BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	95.7 % (639 of 668)	95.1 % (331 of 348)	96.9 % (247 of 255)	93.8 % (61 of 65)
Backbone	96.3 % (339 of 352)	96.7 % (119 of 123)	96.5 % (165 of 171)	94.8 % (55 of 58)
Sidechain	95.4 % (352 of 369)	94.2 % (212 of 225)	97.8 % (134 of 137)	85.7 % (6 of 7)
Aromatic	100.0 % (22 of 22)	100.0 % (11 of 11)	100.0 % (10 of 10)	100.0 % (1 of 1)
Methyl	95.0 % (76 of 80)	95.0 % (38 of 40)	95.0 % (38 of 40)

1. PSHCP

SNAMELDLQP GDVVKVLESA ALGWVRARVI RVKSGGRVVV QSDQGREFTA RGNQVRLIE

Show sample condition

Sample #1

Solvent system 99% H2O, 1% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.4 mM [U-15N] PSHCP, 20 mM sodium phosphate, 200 mM sodium chloride, 0.2 mM TCEP, 99% H2O, 1% D2O

#	Name	Isotope labeling	Concentration
1	PSHCP	[U-15N]	1.4 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	TCEP	natural abundance	0.2 mM

Sample #2

Solvent system 99% H2O, 1% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.6 mM [U-15N, -13C] PSHCP, 20 mM sodium phosphate, 200 mM sodium chloride, 0.2 mM TCEP, 99% H2O, 1% D2O

#	Name	Isotope labeling	Concentration
5	PSHCP	[U-15N, -13C]	1.6 mM
6	sodium phosphate	natural abundance	20 mM
7	sodium chloride	natural abundance	200 mM
8	TCEP	natural abundance	0.2 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.24 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.24 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.39 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 6NNB, Strand ID: A Detail

Release date

2019-01-22

Citation

The structure of a highly conserved picocyanobacterial protein reveals a Tudor domain with an RNA binding function
Bauer, K.M., Dicovitsky, R., Pellegrini, M., Zhaxybayeva, O., Ragusa, M.J.
J. Biol. Chem. (2019), 294, 14333-14344, PubMed 31391250 , DOI 10.1074/jbc.RA119.007938 , Abstract

Related entities 1. PSHCP, : 1 : 35 entities Detail

Experiments performed 16 experiments Detail

1 3D_15N-separated_NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 99% H2O, 1% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.4 mM [U-15N] PSHCP, 20 mM sodium phosphate, 200 mM sodium chloride, 0.2 mM TCEP, 99% H2O, 1% D2O

#	Name	Isotope labeling	Concentration
1	PSHCP	[U-15N]	1.4 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	TCEP	natural abundance	0.2 mM

2 3D_13C-separated_NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 99% H2O, 1% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.6 mM [U-15N, -13C] PSHCP, 20 mM sodium phosphate, 200 mM sodium chloride, 0.2 mM TCEP, 99% H2O, 1% D2O

#	Name	Isotope labeling	Concentration
5	PSHCP	[U-15N, -13C]	1.6 mM
6	sodium phosphate	natural abundance	20 mM
7	sodium chloride	natural abundance	200 mM
8	TCEP	natural abundance	0.2 mM

3 2D 1H-15N HSQC

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PSHCP	[U-15N]	1.4 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	TCEP	natural abundance	0.2 mM

4 2D 1H-13C HSQC aliphatic

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
5	PSHCP	[U-15N, -13C]	1.6 mM
6	sodium phosphate	natural abundance	20 mM
7	sodium chloride	natural abundance	200 mM
8	TCEP	natural abundance	0.2 mM

5 3D HNCACB

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
5	PSHCP	[U-15N, -13C]	1.6 mM
6	sodium phosphate	natural abundance	20 mM
7	sodium chloride	natural abundance	200 mM
8	TCEP	natural abundance	0.2 mM

6 3D HNCA

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
5	PSHCP	[U-15N, -13C]	1.6 mM
6	sodium phosphate	natural abundance	20 mM
7	sodium chloride	natural abundance	200 mM
8	TCEP	natural abundance	0.2 mM

7 3D HNCO

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
5	PSHCP	[U-15N, -13C]	1.6 mM
6	sodium phosphate	natural abundance	20 mM
7	sodium chloride	natural abundance	200 mM
8	TCEP	natural abundance	0.2 mM

8 3D HN(CO)CA

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
5	PSHCP	[U-15N, -13C]	1.6 mM
6	sodium phosphate	natural abundance	20 mM
7	sodium chloride	natural abundance	200 mM
8	TCEP	natural abundance	0.2 mM

9 3D CBCA(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
5	PSHCP	[U-15N, -13C]	1.6 mM
6	sodium phosphate	natural abundance	20 mM
7	sodium chloride	natural abundance	200 mM
8	TCEP	natural abundance	0.2 mM

10 3D HBHA(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
5	PSHCP	[U-15N, -13C]	1.6 mM
6	sodium phosphate	natural abundance	20 mM
7	sodium chloride	natural abundance	200 mM
8	TCEP	natural abundance	0.2 mM

11 3D H(CCO)NH

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
5	PSHCP	[U-15N, -13C]	1.6 mM
6	sodium phosphate	natural abundance	20 mM
7	sodium chloride	natural abundance	200 mM
8	TCEP	natural abundance	0.2 mM

12 3D HCCH-COSY

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
5	PSHCP	[U-15N, -13C]	1.6 mM
6	sodium phosphate	natural abundance	20 mM
7	sodium chloride	natural abundance	200 mM
8	TCEP	natural abundance	0.2 mM

13 3D HCCH-TOCSY

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
5	PSHCP	[U-15N, -13C]	1.6 mM
6	sodium phosphate	natural abundance	20 mM
7	sodium chloride	natural abundance	200 mM
8	TCEP	natural abundance	0.2 mM

14 3D HN(CA)CO

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
5	PSHCP	[U-15N, -13C]	1.6 mM
6	sodium phosphate	natural abundance	20 mM
7	sodium chloride	natural abundance	200 mM
8	TCEP	natural abundance	0.2 mM

15 3D H(CCO)NH

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
5	PSHCP	[U-15N, -13C]	1.6 mM
6	sodium phosphate	natural abundance	20 mM
7	sodium chloride	natural abundance	200 mM
8	TCEP	natural abundance	0.2 mM

16 2D_NOESY

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PSHCP	[U-15N]	1.4 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	TCEP	natural abundance	0.2 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30559_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr30559_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	717		98.3 (chain: 1, length: 59)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 59, Sequence coverage: 98.3%
- Total number of assignments
  - ¹H chemical shifts: 409
  - ¹³C chemical shifts: 247
  - ¹⁵N chemical shifts: 61
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	348	331	95.1
¹³C chemical shifts	255	247	96.9
¹⁵N chemical shifts	72	61	84.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	123	119	96.7
¹³C chemical shifts	118	113	95.8
¹⁵N chemical shifts	58	55	94.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	225	212	94.2
¹³C chemical shifts	137	134	97.8
¹⁵N chemical shifts	14	6	42.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	41	39	95.1
¹³C chemical shifts	41	39	95.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	11	11	100.0
¹³C chemical shifts	10	10	100.0
¹⁵N chemical shifts	1	1	100.0

Keywords NMR spectroscopy, RNA BINDING PROTEIN, Tudor domains, cyanobacteria, tRNA

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Found 1 Document (0.579 seconds)

BMRB: 30559

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. PSHCP, : 1 : 35 entities Detail

Experiments performed 16 experiments Detail

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Chemical shift validation 3 contents Detail