT-Q7	[U-15N]		0.75 mM

8 15N SQ CPMG

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 K, pH 6.5, Details 1 mM 2-13C glucose; U-15N httNT-Q7, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
2	2-13C glucose, httNT-Q7	[U-15N]		1 mM

9 15N SQ CPMG

Instrument

Bruker AvanceIII - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 K, pH 6.5, Details 0.75 mM 2-13C glucose; U-15N httNT-Q7, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
4	2-13C glucose, httNT-Q7	[U-15N]		0.75 mM

10 1HN PRE

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 6.5, Details 0.6 mM [U-15N] httNT-Q7, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	httNT-Q7	[U-15N]		0.6 mM

11 13CA SQ CPMG

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 K, pH 6.5, Details 0.4 mM 2-13C glucose; U-15N httNT-Q7, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
5	2-13C glucose, httNT-Q7	[U-15N]		0.4 mM

12 15N SQ CPMG

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 K, pH 6.5, Details 0.4 mM 2-13C glucose; U-15N httNT-Q7, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
5	2-13C glucose, httNT-Q7	[U-15N]		0.4 mM

nullKeywords dimer of dimers, tetramer

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Found 1 Document (2.639 seconds)

BMRB: 30545

Sample #1

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Sample #5

Related entities 1. Huntingtin, : 1 : 14 : 1 entities Detail

Interaction partners 1. Huntingtin, : 225 interactors Detail

Experiments performed 12 experiments Detail

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