Solution NMR structure of the DNAJB6b deltaST variant (Aligned on the CTD domain)
GMVDYYEVLG VQRHASPEDI KKAYRKLALK WHPDKNPENK EEAERKFKQV AEAYEVLSDA KKRDIYDKYG KEGLNGGGGG GSHFDSPFEF GFTFRNPDDV FREFFGGRDP FSFDFFEDPF EDFFGNRRGP RGGMGNFKSI STSTKMVNGR KITTKRIVEN GQERVEVEED GQLKSLTING KEQLLRLDNK
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 48.8 % (1111 of 2276) | 31.0 % (373 of 1205) | 65.3 % (570 of 873) | 84.8 % (168 of 198) |
Backbone | 77.3 % (869 of 1124) | 52.3 % (206 of 394) | 91.1 % (499 of 548) | 90.1 % (164 of 182) |
Sidechain | 30.5 % (402 of 1320) | 20.6 % (167 of 811) | 47.1 % (232 of 493) | 18.8 % (3 of 16) |
Aromatic | 3.3 % (8 of 242) | 5.0 % (6 of 121) | 0.8 % (1 of 120) | 100.0 % (1 of 1) |
Methyl | 76.2 % (99 of 130) | 72.3 % (47 of 65) | 80.0 % (52 of 65) |
1. entity 1
GMVDYYEVLG VQRHASPEDI KKAYRKLALK WHPDKNPENK EEAERKFKQV AEAYEVLSDA KKRDIYDKYG KEGLNGGGGG GSHFDSPFEF GFTFRNPDDV FREFFGGRDP FSFDFFEDPF EDFFGNRRGP RGGMGNFKSI STSTKMVNGR KITTKRIVEN GQERVEVEED GQLKSLTING KEQLLRLDNKSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.7, Details 2e-04 M [U-13C; U-15N; U-2H] DNAJB6b deltaST variant. This variant is missing residues 132-183 in comparison to the full-length DNAJB6b, 0.05 M sodium chloride, 0.02 % w/v sodium azide, 0.02 M sodium phosphate, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DNAJB6b deltaST variant | [U-13C; U-15N; U-2H] | 2e-04 M | |
2 | sodium chloride | natural abundance | 0.05 M | |
3 | sodium azide | natural abundance | 0.02 % w/v | |
4 | sodium phosphate | natural abundance | 0.02 M |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.7, Details 2e-04 M [ILV-CH3; U-13C; U-15N; U-2H] DNAJB6b deltaST variant. This variant is missing residues 132-183 in comparison to the full-length DNAJB6b, 0.05 M sodium chloride, 0.02 % w/v sodium azide, 0.02 M sodium phosphate, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | DNAJB6b deltaST variant | [ILV-CH3; U-13C; U-15N; U-2H] | 2e-04 M | |
6 | sodium chloride | natural abundance | 0.05 M | |
7 | sodium azide | natural abundance | 0.02 % w/v | |
8 | sodium phosphate | natural abundance | 0.02 M |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.7, Details 2e-04 M [ILV-CH3; U-15N; U-2H] DNAJB6b deltaST variant. This variant is missing residues 132-183 in comparison to the full-length DNAJB6b, 0.05 M sodium chloride, 0.02 % w/v sodium azide, 0.02 M sodium phosphate, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | DNAJB6b deltaST variant | [ILV-CH3; U-15N; U-2H] | 2e-04 M | |
10 | sodium chloride | natural abundance | 0.05 M | |
11 | sodium azide | natural abundance | 0.02 % w/v | |
12 | sodium phosphate | natural abundance | 0.02 M |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.7, Details 2e-04 M [U-13C; U-15N] DNAJB6b deltaST variant. This variant is missing residues 132-183 in comparison to the full-length DNAJB6b, 0.05 M sodium chloride, 0.02 % w/v sodium azide, 0.02 M sodium phosphate, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | DNAJB6b deltaST variant | [U-13C; U-15N] | 2e-04 M | |
14 | sodium chloride | natural abundance | 0.05 M | |
15 | sodium azide | natural abundance | 0.02 % w/v | |
16 | sodium phosphate | natural abundance | 0.02 M |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.7, Details 2e-04 M [U-15N;U-2H] DNAJB6b deltaST variant. This variant is missing residues 132-183 in comparison to the full-length DNAJB6b, 0.05 M sodium chloride, 0.02 % w/v sodium azide, 0.02 M sodium phosphate, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
17 | DNAJB6b deltaST variant | [U-15N;U-2H] | 2e-04 M | |
18 | sodium chloride | natural abundance | 0.05 M | |
19 | sodium azide | natural abundance | 0.02 % w/v | |
20 | sodium phosphate | natural abundance | 0.02 M |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.7, Details 2e-04 M [U-15N;U-2H] DNAJB6b deltaST variant. This variant is missing residues 132-183 in comparison to the full-length DNAJB6b, 0.1 M sodium chloride, 0.02 % w/v sodium azide, 0.02 M sodium phosphate, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
21 | DNAJB6b deltaST variant | [U-15N;U-2H] | 2e-04 M | |
22 | sodium chloride | natural abundance | 0.1 M | |
23 | sodium azide | natural abundance | 0.02 % w/v | |
24 | sodium phosphate | natural abundance | 0.02 M |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.7, Details 0.0002 M [U-15N;U-2H] DNAJB6b deltaST variant. This variant is missing residues 132-183 in comparison to the full-length DNAJB6b, 0.05 M sodium chloride, 0.02 % w/v sodium azide, 0.02 M sodium phosphate, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
25 | DNAJB6b deltaST variant | [U-15N;U-2H] | 0.0002 M | |
26 | sodium chloride | natural abundance | 0.05 M | |
27 | sodium azide | natural abundance | 0.02 % w/v | |
28 | sodium phosphate | natural abundance | 0.02 M |