BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	85.6 % (834 of 974)	82.7 % (420 of 508)	88.0 % (337 of 383)	92.8 % (77 of 83)
Backbone	89.8 % (469 of 522)	89.9 % (170 of 189)	88.1 % (222 of 252)	95.1 % (77 of 81)
Sidechain	82.6 % (433 of 524)	78.4 % (250 of 319)	90.1 % (183 of 203)	0.0 % (0 of 2)
Aromatic	46.4 % (13 of 28)	50.0 % (7 of 14)	42.9 % (6 of 14)
Methyl	95.5 % (107 of 112)	94.6 % (53 of 56)	96.4 % (54 of 56)

1. entity 1

MLPLLFTPVT KGSGGSGGSG GSGRDLRAEL PLTLEEAFHG GERVVEVAGR RVSVRIPPGV REGSVIRVPG MGGQGNPPGD LLLVVRLLPH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1 mM [U-100% 13C; U-100% 15N] L11-K20_CBD1, 20 mM potassium phosphate, 75 mM potassium chloride, 0.04 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	L11-K20_CBD1	[U-100% 13C; U-100% 15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.2 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.2 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.18 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 6PQ2, Strand ID: A Detail

Release date

2019-08-06

Citation

Structural basis for client recognition and activity of Hsp40 chaperones
Jiang, Y., Rossi, P., Kalodimos, C.G.
Science (2019), 365, 1313-1319, PubMed 31604242 , DOI 10.1126/science.aax1280 , Abstract

Related entities 1. Alkaline phosphatase,Chaperone DnaJ domain-containing protein fusion, : 1 : 1 : 12 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1 mM [U-100% 13C; U-100% 15N] L11-K20_CBD1, 20 mM potassium phosphate, 75 mM potassium chloride, 0.04 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	L11-K20_CBD1	[U-100% 13C; U-100% 15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

2 3D C(CO)NH TOCSY

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	L11-K20_CBD1	[U-100% 13C; U-100% 15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

3 3D CCH-TOCSY

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	L11-K20_CBD1	[U-100% 13C; U-100% 15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

4 3D HNCACB

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	L11-K20_CBD1	[U-100% 13C; U-100% 15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

5 3D CCH-NOESY

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	L11-K20_CBD1	[U-100% 13C; U-100% 15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

6 3D HNCO

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	L11-K20_CBD1	[U-100% 13C; U-100% 15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

7 3D HBHA(CO)NH

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	L11-K20_CBD1	[U-100% 13C; U-100% 15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

8 3D 1H-15N NOESY

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	L11-K20_CBD1	[U-100% 13C; U-100% 15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

9 3D 1H-13C NOESY

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	L11-K20_CBD1	[U-100% 13C; U-100% 15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

10 2D 1H-13C HSQC

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	L11-K20_CBD1	[U-100% 13C; U-100% 15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30628_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr30628_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	939		97.8 (chain: 1, length: 90)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 90, Sequence coverage: 97.8%
- Total number of assignments
  - ¹H chemical shifts: 525
  - ¹³C chemical shifts: 337
  - ¹⁵N chemical shifts: 77
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	508	417	82.1
¹³C chemical shifts	383	337	88.0
¹⁵N chemical shifts	92	77	83.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	189	171	90.5
¹³C chemical shifts	180	153	85.0
¹⁵N chemical shifts	81	77	95.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	319	246	77.1
¹³C chemical shifts	203	184	90.6
¹⁵N chemical shifts	11	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	58	54	93.1
¹³C chemical shifts	58	55	94.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	14	7	50.0
¹³C chemical shifts	14	6	42.9

Keywords CHAPERONE, Client Recognition

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Found 1 Document (0.663 seconds)

BMRB: 30628

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Alkaline phosphatase,Chaperone DnaJ domain-containing protein fusion, : 1 : 1 : 12 entities Detail

Experiments performed 10 experiments Detail

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Chemical shift validation 3 contents Detail