BMRBj - BMREntryMaster

Found 1 Document (5.306 seconds)

BMRB: 30638

5JTL

Structural Basis for Client Recognition and Activity of Hsp40 Chaperones

Authors

Jiang, Y., Rossi, P., Kalodimos, C.G.

Assembly

Alkaline phosphatase,Chaperone protein DnaJ 2 (E.C.3.1.3.1)

Entity

1. Alkaline phosphatase,Chaperone protein DnaJ 2 (E.C.3.1.3.1), entity 1 (polymer, Thiol state: not present), 280 monomers, 30978.01 × 2 Da Detail

MAAKKDYYAI LGVPRNATQE EIKRAYKRLA RQYHPDVNKS PEAEEKFKEI NEAYAVLSDP EKRRIYDTYG TTEAPPPPPP GGYDFSGFDV EDFSEFFQEL FGPGLFGGFG RRSRKGRDLR AELPLTLEEA FHGGERVVEV AGRRVSVRIP PGVREGSVIR VPGMGGQGNP PGDLLLVVRL LPHPVFRLEG QDLYATLDVP APIAVVGGKV RAMTLEGPVE VAVPPRTQAG RKLRLKGKGF PGPAGRGDLY LEVRITIPER LTPEEEALWK KLAEAYYARA

2. Alkaline phosphatase,Chaperone protein DnaJ 2 (E.C.3.1.3.1), entity 2 (polymer), 471 monomers, 49437.97 Da Detail

MKQSTIALAL LPLLFTPVTK ARTPEMPVLE NRAAQGDITA PGGARRLTGD QTAALRDSLS DKPAKNIILL IGDGMGDSEI TAARNYAEGA GGFFKGIDAL PLTGQYTHYA LNKKTGKPDY VTDSAASATA WSTGVKTYNG ALGVDIHEKD HPTILEMAKA AGLATGNVST AELQDATPAA LVAHVTSRKC YGPSATSEKC PGNALEKGGK GSITEQLLNA RADVTLGGGA KTFAETATAG EWQGKTLREQ AQARGYQLVS DAASLNSVTE ANQQKPLLGL FADGNMPVRW LGPKATYHGN IDKPAVTCTP NPQRNDSVPT LAQMTDKAIE LLSKNEKGFF LQVEGASIDK QDHAANPCGQ IGETVDLDEA VQRALEFAKK EGNTLVIVTA DHAHASQIVA PDTKAPGLTQ ALNTKDGAVM VMSYGNSEED SQEHTGSQLR IAAYGPHAAN VVGLTDQTDL FYTMKAALGL K

Total weight

111393.984 Da

Max. entity weight

49437.97 Da

Source organism

Thermus thermophilus HB8 , Escherichia coli

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 44.1 %, Completeness: 28.2 %, Completeness (bb): 35.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	28.2 % (2360 of 8377)	24.6 % (1069 of 4345)	30.2 % (990 of 3275)	39.8 % (301 of 757)
Backbone	35.2 % (1550 of 4404)	33.3 % (509 of 1527)	34.2 % (745 of 2177)	42.3 % (296 of 700)
Sidechain	23.2 % (1077 of 4648)	19.9 % (560 of 2818)	28.9 % (512 of 1773)	8.8 % (5 of 57)
Aromatic	5.5 % (27 of 494)	6.9 % (17 of 247)	3.7 % (9 of 243)	25.0 % (1 of 4)
Methyl	36.6 % (314 of 858)	36.6 % (157 of 429)	36.6 % (157 of 429)

1. entity 1

2. entity 2

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 25 K, pH 7, Details 1 mM [U-100% 13C; U-100% 15N; U-80% 2H] PhoA_DnaJ, 75 mM potassium chloride, 20 mM potassium phosphate, 0.04 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	PhoA_DnaJ	[U-100% 13C; U-100% 15N; U-80% 2H]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 6PSI, Strand ID: A, B, C Detail

Release date

2019-09-05

Citation

Structural basis for client recognition and activity of Hsp40 chaperones
Jiang, Y., Rossi, P., Kalodimos, C.G.
Science (2019), 365, 1313-1319, PubMed 31604242 , DOI 10.1126/science.aax1280 , Abstract

Related entities 1. Alkaline phosphatase,Chaperone protein DnaJ 2 (E.C.3.1.3.1), entity 1, : 99 entities Detail

Related entities 2. Alkaline phosphatase,Chaperone protein DnaJ 2 (E.C.3.1.3.1), entity 2, : 1 : 3 : 27 : 65 entities Detail

Interaction partners 2. Alkaline phosphatase,Chaperone protein DnaJ 2 (E.C.3.1.3.1), entity 2, : 8 interactors Detail

More details for 8 interactors identified by 5 citations

Experiments performed 12 experiments Detail

1 2D 1H-13C HSQC

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 25 K, pH 7, Details 1 mM [U-100% 13C; U-100% 15N; U-80% 2H] PhoA_DnaJ, 75 mM potassium chloride, 20 mM potassium phosphate, 0.04 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	PhoA_DnaJ	[U-100% 13C; U-100% 15N; U-80% 2H]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

2 2D 1H-15N HSQC

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_DnaJ	[U-100% 13C; U-100% 15N; U-80% 2H]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

3 2D 1H-13C HMQC

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_DnaJ	[U-100% 13C; U-100% 15N; U-80% 2H]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

4 3D 1H-13C NOESY

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_DnaJ	[U-100% 13C; U-100% 15N; U-80% 2H]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

5 3D 1H-15N NOESY

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_DnaJ	[U-100% 13C; U-100% 15N; U-80% 2H]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

6 3D CNH-sofast hmqc-NOESY-hmqc

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_DnaJ	[U-100% 13C; U-100% 15N; U-80% 2H]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

7 3D HNH-sofastNOESY-hmqc

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_DnaJ	[U-100% 13C; U-100% 15N; U-80% 2H]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

8 3D HCH-sofastNOESY-hmqc

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_DnaJ	[U-100% 13C; U-100% 15N; U-80% 2H]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

9 3D NCH-sofast hmqc-NOESY-hmqc

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_DnaJ	[U-100% 13C; U-100% 15N; U-80% 2H]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

10 3D CCH-sofast hmqc-NOESY-hmqc

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_DnaJ	[U-100% 13C; U-100% 15N; U-80% 2H]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

11 3D HNCO

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_DnaJ	[U-100% 13C; U-100% 15N; U-80% 2H]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

12 3D HNCACB

Instrument

Bruker AVANCE NEO - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	PhoA_DnaJ	[U-100% 13C; U-100% 15N; U-80% 2H]	1 mM
2	potassium chloride	natural abundance	75 mM
3	potassium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	0.04 %

Chemical shift validation 4 contents Detail

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Keywords CHAPERONE, Client Recognition

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30638_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Error

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Found 1 Document (5.306 seconds)

BMRB: 30638

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Alkaline phosphatase,Chaperone protein DnaJ 2 (E.C.3.1.3.1), entity 1, : 99 entities Detail

Related entities 2. Alkaline phosphatase,Chaperone protein DnaJ 2 (E.C.3.1.3.1), entity 2, : 1 : 3 : 27 : 65 entities Detail

Interaction partners 2. Alkaline phosphatase,Chaperone protein DnaJ 2 (E.C.3.1.3.1), entity 2, : 8 interactors Detail

Experiments performed 12 experiments Detail

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Chemical shift validation 4 contents Detail