BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	95.4 % (1362 of 1427)	94.8 % (722 of 762)	98.1 % (519 of 529)	89.0 % (121 of 136)
Backbone	98.3 % (692 of 704)	99.2 % (234 of 236)	97.7 % (346 of 354)	98.2 % (112 of 114)
Sidechain	93.7 % (786 of 839)	92.8 % (488 of 526)	99.3 % (289 of 291)	40.9 % (9 of 22)
Aromatic	100.0 % (24 of 24)	100.0 % (12 of 12)	100.0 % (12 of 12)
Methyl	98.5 % (130 of 132)	97.0 % (64 of 66)	100.0 % (66 of 66)

1. entity 1

GPMAMLARRQ RDPLQALRRR NQELKQQVDS LLSESQLKEA LEPNKRQHIY QRCIQLKQAI DENKNALQKL SKADESAPVA NYNQRKEEEH TLLDKLTQQL QGLAVTISRE NITEVGAPT

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 null, Temperature 298 K, pH 6.3, Details 0.8 mM [U-99% 13C; U-99% 15N] NPHP1 CC, 100% D2O

#	Name	Isotope labeling	Type	Concentration
1	NPHP1 CC	[U-99% 13C; U-99% 15N]		0.8 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 null, Temperature 298 K, pH 6.3, Details 0.8 mM [U-99% 13C; U-99% 15N] NPHP1CC, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
2	NPHP1CC	[U-99% 13C; U-99% 15N]		0.8 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 null, Temperature 298 K, pH 6.3, Details 0.9 mM [U-99% 15N] NPHP1CC, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	NPHP1CC	[U-99% 15N]		0.9 mM

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 null, Temperature 298 K, pH 6.3, Details 0.7 mM NPHP1CC, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
4	NPHP1CC	natural abundance		0.7 mM

Sample #5

Solvent system 100% D2O, Pressure 1 null, Temperature 298 K, pH 6.3, Details 0.7 mM NPHP1CC, 100% D2O

#	Name	Isotope labeling	Type	Concentration
5	NPHP1CC	natural abundance		0.7 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	67.84 ppm	internal	indirect	0.25
¹H	DSS	methyl protons	4.8 ppm	internal	indirect	1.0
¹⁵N	DSS	nitrogen	118.32 ppm	internal	indirect	0.1

Referencing offset: -0.13 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	67.84 ppm	internal	indirect	0.25
¹H	DSS	methyl protons	4.8 ppm	internal	indirect	1.0
¹⁵N	DSS	nitrogen	118.32 ppm	internal	indirect	0.1

Referencing offset: -0.13 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	67.84 ppm	internal	indirect	0.25
¹H	DSS	methyl protons	4.8 ppm	internal	indirect	1.0
¹⁵N	DSS	nitrogen	118.32 ppm	internal	indirect	0.1

Referencing offset: -0.01 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	67.84 ppm	internal	indirect	0.25
¹H	DSS	methyl protons	4.8 ppm	internal	indirect	1.0
¹⁵N	DSS	nitrogen	118.32 ppm	internal	indirect	0.1

Referencing offset: -0.13 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 6O1Q, Strand ID: A Detail

Release date

2019-03-06

Citation

The N-Terminal Domain of NPHP1 Folds into a Monomeric Left-Handed Antiparallel Three-Stranded Coiled Coil with Anti-apoptotic Function
Mannella, V., Quilici, G., Nigro, E.A., Lampis, M., Minici, C., Degano, M., Boletta, A., Musco, G.
ACS Chem. Biol. (2019), 14, 1845-1854, PubMed 31345020 , DOI 10.1021/acschembio.9b00582 , Abstract

Related entities 1. Nephrocystin-1, : 1 : 2 : 6 entities Detail

Interaction partners 1. Nephrocystin-1, : 12 interactors Detail

More details for 12 interactors identified by 4 citations

Experiments performed 16 experiments Detail

1 2D 1H-1H NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 null, Temperature 298 K, pH 6.3, Details 0.7 mM NPHP1CC, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
4	NPHP1CC	natural abundance		0.7 mM

2 2D 1H-1H TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 null, Temperature 298 K, pH 6.3, Details 0.7 mM NPHP1CC, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
4	NPHP1CC	natural abundance		0.7 mM

3 2D 1H-1H NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 null, Temperature 298 K, pH 6.3, Details 0.7 mM NPHP1CC, 100% D2O

#	Name	Isotope labeling	Type	Concentration
5	NPHP1CC	natural abundance		0.7 mM

4 2D 1H-1H TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 null, Temperature 298 K, pH 6.3, Details 0.7 mM NPHP1CC, 100% D2O

#	Name	Isotope labeling	Type	Concentration
5	NPHP1CC	natural abundance		0.7 mM

5 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 null, Temperature 298 K, pH 6.3, Details 0.9 mM [U-99% 15N] NPHP1CC, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	NPHP1CC	[U-99% 15N]		0.9 mM

6 3D HNHA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 null, Temperature 298 K, pH 6.3, Details 0.9 mM [U-99% 15N] NPHP1CC, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	NPHP1CC	[U-99% 15N]		0.9 mM

7 3D 1H-15N NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 null, Temperature 298 K, pH 6.3, Details 0.9 mM [U-99% 15N] NPHP1CC, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	NPHP1CC	[U-99% 15N]		0.9 mM

8 3D HNCA