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BMRB: 30658

6U4M

Solution structure of paxillin LIM4

Authors

Zhu, L., Qin, J.

Assembly

Paxillin

Entity

1. Paxillin, entity 1 (polymer, Thiol state: free and other bound), 72 monomers, 8295.643 Da Detail

GAMDPEFYHE RRGSLCSGCQ KPITGRCITA MAKKFHPEHF VCAFCLKQLN KGTFKEQNDK PYCQNCFLKL FC

2. Paxillin, entity ZN (non-polymer), 65.409 × 2 Da

Total weight

8426.461 Da

Max. entity weight

8295.643 Da

Entity Connection

na 8 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS16:SG	2:ZN1:ZN
2	na	sing	1:CYS19:SG	2:ZN1:ZN
3	na	sing	1:HIS36:ND1	2:ZN1:ZN
4	na	sing	1:HIS39:ND1	2:ZN1:ZN
5	na	sing	1:CYS42:SG	2:ZN1:ZN
6	na	sing	1:CYS45:SG	2:ZN1:ZN
7	na	sing	1:CYS63:SG	2:ZN1:ZN
8	na	sing	1:CYS66:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 82.2 %, Completeness (bb): 94.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.2 % (717 of 872)	81.6 % (376 of 461)	80.4 % (270 of 336)	94.7 % (71 of 75)
Backbone	94.6 % (401 of 424)	94.5 % (137 of 145)	94.8 % (200 of 211)	94.1 % (64 of 68)
Sidechain	74.2 % (382 of 515)	75.6 % (239 of 316)	70.8 % (136 of 192)	100.0 % (7 of 7)
Aromatic	5.1 % (5 of 98)	10.2 % (5 of 49)	0.0 % (0 of 49)
Methyl	91.3 % (42 of 46)	91.3 % (21 of 23)	91.3 % (21 of 23)

1. entity 1

GAMDPEFYHE RRGSLCSGCQ KPITGRCITA MAKKFHPEHF VCAFCLKQLN KGTFKEQNDK PYCQNCFLKL FC

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 null, Temperature 298 K, pH 6.8, Details 1.45 mM [U-13C; U-15N] Paxillin LIM4, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	Paxillin LIM4	[U-13C; U-15N]		1.45 mM
2	NaCl	natural abundance		50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.32 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.32 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.82 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 6U4M, Strand ID: A Detail

Release date

2019-09-12

Citation

Structural Basis of Paxillin Recruitment by Kindlin-2 in Regulating Cell Adhesion
Zhu, L., Liu, H., Lu, F., Yang, J., Byzova, T.V., Qin, J.
Structure (2019), 27, 1686-1697, PubMed 31590942 , DOI 10.1016/j.str.2019.09.006 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 30659 released on 2019-09-12
Title Solution structure of paxillin LIM4 in complex with kindlin-2 F0

Related entities 1. Paxillin, entity 1, : 1 : 1 : 4 : 279 entities Detail

Interaction partners 1. Paxillin, entity 1, : 57 interactors Detail

More details for 57 interactors identified by 30 citations

Experiments performed 7 experiments Detail

1 3D HNCO