BMRB: 30546

RNA Duplex containing the internal loop 5'-GCUU/3'-UUCG

Authors

Berger, K.D., Kennedy, S.D., Turner, D.H.

Assembly

RNA (5'-R(*CP*GP*CP*AP*GP*CP*UP*UP*AP*CP*GP*C)-3'), RNA (5'-R(*GP*CP*GP*UP*GP*CP*UP*UP*UP*GP*CP*G)-3')

Entity

1. RNA (5'-R(*CP*GP*CP*AP*GP*CP*UP*UP*AP*CP*GP*C)-3') (polymer, Thiol state: not present), 12 monomers, 3850.284 Da Detail

CGCAGCUUAC GC

2. RNA (5'-R(*GP*CP*GP*UP*GP*CP*UP*UP*UP*GP*CP*G)-3') (polymer, Thiol state: not present), 12 monomers, 3884.252 Da Detail

GCGUGCUUUG CG

Total weight

7734.536 Da

Max. entity weight

3884.252 Da

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 45.0 %, Completeness (bb): 31.9 % Detail

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Polymer type: polyribonucleotide

	Total	1H	13C	31P
All	45.0 % (179 of 398)	69.2 % (148 of 214)	15.0 % (24 of 160)	29.2 % (7 of 24)
Suger, PO4	31.9 % (92 of 288)	59.0 % (85 of 144)	0.0 % (0 of 120)	29.2 % (7 of 24)
Nucleobase	79.1 % (87 of 110)	90.0 % (63 of 70)	60.0 % (24 of 40)
Aromatic	75.5 % (71 of 94)	87.0 % (47 of 54)	60.0 % (24 of 40)

1. RNA (5'-R(*CP*GP*CP*AP*GP*CP*UP*UP*AP*CP*GP*C)-3')

CGCAGCUUAC GC

2. RNA (5'-R(*GP*CP*GP*UP*GP*CP*UP*UP*UP*GP*CP*G)-3')

GCGUGCUUUG CG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.2, Details 1 mM RNA (5'-R(*CP*GP*CP*AP*GP*CP*UP*UP*AP*CP*GP*C)-3'), 1 mM RNA (5'-R(*GP*CP*GP*UP*GP*CP*UP*UP*UP*GP*CP*G)-3'), 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RNA (5'-R(*CP*GP*CP*AP*GP*CP*UP*UP*AP*CP*GP*C)-3')	none		1 mM
2	RNA (5'-R(*GP*CP*GP*UP*GP*CP*UP*UP*UP*GP*CP*G)-3')	none		1 mM
3	sodium chloride	natural abundance		80 mM
4	sodium phosphate	natural abundance		50 mM

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Release date

2018-12-12

Citation

Nuclear Magnetic Resonance Reveals That GU Base Pairs Flanking Internal Loops Can Adopt Diverse Structures
Berger, K.D., Kennedy, S.D., Turner, D.H.
Biochemistry (2019), 58, 1094-1108, PubMed 30702283 , DOI 10.1021/acs.biochem.8b01027 , Abstract

Related entities 1. RNA (5'-R(*CP*GP*CP*AP*GP*CP*UP*UP*AP*CP*GP*C)-3'), : 1 entities Detail

Related entities 2. RNA (5'-R(*GP*CP*GP*UP*GP*CP*UP*UP*UP*GP*CP*G)-3'), : 1 entities Detail

Experiments performed 3 experiments Detail