BMRBj - BMREntryMaster

Found 1 Document (1.444 seconds)

BMRB: 30652

6Q2I

Human CstF-64 RRM

Authors

Latham, M.P., Masoumzadeh, E.

Assembly

Cleavage stimulation factor subunit 2

Entity

1. Cleavage stimulation factor subunit 2 (polymer), 108 monomers, 11950.22 Da Detail

MAGLTVRDPA VDRSLRSVFV GNIPYEATEE QLKDIFSEVG PVVSFRLVYD RETGKPKGYG FCEYQDQETA LSAMRNLNGR EFSGRALRVD NAASEKNKEE LKSLGTGA

Formula weight

11950.22 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.1 %, Completeness: 38.1 %, Completeness (bb): 70.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	38.1 % (470 of 1233)	18.6 % (119 of 639)	52.4 % (248 of 473)	85.1 % (103 of 121)
Backbone	70.5 % (451 of 640)	47.3 % (105 of 222)	77.4 % (243 of 314)	99.0 % (103 of 104)
Sidechain	14.2 % (98 of 691)	3.4 % (14 of 417)	32.7 % (84 of 257)	0.0 % (0 of 17)
Aromatic	4.9 % (4 of 82)	9.8 % (4 of 41)	0.0 % (0 of 41)
Methyl	13.9 % (15 of 108)	7.4 % (4 of 54)	20.4 % (11 of 54)

1. entity 1

MAGLTVRDPA VDRSLRSVFV GNIPYEATEE QLKDIFSEVG PVVSFRLVYD RETGKPKGYG FCEYQDQETA LSAMRNLNGR EFSGRALRVD NAASEKNKEE LKSLGTGA

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6, Details 980 uM [U-100% 13C; U-100% 15N] RNA Recognition Motif, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RNA Recognition Motif	[U-100% 13C; U-100% 15N]		980 uM

Release date

2019-08-29

Citation

A missense mutation in the CSTF2 gene that impairs the function of the RNA recognition motif and causes defects in 3' end processing is associated with intellectual disability in humans
Grozdanov, P.N., Masoumzadeh, E., Kalscheuer, V.M., Bienvenu, T., Billuart, P., Delrue, M.A., Latham, M.P., MacDonald, C.C.
Nucleic Acids Res. (2020), 48, 9804-9821, PubMed 32816001 , DOI 10.1093/nar/gkaa689 , Abstract

Related entities 1. Cleavage stimulation factor subunit 2, : 1 : 4 : 6 : 98 entities Detail

Interaction partners 1. Cleavage stimulation factor subunit 2, : 38 interactors Detail

More details for 38 interactors identified by 14 citations

Experiments performed 5 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30652_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr30652_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	444		97.2 (chain: 1, length: 108)
1	Chemical shift references	chem_shift_reference_1	Warning	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 108, Sequence coverage: 97.2%
- Total number of assignments
  - ¹³C chemical shifts: 241
  - ¹⁵N chemical shifts: 102
  - ¹H chemical shifts: 101
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	473	241	51.0
¹⁵N chemical shifts	121	102	84.3
¹H chemical shifts	639	101	15.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	216	162	75.0
¹⁵N chemical shifts	104	102	98.1
¹H chemical shifts	222	101	45.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	257	79	30.7
¹⁵N chemical shifts	17	0	0.0
¹H chemical shifts	417	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	56	7	12.5
¹H chemical shifts	56	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	41	0	0.0
¹H chemical shifts	41	0	0.0

Keywords RNA BINDING PROTEIN, RNA cleavage and polyadenylation, RNA recognition motif

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Found 1 Document (1.444 seconds)

BMRB: 30652

Sample

Related entities 1. Cleavage stimulation factor subunit 2, : 1 : 4 : 6 : 98 entities Detail

Interaction partners 1. Cleavage stimulation factor subunit 2, : 38 interactors Detail

Experiments performed 5 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail