BMRB: 30630

Solution structure of OlvA(BCS)

Authors

Acedo, J.Z., van der Donk, W.A.

Assembly

OlvA(BCS)

Entity

1. OlvA(BCS) (polymer, Thiol state: all other bound), 19 monomers, 1615.832 Da Detail

ACGXGXGCAK XCAASCAAS

Formula weight

1615.832 Da

Source organism

Streptomyces olivaceus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 84.2 %, Completeness: 95.1 %, Completeness (bb): 91.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	95.1 % (58 of 61)	95.1 % (58 of 61)
Backbone	91.4 % (32 of 35)	91.4 % (32 of 35)
Sidechain	100.0 % (26 of 26)	100.0 % (26 of 26)
Methyl	100.0 % (6 of 6)	100.0 % (6 of 6)

1. entity 1

ACGXGXGCAK XCAASCAAS

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 760 mmHg, Temperature 288 K, pH 5.8, Details 2.5 mM NA OlvA(BCS), 30 mM NA sodium phosphate, 0.001 % NA DSS, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	OlvA(BCS)	natural abundance		2.5 mM
2	sodium phosphate	natural abundance		30 mM
3	DSS	natural abundance		0.001 %

Sample #2

Solvent system 100% D2O, Pressure 760 mmHg, Temperature 288 K, pH 5.8, Details 2.5 mM NA OlvA(BCS), 30 mM NA sodium phosphate, 0.001 % NA DSS, 100% D2O

#	Name	Isotope labeling	Type	Concentration
4	OlvA(BCS)	natural abundance		2.5 mM
5	sodium phosphate	natural abundance		30 mM
6	DSS	natural abundance		0.001 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 6PQF, Strand ID: A Detail

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Release date

2019-07-23

Citation

O-Methyltransferase-Mediated Incorporation of a β-Amino Acid in Lanthipeptides
Acedo, J.Z., Bothwell, I.R., An, L., Trouth, A., Frazier, C., van der Donk, W.A.
J. Am. Chem. Soc. (2019), 141, 16790-16801, PubMed 31568727 , DOI 10.1021/jacs.9b07396 , Abstract

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail