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BMRB: 30700

Solution Structure of the Tau pre-mRNA Exon 10 Splicing Regulatory Element Bound to MIP
Authors
Chen, J.L., Fountain, M.A., Disney, M.D.
Assembly
Tau pre-mRNA Exon 10 Splicing Regulatory Element Bound to MIP
Entity
1. Tau pre-mRNA Exon 10 Splicing Regulatory Element Bound to MIP, entity 1 (polymer, Thiol state: not present), 11 monomers, 3601.126 Da Detail

CCGGCAGUGU G


2. Tau pre-mRNA Exon 10 Splicing Regulatory Element Bound to MIP, entity 2 (polymer, Thiol state: not present), 10 monomers, 3238.936 Da Detail

CACACGUCGG


3. Tau pre-mRNA Exon 10 Splicing Regulatory Element Bound to MIP, entity QSY (non-polymer), 369.481 Da
Total weight
7209.543 Da
Max. entity weight
3601.126 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 100.0 %, Completeness: 50.8 %, Completeness (bb): 48.4 % Detail
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Polymer type: polyribonucleotide

Total1H
All50.8 % (94 of 185)50.8 % (94 of 185)
Suger, PO448.4 % (61 of 126)48.4 % (61 of 126)
Nucleobase55.9 % (33 of 59)55.9 % (33 of 59)
Aromatic73.3 % (33 of 45)73.3 % (33 of 45)

1. entity 1

CCGGCAGUGU G

2. entity 2

CACACGUCGG

Show sample condition
Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 0.4 mM WT Tau RNA, 0.6 mM MIP, 10 mM KH2PO4/K2HPO4, 0.05 mM EDTA, 100% D2O


#NameIsotope labelingTypeConcentration
1entity_1natural abundance0.4 mM
2entity_2natural abundance0.4 mM
3MIPnatural abundance0.6 mM
4KH2PO4/K2HPO4natural abundance10 mM
5EDTAnatural abundance0.05 mM
Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 0.4 mM WT Tau RNA, 0.6 mM MIP, 10 mM KH2PO4/K2HPO4, 0.05 mM EDTA, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
6entity_1natural abundance0.4 mM
7entity_2natural abundance0.4 mM
8MIPnatural abundance0.6 mM
9KH2PO4/K2HPO4natural abundance10 mM
10EDTAnatural abundance0.05 mM

Chem. Shift Complete2
Sequence coverage: 76.2 %, Completeness: 31.4 %, Completeness (bb): 24.2 % Detail
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Polymer type: polyribonucleotide

Total1H
All31.4 % (116 of 370)31.4 % (116 of 370)
Suger, PO424.2 % (61 of 252)24.2 % (61 of 252)
Nucleobase46.6 % (55 of 118)46.6 % (55 of 118)
Aromatic47.8 % (43 of 90)47.8 % (43 of 90)

1. entity 1

CCGGCAGUGU G

2. entity 2

CACACGUCGG

Show sample condition
Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 0.4 mM WT Tau RNA, 0.6 mM MIP, 10 mM KH2PO4/K2HPO4, 0.05 mM EDTA, 100% D2O


#NameIsotope labelingTypeConcentration
1entity_1natural abundance0.4 mM
2entity_2natural abundance0.4 mM
3MIPnatural abundance0.6 mM
4KH2PO4/K2HPO4natural abundance10 mM
5EDTAnatural abundance0.05 mM
Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 0.4 mM WT Tau RNA, 0.6 mM MIP, 10 mM KH2PO4/K2HPO4, 0.05 mM EDTA, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
6entity_1natural abundance0.4 mM
7entity_2natural abundance0.4 mM
8MIPnatural abundance0.6 mM
9KH2PO4/K2HPO4natural abundance10 mM
10EDTAnatural abundance0.05 mM

Release date
2020-01-06
Citation
Design, Optimization, and Study of Small Molecules That Target Tau Pre-mRNA and Affect Splicing
Chen, J.L., Zhang, P., Abe, M., Aikawa, H., Zhang, L., Frank, A.J., Zembryski, T., Hubbs, C., Park, H., Withka, J., Steppan, C., Rogers, L., Cabral, S., Pettersson, M., Wager, T.T., Fountain, M.A., Rumbaugh, G., Childs-Disney, J.L., Disney, M.D.
J. Am. Chem. Soc. (2020), 142, 8706-8727, PubMed 32364710 , DOI 10.1021/jacs.0c00768 ,
Entries sharing articles BMRB: 3 entries Detail
  BMRB: 30697 released on 2020-01-16
    Title Solution Structure of the Tau pre-mRNA Exon 10 Splicing Regulatory Element
  BMRB: 30698 released on 2020-01-16
    Title Solution Structure of the Tau pre-mRNA Exon 10 Splicing Regulatory Element Bound to MH5
  BMRB: 30699 released on 2020-01-16
    Title Solution Structure of the Tau pre-mRNA Exon 10 Splicing Regulatory Element Bound to MQC
Experiments performed 3 experiments Detail
Chemical shift validation 6 contents Detail
Keywords A-form tau RNA hairpin, Adenine bulge, MIP, RNA, RNA complex