BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	35.5 % (446 of 1255)	17.2 % (112 of 650)	48.2 % (233 of 483)	82.8 % (101 of 122)
Backbone	66.5 % (432 of 650)	45.3 % (102 of 225)	71.6 % (229 of 320)	96.2 % (101 of 105)
Sidechain	11.6 % (82 of 705)	2.1 % (9 of 425)	27.8 % (73 of 263)	0.0 % (0 of 17)
Aromatic	0.0 % (0 of 82)	0.0 % (0 of 41)	0.0 % (0 of 41)
Methyl	7.0 % (8 of 114)	5.3 % (3 of 57)	8.8 % (5 of 57)

1. entity 1

MAGLTVRDPA VDRSLRSVFV GNIPYEATEE QLKDIFSEVG PVVSFRLVYA RETGKPKGYG FCEYQDQETA LSAMRNLNGR EFSGRALRVD NAASEKNKEE LKSLGTGAPV

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6, Details 1.2 mM [U-100% 13C; U-100% 15N] CstF-64 RNA recognition motif mutant, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	CstF-64 RNA recognition motif mutant	[U-100% 13C; U-100% 15N]		1.2 mM

Protein Blocks Logo

Calculated from 10 models in PDB: 6TZE, Strand ID: A Detail

Release date

2019-08-29

Citation

A missense mutation in the CSTF2 gene that impairs the function of the RNA recognition motif and causes defects in 3' end processing is associated with intellectual disability in humans
Grozdanov, P.N., Masoumzadeh, E., Kalscheuer, V.M., Bienvenu, T., Billuart, P., Delrue, M.A., Latham, M.P., MacDonald, C.C.
Nucleic Acids Res. (2020), 48, 9804-9821, PubMed 32816001 , DOI 10.1093/nar/gkaa689 , Abstract

Related entities 1. Cleavage stimulation factor subunit 2, : 1 : 9 : 82 entities Detail

Interaction partners 1. Cleavage stimulation factor subunit 2, : 38 interactors Detail

More details for 38 interactors identified by 14 citations

Experiments performed 5 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30653_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr30653_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	423		90.9 (chain: 1, length: 110)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 110, Sequence coverage: 90.9%
- Total number of assignments
  - ¹H chemical shifts: 100
  - ¹³C chemical shifts: 223
  - ¹⁵N chemical shifts: 100
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	650	100	15.4
¹³C chemical shifts	483	223	46.2
¹⁵N chemical shifts	122	100	82.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	225	100	44.4
¹³C chemical shifts	220	157	71.4
¹⁵N chemical shifts	105	100	95.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	425	0	0.0
¹³C chemical shifts	263	66	25.1
¹⁵N chemical shifts	17	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	59	0	0.0
¹³C chemical shifts	59	3	5.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	41	0	0.0
¹³C chemical shifts	41	0	0.0

Keywords RNA BINDING PROTEIN, RNA cleavage and polyadenylation, RNA recognition motif

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Found 1 Document (1.323 seconds)

BMRB: 30653

Sample

Related entities 1. Cleavage stimulation factor subunit 2, : 1 : 9 : 82 entities Detail

Interaction partners 1. Cleavage stimulation factor subunit 2, : 38 interactors Detail

Experiments performed 5 experiments Detail

Instrument

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Chemical shift validation 3 contents Detail