BMRB: 30625

Antimicrobial lasso peptide ubonodin

Authors

Link, A.J., Cheung-Lee, W.L.

Assembly

Ubonodin

Entity

1. Ubonodin (polymer, Thiol state: not present), 28 monomers, 3217.463 Da Detail

GGDGSIAEYF NRPMHIHDWQ IMDSGYYG

Formula weight

3217.463 Da

Source organism

Burkholderia ubonensis

Exptl. method

solution NMR

Refine. method

molecular dynamicsmolecular dynamics

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.3 %, Completeness (bb): 100.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	96.3 % (158 of 164)	96.3 % (158 of 164)
Backbone	100.0 % (60 of 60)	100.0 % (60 of 60)
Sidechain	94.2 % (98 of 104)	94.2 % (98 of 104)
Aromatic	92.6 % (25 of 27)	92.6 % (25 of 27)
Methyl	100.0 % (7 of 7)	100.0 % (7 of 7)

1. entity 1

GGDGSIAEYF NRPMHIHDWQ IMDSGYYG

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 295.2 K, pH 7, Details 9 mg/mL Ubonodin, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	Ubonodin	natural abundance		9 mg/mL

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 295.2 K, pH 7, Details 4.6 mg/mL Ubonodin, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
2	Ubonodin	natural abundance		4.6 mg/mL

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Protein Blocks Logo

Calculated from 20 models in PDB: 6POR, Strand ID: A Detail

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Release date

2019-11-25

Citation

Discovery of ubonodin, an antimicrobial lasso peptide active against members of the Burkholderia cepacia complex
Cheung-Lee, W.L., Parry, M.E., Zong, C., Cartagena, A.J., Darst, S.A., Connell, N.D., Russo, R., Link, A.J.
Chembiochem. (2019), 21, 1335-1340, PubMed 31765515 , DOI 10.1002/cbic.201900707 , Abstract

Related entities 1. Ubonodin, : 1 : 11 entities Detail

Experiments performed 5 experiments Detail

Chemical shift validation 3 contents Detail