Structural Basis for Client Recognition and Activity of Hsp40 Chaperones
MKDGAVMVMS YGNSEEDGSG GSGGSGGSQD LYATLDVPAP IAVVGGKVRA MTLEGPVEVA VPPRTQAGRK LRLKGKGFPG PAGRGDLYLE VRIT
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 87.3 % (869 of 995) | 82.4 % (426 of 517) | 93.0 % (361 of 388) | 91.1 % (82 of 90) |
Backbone | 90.2 % (496 of 550) | 87.4 % (174 of 199) | 90.9 % (240 of 264) | 94.3 % (82 of 87) |
Sidechain | 86.2 % (449 of 521) | 79.2 % (252 of 318) | 98.5 % (197 of 200) | 0.0 % (0 of 3) |
Aromatic | 94.1 % (32 of 34) | 94.1 % (16 of 17) | 94.1 % (16 of 17) | |
Methyl | 99.0 % (99 of 100) | 98.0 % (49 of 50) | 100.0 % (50 of 50) |
1. entity 1
MKDGAVMVMS YGNSEEDGSG GSGGSGGSQD LYATLDVPAP IAVVGGKVRA MTLEGPVEVA VPPRTQAGRK LRLKGKGFPG PAGRGDLYLE VRITSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 1 mM [U-100% 13C; U-100% 15N] PhoA-Hsp40/DnaJ CBD2 fusion, 75 mM potassium chloride, 20 mM potassium phosphate, 0.04 % sodium azide, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | PhoA-Hsp40/DnaJ CBD2 fusion | [U-100% 13C; U-100% 15N] | 1 mM | |
2 | potassium chloride | natural abundance | 75 mM | |
3 | potassium phosphate | natural abundance | 20 mM | |
4 | sodium azide | natural abundance | 0.04 % |