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BMRB: 30699

Solution Structure of the Tau pre-mRNA Exon 10 Splicing Regulatory Element Bound to MQC
Authors
Chen, J.L., Fountain, M.A., Disney, M.D.
Assembly
Tau pre-mRNA Exon 10 Splicing Regulatory Element Bound to MQC
Entity
1. Tau pre-mRNA Exon 10 Splicing Regulatory Element Bound to MQC, entity 1 (polymer, Thiol state: not present), 11 monomers, 3601.126 Da Detail

CCGGCAGUGU G


2. Tau pre-mRNA Exon 10 Splicing Regulatory Element Bound to MQC, entity 2 (polymer, Thiol state: not present), 10 monomers, 3238.936 Da Detail

CACACGUCGG


3. Tau pre-mRNA Exon 10 Splicing Regulatory Element Bound to MQC, entity MQC (non-polymer), 306.362 Da
Total weight
7146.424 Da
Max. entity weight
3601.126 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 100.0 %, Completeness: 63.2 %, Completeness (bb): 66.7 % Detail
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Polymer type: polyribonucleotide

Total1H
All63.2 % (117 of 185)63.2 % (117 of 185)
Suger, PO466.7 % (84 of 126)66.7 % (84 of 126)
Nucleobase55.9 % (33 of 59)55.9 % (33 of 59)
Aromatic73.3 % (33 of 45)73.3 % (33 of 45)

1. entity 1

CCGGCAGUGU G

2. entity 2

CACACGUCGG

Show sample condition
Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0, Details 0.4 mM WT Tau RNA, 0.6 mM MQC, 10 mM KH2PO4/K2HPO4, 0.05 mM EDTA, 100% D2O


#NameIsotope labelingTypeConcentration
1WT Tau RNAnatural abundance0.4 mM
2WT Tau RNAnatural abundance0.4 mM
3MQCnatural abundance0.6 mM
4KH2PO4/K2HPO4natural abundance10 mM
5EDTAnatural abundance0.05 mM
Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0, Details 0.4 mM WT Tau RNA, 0.6 mM MQC, 10 mM KH2PO4/K2HPO4, 0.05 mM EDTA, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
6WT Tau RNAnatural abundance0.4 mM
7WT Tau RNAnatural abundance0.4 mM
8MQCnatural abundance0.6 mM
9KH2PO4/K2HPO4natural abundance10 mM
10EDTAnatural abundance0.05 mM

Chem. Shift Complete2
Sequence coverage: 76.2 %, Completeness: 37.6 %, Completeness (bb): 33.3 % Detail
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Polymer type: polyribonucleotide

Total1H
All37.6 % (139 of 370)37.6 % (139 of 370)
Suger, PO433.3 % (84 of 252)33.3 % (84 of 252)
Nucleobase46.6 % (55 of 118)46.6 % (55 of 118)
Aromatic47.8 % (43 of 90)47.8 % (43 of 90)

1. entity 1

CCGGCAGUGU G

2. entity 2

CACACGUCGG

Show sample condition
Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0, Details 0.4 mM WT Tau RNA, 0.6 mM MQC, 10 mM KH2PO4/K2HPO4, 0.05 mM EDTA, 100% D2O


#NameIsotope labelingTypeConcentration
1WT Tau RNAnatural abundance0.4 mM
2WT Tau RNAnatural abundance0.4 mM
3MQCnatural abundance0.6 mM
4KH2PO4/K2HPO4natural abundance10 mM
5EDTAnatural abundance0.05 mM
Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0, Details 0.4 mM WT Tau RNA, 0.6 mM MQC, 10 mM KH2PO4/K2HPO4, 0.05 mM EDTA, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
6WT Tau RNAnatural abundance0.4 mM
7WT Tau RNAnatural abundance0.4 mM
8MQCnatural abundance0.6 mM
9KH2PO4/K2HPO4natural abundance10 mM
10EDTAnatural abundance0.05 mM

Release date
2020-01-16
Citation
Design, Optimization, and Study of Small Molecules That Target Tau Pre-mRNA and Affect Splicing
Chen, J.L., Zhang, P., Abe, M., Aikawa, H., Zhang, L., Frank, A.J., Zembryski, T., Hubbs, C., Park, H., Withka, J., Steppan, C., Rogers, L., Cabral, S., Pettersson, M., Wager, T.T., Fountain, M.A., Rumbaugh, G., Childs-Disney, J.L., Disney, M.D.
J. Am. Chem. Soc. (2020), 142, 8706-8727, PubMed 32364710 , DOI 10.1021/jacs.0c00768 ,
Experiments performed 3 experiments Detail
Chemical shift validation 6 contents Detail
Keywords A-form tau RNA hairpin, Adenine bulge, RNA, RNA complex