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BMRB: 30554

6NHY

Structure of the transmembrane domain of the Death Receptor 5 mutant (G217Y) - Trimer Only

Authors

Chou, J.J., Pan, L., Zhao, L., Chen, W., Piai, A., Fu, T., Wu, H., Liu, Z.

Assembly

Tumor necrosis factor receptor superfamily member 10B

Entity

1. Tumor necrosis factor receptor superfamily member 10B (polymer), 36 monomers, 3828.794 × 3 Da Detail

MPGSLSGIII YVTVAAVVLI VAVFVCKSLL WKKVLP

Total weight

11486.382 Da

Max. entity weight

3828.794 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.4 %, Completeness: 31.9 %, Completeness (bb): 64.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	31.9 % (137 of 430)	15.7 % (34 of 216)	39.1 % (70 of 179)	94.3 % (33 of 35)
Backbone	64.6 % (137 of 212)	47.2 % (34 of 72)	66.0 % (70 of 106)	97.1 % (33 of 34)
Sidechain	1.2 % (3 of 252)	0.0 % (0 of 144)	2.8 % (3 of 107)	0.0 % (0 of 1)
Aromatic	0.0 % (0 of 30)	0.0 % (0 of 15)	0.0 % (0 of 14)	0.0 % (0 of 1)
Methyl	0.0 % (0 of 76)	0.0 % (0 of 38)	0.0 % (0 of 38)

1. entity 1

MPGSLSGIII YVTVAAVVLI VAVFVCKSLL WKKVLP

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 7, Details 0.8 mM [U-13C; U-15N; 85%-2H] Transmembrane Domain of DR5 Mutant G217Y, 50 mM DMPC, 100 mM DHPC, 20 mM sodium phosphate, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	Transmembrane Domain of DR5 Mutant G217Y	[U-13C; U-15N; 85%-2H]	0.8 (±0.1) mM
2	DMPC	natural abundance	50 (±3.0) mM
3	DHPC	natural abundance	100 (±3.0) mM
4	sodium phosphate	natural abundance	20 (±2.0) mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 7, Details 0.8 mM [U-13C; U-15N] Transmembrane Domain of DR5 Mutant G217Y, 50 mM [acyl chain U-2H] DMPC, 100 mM [acyl chain U-2H] DHPC, 20 mM sodium phosphate, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
5	Transmembrane Domain of DR5 Mutant G217Y	[U-13C; U-15N]	0.8 (±0.1) mM
6	DMPC	[acyl chain U-2H]	50 (±3.0) mM
7	DHPC	[acyl chain U-2H]	100 (±3.0) mM
8	sodium phosphate	natural abundance	20 (±2.0) mM

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 7, Details 0.4 mM [15%-13C; U-15N; H-2H] Transmembrane Domain of DR5 Mutant G217Y, 50 mM [acyl chain U-2H] DMPC, 100 mM [acyl chain U-2H] DHPC, 20 mM sodium phosphate, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
9	Transmembrane Domain of DR5 Mutant G217Y	[15%-13C; U-15N; H-2H]	0.4 (±0.1) mM
10	DMPC	[acyl chain U-2H]	50 (±3.0) mM
11	DHPC	[acyl chain U-2H]	100 (±3.0) mM
12	sodium phosphate	natural abundance	20 (±2.0) mM

Protein Blocks Logo

Calculated from 15 models in PDB: 6NHY, Strand ID: A, B, C Detail

Release date

2019-02-14

Citation

Higher-Order Clustering of the Transmembrane Anchor of DR5 Drives Signaling
Pan, L., Fu, T.M., Zhao, W., Zhao, L., Chen, W., Qiu, C., Liu, W., Liu, Z., Piai, A., Fu, Q., Chen, S., Wu, H., Chou, J.J.
Cell (2019), 176, 1477-1489, PubMed 30827683 , DOI 10.1016/j.cell.2019.02.001 , Abstract

Related entities 1. Tumor necrosis factor receptor superfamily member 10B, : 1 : 3 : 5 entities Detail

Interaction partners 1. Tumor necrosis factor receptor superfamily member 10B, : 14 interactors Detail

More details for 14 interactors identified by 8 citations