BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	82.5 % (781 of 947)	80.5 % (396 of 492)	85.9 % (317 of 369)	79.1 % (68 of 86)
Backbone	79.8 % (415 of 520)	76.7 % (145 of 189)	82.7 % (206 of 249)	78.0 % (64 of 82)
Sidechain	86.7 % (432 of 498)	82.8 % (251 of 303)	92.7 % (177 of 191)	100.0 % (4 of 4)
Aromatic	94.7 % (36 of 38)	94.7 % (18 of 19)	94.4 % (17 of 18)	100.0 % (1 of 1)
Methyl	91.8 % (90 of 98)	91.8 % (45 of 49)	91.8 % (45 of 49)

1. entity 1

METATAGEWQ GKGSGGSGGS GGSQDLYATL DVPAPIAVVG GKVRAMTLEG PVEVAVPPRT QAGRKLRLKG KGFPGPAGRG DLYLEVRIT

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1 mM [U-13C; U-15N] PhoA_235-245_DnaJ_191-256, 20 mM potassium phosphate, 75 mM potassium chloride, 0.04 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	PhoA_235-245_DnaJ_191-256	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.55 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 6PQE, Strand ID: A Detail

Release date

2019-09-05

Citation

Structural basis for client recognition and activity of Hsp40 chaperones
Jiang, Y., Rossi, P., Kalodimos, C.G.
Science (2019), 365, 1313-1319, PubMed 31604242 , DOI 10.1126/science.aax1280 , Abstract

Related entities 1. Alkaline phosphatase,Chaperone protein DnaJ 2 (E.C.3.1.3.1), : 1 : 1 : 11 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-13C HSQC

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1 mM [U-13C; U-15N] PhoA_235-245_DnaJ_191-256, 20 mM potassium phosphate, 75 mM potassium chloride, 0.04 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	PhoA_235-245_DnaJ_191-256	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

2 3D HNCO

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_235-245_DnaJ_191-256	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

3 3D HBHA(CO)NH

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_235-245_DnaJ_191-256	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

4 3D CCH-NOESY

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_235-245_DnaJ_191-256	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

5 3D CCH_TOCSY

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_235-245_DnaJ_191-256	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

6 3D H(CCO)NH

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_235-245_DnaJ_191-256	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

7 3D HNCACB

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_235-245_DnaJ_191-256	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

8 3D 1H-15N NOESY

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_235-245_DnaJ_191-256	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

9 3D 1H-13C NOESY

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_235-245_DnaJ_191-256	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

10 2D 1H-15N HSQC

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_235-245_DnaJ_191-256	[U-13C; U-15N]	1 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	75 mM
4	sodium azide	natural abundance	0.04 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30629_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr30629_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	850		87.6 (chain: 1, length: 89)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 89, Sequence coverage: 87.6%
- Total number of assignments
  - ¹H chemical shifts: 477
  - ¹³C chemical shifts: 308
  - ¹⁵N chemical shifts: 65
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	492	385	78.3
¹³C chemical shifts	369	308	83.5
¹⁵N chemical shifts	92	65	70.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	189	140	74.1
¹³C chemical shifts	178	133	74.7
¹⁵N chemical shifts	82	61	74.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	303	245	80.9
¹³C chemical shifts	191	175	91.6
¹⁵N chemical shifts	10	4	40.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	51	46	90.2
¹³C chemical shifts	51	46	90.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	19	18	94.7
¹³C chemical shifts	18	17	94.4
¹⁵N chemical shifts	1	1	100.0

Keywords CHAPERONE, Client Recognition

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.624 seconds)

BMRB: 30629

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Alkaline phosphatase,Chaperone protein DnaJ 2 (E.C.3.1.3.1), : 1 : 1 : 11 entities Detail

Experiments performed 10 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail