Solution structure of the PHD of mouse UHRF1 (NP95)
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:CYS5:SG | 2:ZN1:ZN |
2 | na | sing | 1:CYS8:SG | 2:ZN1:ZN |
3 | na | sing | 1:CYS16:SG | 2:ZN1:ZN |
4 | na | sing | 1:CYS19:SG | 2:ZN1:ZN |
5 | na | sing | 1:CYS21:SG | 2:ZN1:ZN |
6 | na | sing | 1:CYS24:SG | 2:ZN1:ZN |
7 | na | sing | 1:HIS44:ND1 | 2:ZN1:ZN |
8 | na | sing | 1:CYS47:SG | 2:ZN1:ZN |
9 | na | sing | 1:CYS36:SG | 2:ZN1:ZN |
10 | na | sing | 1:GLU38:OE2 | 2:ZN1:ZN |
11 | na | sing | 1:CYS39:SG | 2:ZN1:ZN |
12 | na | sing | 1:CYS63:SG | 2:ZN1:ZN |
13 | na | sing | 1:CYS66:SG | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 94.8 % (820 of 865) | 96.5 % (441 of 457) | 91.9 % (308 of 335) | 97.3 % (71 of 73) |
Backbone | 96.0 % (432 of 450) | 98.7 % (149 of 151) | 93.5 % (215 of 230) | 98.6 % (68 of 69) |
Sidechain | 91.8 % (449 of 489) | 93.1 % (285 of 306) | 89.9 % (161 of 179) | 75.0 % (3 of 4) |
Aromatic | 67.9 % (38 of 56) | 82.1 % (23 of 28) | 51.9 % (14 of 27) | 100.0 % (1 of 1) |
Methyl | 91.7 % (44 of 48) | 91.7 % (22 of 24) | 91.7 % (22 of 24) |
1. entity 1
SGPSCRFCKD DENKPCRKCA CHVCGGREAP EKQLLCDECD MAFHLYCLKP PLTSVPPEPE WYCPSCRTDS SEVVQAGESolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 290 K, pH 7.5, Details 200 uM [U-99% 13C; U-99% 15N] PHD, 5 mM DTT, 2 mM beta-mercaptoethanol, 5 mM TCEP, 150 mM sodium chloride, 50 mM sodium phosphate, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | PHD | [U-99% 13C; U-99% 15N] | 200 uM | |
2 | DTT | natural abundance | 5 mM | |
3 | beta-mercaptoethanol | natural abundance | 2 mM | |
4 | TCEP | natural abundance | 5 mM | |
5 | sodium chloride | natural abundance | 150 mM | |
6 | sodium phosphate | natural abundance | 50 mM |