BMRB: 30567

NMR Structure of the DNA binding domain of EhMybS3

Authors

Titaux-Delgado, G.A., del Rio-Portilla, F.

Assembly

Myb family DNA-binding protein shaqkyf family

Entity

1. Myb family DNA-binding protein shaqkyf family (polymer, Thiol state: not present), 60 monomers, 6970.036 Da Detail

GPLGSKKREV WTDAEHAKFV EGLALFHKDW KKIKEYIGTK TVVQIRSHAQ KYFLKLNKTA

Formula weight

6970.036 Da

Source organism

Entamoeba histolytica

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 83.3 %, Completeness: 74.3 %, Completeness (bb): 81.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	74.3 % (295 of 397)	74.3 % (295 of 397)
Backbone	81.3 % (100 of 123)	81.3 % (100 of 123)
Sidechain	71.2 % (195 of 274)	71.2 % (195 of 274)
Aromatic	75.6 % (31 of 41)	75.6 % (31 of 41)
Methyl	90.9 % (30 of 33)	90.9 % (30 of 33)

1. entity 1

GPLGSKKREV WTDAEHAKFV EGLALFHKDW KKIKEYIGTK TVVQIRSHAQ KYFLKLNKTA

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1.5 mM EhMybS3, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	EhMybS3	natural abundance		1.5 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 6NVZ, Strand ID: A Detail

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Release date

2019-03-20

Citation

Genome-wide and structural analysis of the Myb-SHAQKYF family in Entamoeba histolytica
Cardenas-Hernandez, H., Titaux-Delgado, G.A., Castaneda-Ortiz, E.J., Torres-Larios, A., Brieba, L.G., de Rio-portilla, F., Azuara-Liceaga, E.
Biochim. Biophys. Acta. Proteins Proteom. (2021), 1869, 140601-140601, PubMed 33422669 , DOI 10.1016/j.bbapap.2021.140601 , Abstract

Related entities 1. Myb family DNA-binding protein shaqkyf family, : 1 : 140 entities Detail

Experiments performed 2 experiments Detail

Chemical shift validation 3 contents Detail