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BMRB: 30697

Solution Structure of the Tau pre-mRNA Exon 10 Splicing Regulatory Element
Authors
Chen, J.L., Fountain, M.A., Disney, M.D.
Assembly
Tau pre-mRNA Exon 10 Splicing Regulatory Element
Entity
1. Tau pre-mRNA Exon 10 Splicing Regulatory Element, entity 1 (polymer, Thiol state: not present), 11 monomers, 3601.126 Da Detail

CCGGCAGUGU G


2. Tau pre-mRNA Exon 10 Splicing Regulatory Element, entity 2 (polymer, Thiol state: not present), 10 monomers, 3238.936 Da Detail

CACACGUCGG


Total weight
6840.062 Da
Max. entity weight
3601.126 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 100.0 %, Completeness: 50.3 %, Completeness (bb): 47.6 % Detail
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Polymer type: polyribonucleotide

Total1H
All50.3 % (93 of 185)50.3 % (93 of 185)
Suger, PO447.6 % (60 of 126)47.6 % (60 of 126)
Nucleobase55.9 % (33 of 59)55.9 % (33 of 59)
Aromatic73.3 % (33 of 45)73.3 % (33 of 45)

1. entity 1

CCGGCAGUGU G

2. entity 2

CACACGUCGG

Show sample condition
Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 0.7 mM WT Tau RNA, 10 mM KH2PO4/K2HPO4, 0.05 mM EDTA, 100% D2O


#NameIsotope labelingTypeConcentration
1WT Tau RNAnatural abundance0.7 mM
2WT Tau RNAnatural abundance0.7 mM
3KH2PO4/K2HPO4natural abundance10 mM
4EDTAnatural abundance0.05 mM
Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 0.7 mM WT Tau RNA, 10 mM KH2PO4/K2HPO4, 0.05 mM EDTA, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
5WT Tau RNAnatural abundance0.7 mM
6WT Tau RNAnatural abundance0.7 mM
7KH2PO4/K2HPO4natural abundance10 mM
8EDTAnatural abundance0.05 mM

Chem. Shift Complete2
Sequence coverage: 76.2 %, Completeness: 31.1 %, Completeness (bb): 23.8 % Detail
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Polymer type: polyribonucleotide

Total1H
All31.1 % (115 of 370)31.1 % (115 of 370)
Suger, PO423.8 % (60 of 252)23.8 % (60 of 252)
Nucleobase46.6 % (55 of 118)46.6 % (55 of 118)
Aromatic47.8 % (43 of 90)47.8 % (43 of 90)

1. entity 1

CCGGCAGUGU G

2. entity 2

CACACGUCGG

Show sample condition
Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 281.5 K, pH 6.0, Details 0.7 mM WT Tau RNA, 10 mM KH2PO4/K2HPO4, 0.05 mM EDTA, 100% D2O


#NameIsotope labelingTypeConcentration
1WT Tau RNAnatural abundance0.7 mM
2WT Tau RNAnatural abundance0.7 mM
3KH2PO4/K2HPO4natural abundance10 mM
4EDTAnatural abundance0.05 mM
Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 281.5 K, pH 6.0, Details 0.7 mM WT Tau RNA, 10 mM KH2PO4/K2HPO4, 0.05 mM EDTA, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
5WT Tau RNAnatural abundance0.7 mM
6WT Tau RNAnatural abundance0.7 mM
7KH2PO4/K2HPO4natural abundance10 mM
8EDTAnatural abundance0.05 mM

Release date
2020-01-16
Citation
Design, Optimization, and Study of Small Molecules That Target Tau Pre-mRNA and Affect Splicing
Chen, J.L., Zhang, P., Abe, M., Aikawa, H., Zhang, L., Frank, A.J., Zembryski, T., Hubbs, C., Park, H., Withka, J., Steppan, C., Rogers, L., Cabral, S., Pettersson, M., Wager, T.T., Fountain, M.A., Rumbaugh, G., Childs-Disney, J.L., Disney, M.D.
J. Am. Chem. Soc. (2020), 142, 8706-8727, PubMed 32364710 , DOI 10.1021/jacs.0c00768 ,
Entries sharing articles BMRB: 3 entries Detail
  BMRB: 30698 released on 2020-01-16
    Title Solution Structure of the Tau pre-mRNA Exon 10 Splicing Regulatory Element Bound to MH5
  BMRB: 30699 released on 2020-01-16
    Title Solution Structure of the Tau pre-mRNA Exon 10 Splicing Regulatory Element Bound to MQC
  BMRB: 30700 released on 2020-01-06
    Title Solution Structure of the Tau pre-mRNA Exon 10 Splicing Regulatory Element Bound to MIP
Experiments performed 3 experiments Detail
Chemical shift validation 5 contents Detail
Keywords A-form tau RNA hairpin, Adenine bulge, RNA, RNA complex