BMRBj - BMREntryMaster

	Total	¹H
All	96.0 % (191 of 199)	96.0 % (191 of 199)
Backbone	93.4 % (57 of 61)	93.4 % (57 of 61)
Sidechain	97.1 % (134 of 138)	97.1 % (134 of 138)
Aromatic	100.0 % (24 of 24)	100.0 % (24 of 24)
Methyl	100.0 % (6 of 6)	100.0 % (6 of 6)

1. entity 1

RPFXCTWSYC GKRFTRSDEL QRHKRTHTGE KX

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.3 mM zinc finger 2 from transcription factor Sp1 DNA-binding domain, Met-Nle mutant, 10 mM [U-2H] TRIS, 0.05 mM DSS, 1.56 mM zinc chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	entity_1	natural abundance	1.3 mM
2	TRIS	[U-2H]	10 mM
3	DSS	natural abundance	0.05 mM
4	zinc chloride	natural abundance	1.56 mM

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Calculated from 10 models in PDB: 6UCP, Strand ID: A Detail

Release date

2019-09-19

Citation

Proteomimetic Zinc Finger Domains with Modified Metal-binding β-Turns
Rao, S.R., Horne, W.S.
Pept. Sci (Hoboken). (2020), 112, e24177-e24177, PubMed 33733039 , DOI 10.1002/pep2.24177 , Abstract

Related entities 1. Transcription factor Sp1, entity 1, : 1 : 11 : 668 entities Detail

Interaction partners 1. Transcription factor Sp1, entity 1, : 66 interactors Detail

More details for 66 interactors identified by 36 citations

Experiments performed 3 experiments Detail

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30674_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	True (see coodinates for details)
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

Ptnr_site_1	Ptnr_site_2	Redox_state_prediction_1	Redox_state_prediction_2	Distance (Å)
1:5:CYS:SG	2:1:ZN:ZN	unknown	unknown	n/a
1:10:CYS:SG	2:1:ZN:ZN	unknown	unknown	n/a
1:23:HIS:NE2	2:1:ZN:ZN	unknown	unknown	n/a
1:27:HIS:NE2	2:1:ZN:ZN	unknown	unknown	n/a

Non-standard residues

Chain_ID	Seq_ID	Comp_ID	Chem_comp_name	Experimental evidences
1	4	NLE	NORLEUCINE	Assigned chemical shifts
1	32	NH2	AMINO GROUP	Assigned chemical shifts
2	1	ZN	ZINC ION	None

Content subtype: bmr30674_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	211		100.0 (chain: 1, length: 32)
1	Chemical shift references	chem_shift_reference_1	OK	1		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 32, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 211
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	210	201	95.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	63	59	93.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	147	142	96.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	7	7	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	24	24	100.0

Keywords DNA binding protein, zinc finger

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Found 1 Document (1.261 seconds)

BMRB: 30674

Sample

Related entities 1. Transcription factor Sp1, entity 1, : 1 : 11 : 668 entities Detail

Interaction partners 1. Transcription factor Sp1, entity 1, : 66 interactors Detail

Experiments performed 3 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 4 contents Detail