BMRB: 30723

Cap3G-TAR-F1

Authors

Summers, M.F., Brown, J.D.

Assembly

RNA (34-MER)

Entity

1. RNA (34-MER) (polymer, Thiol state: not present), 34 monomers, 11104.53 Da Detail

XGGGUCUCUC UUGUCGAGAG GCAAGGAACC CACU

Formula weight

11104.53 Da

Source organism

Human immunodeficiency virus 1

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.1 %, Completeness: 54.0 %, Completeness (bb): 50.5 % Detail

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Polymer type: polyribonucleotide

	Total	1H
All	54.0 % (156 of 289)	54.0 % (156 of 289)
Suger, PO4	50.5 % (100 of 198)	50.5 % (100 of 198)
Nucleobase	61.5 % (56 of 91)	61.5 % (56 of 91)
Aromatic	76.7 % (56 of 73)	76.7 % (56 of 73)

1. entity 1

XGGGUCUCUC UUGUCGAGAG GCAAGGAACC CACU

Show sample condition

Sample

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.4, Details 150 uM Fully protonated Cap3G-TAR-F1, 10 mM Deuterated KH2PO4, 100% D2O

#	Name	Isotope labeling	Type	Concentration
1	Cap3G-TAR-F1	Fully protonated		150 uM
2	KH2PO4	Deuterated		10 mM

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Release date

2020-04-06

Citation

Structural basis for transcriptional start site control of HIV-1 RNA fate
Brown, J.D., Kharytonchyk, S., Chaudry, I., Iyer, A.S., Carter, H., Becker, G., Desai, Y., Glang, L., Choi, S.H., Singh, K., Lopresti, M.W., Orellana, M., Rodriguez, T., Oboh, U., Hijji, J., Ghinger, F.G., Stewart, K., Francis, D., Edwards, B., Chen, P., Case, D.A., Telesnitsky, A., Summers, M.F.
Science (2020), 368, 413-417, PubMed 32327595 , DOI 10.1126/science.aaz7959 , Abstract

Related entities 1. RNA (34-MER), : 1 entities Detail

Experiments performed 2 experiments Detail