BMRB: 30725

Solution structure of the N-terminal helix-hairpin-helix domain of human MUS81

Authors

Payliss, B., Houliston, S., Lemak, A., Arrowsmith, C.H., Wyatt, H.D.M.

Assembly

Crossover junction endonuclease MUS81 (E.C.3.1.22.-)

Entity

1. Crossover junction endonuclease MUS81 (E.C.3.1.22.-) (polymer, Thiol state: all free), 90 monomers, 10594.21 Da Detail

AAAPVRLGRK RPLPACPNPL FVRWLTEWRD EATRSRRRTR FVFQKALRSL RRYPLPLRSG KEAKILQHFG DGLCRMLDER LQRHRTSGGD

Formula weight

10594.21 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 89.5 %, Completeness (bb): 92.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.5 % (1001 of 1118)	90.8 % (535 of 589)	90.8 % (383 of 422)	77.6 % (83 of 107)
Backbone	92.6 % (487 of 526)	93.3 % (167 of 179)	92.0 % (243 of 264)	92.8 % (77 of 83)
Sidechain	88.2 % (596 of 676)	89.8 % (368 of 410)	91.7 % (222 of 242)	25.0 % (6 of 24)
Aromatic	80.0 % (64 of 80)	92.5 % (37 of 40)	65.8 % (25 of 38)	100.0 % (2 of 2)
Methyl	95.3 % (82 of 86)	95.3 % (41 of 43)	95.3 % (41 of 43)

1. entity 1

AAAPVRLGRK RPLPACPNPL FVRWLTEWRD EATRSRRRTR FVFQKALRSL RRYPLPLRSG KEAKILQHFG DGLCRMLDER LQRHRTSGGD

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 350 uM [U-13C; U-15N] Labeled Protein, 25 mM sodium phosphate, 100 mM sodium chloride, 100 uM EDTA, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	Labeled Protein	[U-13C; U-15N]		350 uM
2	sodium phosphate	natural abundance		25 mM
3	sodium chloride	natural abundance		100 mM
4	EDTA	natural abundance		100 uM

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Protein Blocks Logo

Calculated from 20 models in PDB: 6VWB, Strand ID: A Detail

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Release date

2020-05-05

Citation

Phosphorylation of the DNA repair scaffold SLX4 drives folding of the SAP domain and activation of the MUS81-EME1 endonuclease
Payliss, B.J., Tse, Y., Reichheld, S.E., Lemak, A., Yun, H.Y., Houliston, S., Patel, A., Arrowsmith, C.H., Sharpe, S., Wyatt, H.
Cell Rep. (2022), 41, 111537-111537, PubMed 36288699 , DOI 10.1016/j.celrep.2022.111537 , Abstract

Related entities 1. Crossover junction endonuclease MUS81 (E.C.3.1.22.-), : 1 : 3 : 13 entities Detail

Interaction partners 1. Crossover junction endonuclease MUS81 (E.C.3.1.22.-), : 16 interactors Detail

Experiments performed 10 experiments Detail