BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	88.5 % (847 of 957)	85.9 % (427 of 497)	90.4 % (340 of 376)	95.2 % (80 of 84)
Backbone	92.2 % (483 of 524)	92.6 % (176 of 190)	90.1 % (227 of 252)	97.6 % (80 of 82)
Sidechain	85.5 % (432 of 505)	81.8 % (251 of 307)	92.3 % (181 of 196)	0.0 % (0 of 2)
Aromatic	44.4 % (8 of 18)	44.4 % (4 of 9)	44.4 % (4 of 9)
Methyl	96.6 % (114 of 118)	93.2 % (55 of 59)	100.0 % (59 of 59)

1. entity 1

MSTIALALLP LGSGGSGGSG GSGRDLRAEL PLTLEEAFHG GERVVEVAGR RVSVRIPPGV REGSVIRVPG MGGQGNPPGD LLLVVRLLPH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1 mM [U-13C; U-15N] PhoA-CBD1, 20 mM potassium phosphate, 75 mM potassium chloride, 0.04 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	PhoA-CBD1	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.14 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.14 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.29 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 6PPT, Strand ID: A Detail

Release date

2019-08-06

Citation

Structural basis for client recognition and activity of Hsp40 chaperones
Jiang, Y., Rossi, P., Kalodimos, C.G.
Science (2019), 365, 1313-1319, PubMed 31604242 , DOI 10.1126/science.aax1280 , Abstract

Related entities 1. Alkaline phosphatase,Chaperone protein DnaJ 2 (E.C.3.1.3.1), : 1 : 2 : 12 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-13C HSQC

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1 mM [U-13C; U-15N] PhoA-CBD1, 20 mM potassium phosphate, 75 mM potassium chloride, 0.04 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	PhoA-CBD1	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

2 2D 1H-15N HSQC

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA-CBD1	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

3 3D HNCACB

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA-CBD1	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

4 3D 1H-15N NOESY

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA-CBD1	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

5 3D 1H-13C NOESY

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA-CBD1	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

6 3D CCH-NOESY

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA-CBD1	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

7 3D HNCO

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA-CBD1	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

8 3D CCH-TOCSY

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA-CBD1	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

9 3D HBHA(CO)NH

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA-CBD1	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30627_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr30627_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	955		97.8 (chain: 1, length: 90)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 90, Sequence coverage: 97.8%
- Total number of assignments
  - ¹H chemical shifts: 537
  - ¹³C chemical shifts: 338
  - ¹⁵N chemical shifts: 80
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	497	425	85.5
¹³C chemical shifts	376	338	89.9
¹⁵N chemical shifts	93	80	86.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	190	176	92.6
¹³C chemical shifts	180	157	87.2
¹⁵N chemical shifts	82	80	97.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	307	249	81.1
¹³C chemical shifts	196	181	92.3
¹⁵N chemical shifts	11	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	61	56	91.8
¹³C chemical shifts	61	60	98.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	9	4	44.4
¹³C chemical shifts	9	4	44.4

Keywords CHAPERONE, Client Recognition

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Found 1 Document (0.634 seconds)

BMRB: 30627

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Alkaline phosphatase,Chaperone protein DnaJ 2 (E.C.3.1.3.1), : 1 : 2 : 12 entities Detail

Experiments performed 9 experiments Detail

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Chemical shift validation 3 contents Detail