BMRB: 30611

NMR solution structure of YfiD

Authors

Bowman, S.E.J., Drennan, C.L.

Assembly

Autonomous glycyl radical cofactor

Entity

1. Autonomous glycyl radical cofactor (polymer), 127 monomers, 14268.00 Da Detail

MITGIQITKA ANDDLLNSFW LLDSEKGEAR CIVAKAGFAE DEVVAVSKLG DIEYREVPVE VKPEVRVEGG QHLNVNVLRR ETLEDAVKHP EKYPQLTIRV SGYAVRFNSL TPEQQRDVIA RTFTESL

Formula weight

14268.0 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 52.0 %, Completeness: 31.8 %, Completeness (bb): 36.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	31.8 % (469 of 1474)	40.3 % (308 of 765)	17.5 % (101 of 576)	45.1 % (60 of 133)
Backbone	36.4 % (274 of 752)	47.7 % (122 of 256)	24.6 % (92 of 374)	49.2 % (60 of 122)
Sidechain	25.7 % (216 of 842)	36.0 % (183 of 509)	10.2 % (33 of 322)	0.0 % (0 of 11)
Aromatic	0.0 % (0 of 84)	0.0 % (0 of 42)	0.0 % (0 of 41)	0.0 % (0 of 1)
Methyl	21.1 % (35 of 166)	34.9 % (29 of 83)	7.2 % (6 of 83)

1. entity 1

MITGIQITKA ANDDLLNSFW LLDSEKGEAR CIVAKAGFAE DEVVAVSKLG DIEYREVPVE VKPEVRVEGG QHLNVNVLRR ETLEDAVKHP EKYPQLTIRV SGYAVRFNSL TPEQQRDVIA RTFTESL

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 760 mmHg, Temperature 293 K, pH 7.2, Details 0.7 mM [U-15N] YfiD, 18 mM HEPES, 2.7 mM ammonium sulfate, 10 % v/v D2O, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	YfiD	[U-15N]		0.7 mM
2	HEPES	natural abundance		18 mM
3	ammonium sulfate	natural abundance		2.7 mM
4	D2O	natural abundance		10 % v/v

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 760 mmHg, Temperature 293 K, pH 7.2, Details 1.3 mM [U-100% 13C; U-100% 15N] YfiD, 18 mM HEPES, 2.7 mM ammonium sulfate, 10 % v/v D2O, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
5	YfiD	[U-100% 13C; U-100% 15N]		1.3 mM
6	HEPES	natural abundance		18 mM
7	ammonium sulfate	natural abundance		2.7 mM
8	D2O	natural abundance		10 % v/v

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Protein Blocks Logo

Calculated from 10 models in PDB: 6OWR, Strand ID: A Detail

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Release date

2019-07-02

Citation

Solution structure and biochemical characterization of a spare part protein that restores activity to an oxygen-damaged glycyl radical enzyme
Bowman, S.E.J., Backman, L.R.F., Bjork, R.E., Andorfer, M.C., Yori, S., Caruso, A., Stultz, C.M., Drennan, C.L.
J. Biol. Inorg. Chem. (2019), 24, 817-829, PubMed 31250200 , DOI 10.1007/s00775-019-01681-2 , Abstract

Related entities 1. Autonomous glycyl radical cofactor, : 1 : 4 : 1 : 35 entities Detail

Interaction partners 1. Autonomous glycyl radical cofactor, : 21 interactors Detail

Experiments performed 8 experiments Detail

Chemical shift validation 3 contents Detail