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BMRB: 30735


30735
2MSE
NMR-driven structure of KRAS4B-GDP homodimer on a lipid bilayer nanodisc
Authors
Lee, K., Fang, Z., Enomoto, M., Gasmi-Seabrook, G.M., Zheng, L., Marshall, C.B., Ikura, M.
Assembly
Apolipoprotein A-I, GTPase KRas
Entity
1. Apolipoprotein A-I, GTPase KRas, entity 1 (polymer), 200 monomers, 23196.96 × 2 Da Detail

GPLKLLDNWD SVTSTFSKLR EQLGPVTQEF WDNLEKETEG LRQEMSKDLE EVKAKVQPYL DDFQKKWQEE MELYRQKVEP LRAELQEGAR QKLHELQEKL SPLGEEMRDR ARAHVDALRT HLAPYSDELR QRLAARLEAL KENGGARLAE YHAKATEHLS TLSEKAKPAL EDLRQGLLPV LESFKVSFLS ALEEYTKKLN


2. Apolipoprotein A-I, GTPase KRas, entity 2 (polymer), 184 monomers, 21007.64 × 2 Da Detail

TEYKLVVVGA DGVGKSALTI QLIQNHFVDE YDPTIEDSYR KQVVIDGETC LLDILDTAGQ EEYSAMRDQY MRTGEGFLCV FAINNTKSFE DIHHYREQIK RVKDSEDVPM VLVGNKCDLP SRTVDTKQAQ DLARSYGIPF IETSAKTRQG VDDAFYTLVR EIRKHKEKMS KDGKKKKKKS KTKC


3. Apolipoprotein A-I, GTPase KRas, entity PCW (non-polymer), 787.121 × 64 Da
4. Apolipoprotein A-I, GTPase KRas, entity 17F (non-polymer), 788.043 × 16 Da
5. Apolipoprotein A-I, GTPase KRas, entity GDP (non-polymer), 443.201 × 2 Da
6. Apolipoprotein A-I, GTPase KRas, entity MG (non-polymer), 24.305 × 2 Da
Total weight
152328.64 Da
Max. entity weight
23196.96 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 16.1 %, Completeness: 5.8 %, Completeness (bb): 4.9 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All 5.8 % (265 of 4589) 5.9 % (142 of 2415) 5.3 % (94 of 1770) 7.2 % (29 of 404)
Backbone 4.9 % (111 of 2280) 5.9 % (46 of 775) 3.3 % (37 of 1133) 7.5 % (28 of 372)
Sidechain 6.3 % (169 of 2674) 6.0 % (99 of 1640) 6.9 % (69 of 1002) 3.1 % (1 of 32)
Aromatic 0.0 % (0 of 286) 0.0 % (0 of 143) 0.0 % (0 of 140) 0.0 % (0 of 3)
Methyl22.2 % (90 of 406)22.7 % (46 of 203)21.7 % (44 of 203)

1. entity 1

GPLKLLDNWD SVTSTFSKLR EQLGPVTQEF WDNLEKETEG LRQEMSKDLE EVKAKVQPYL DDFQKKWQEE MELYRQKVEP LRAELQEGAR QKLHELQEKL SPLGEEMRDR ARAHVDALRT HLAPYSDELR QRLAARLEAL KENGGARLAE YHAKATEHLS TLSEKAKPAL EDLRQGLLPV LESFKVSFLS ALEEYTKKLN

2. entity 2

TEYKLVVVGA DGVGKSALTI QLIQNHFVDE YDPTIEDSYR KQVVIDGETC LLDILDTAGQ EEYSAMRDQY MRTGEGFLCV FAINNTKSFE DIHHYREQIK RVKDSEDVPM VLVGNKCDLP SRTVDTKQAQ DLARSYGIPF IETSAKTRQG VDDAFYTLVR EIRKHKEKMS KDGKKKKKKS KTKC

Show sample condition
Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 7.4, Details 80 uM ILV 13C-methyl; Lys 15N-amide KRAS4B, 90% H2O/10% D2O


#NameIsotope labelingTypeConcentration
1KRAS4B[ILV 13C-methyl; Lys 15N-amide]80 uM

Protein Blocks Logo
Calculated from 20 models in PDB: 6W4F, Strand ID: A, B, C, D Detail

Release date
2020-04-20
Citation
Two Distinct Structures of Membrane-Associated Homodimers of GTP- and GDP-Bound KRAS4B Revealed by Paramagnetic Relaxation Enhancement
Lee, K.Y., Fang, Z., Enomoto, M., Seabrook, G.G., Zheng, L., Koide, S., Ikura, M., Marshall, C.B.
Angew. Chem Int Ed Engl. (2020), 59, 11037-11045, PubMed 32227412 , DOI 10.1002/anie.202001758 ,
Related entities 1. Apolipoprotein A-I, GTPase KRas, entity 1, : 1 : 9 : 11 : 109 entities Detail
More details for 130 related entities
Related entities 2. Apolipoprotein A-I, GTPase KRas, entity 2, : 100 entities Detail
More details for 100 related entities
Interaction partners 1. Apolipoprotein A-I, GTPase KRas, entity 1, : 37 interactors Detail
More details for 37 interactors identified by 22 citations
Experiments performed 2 experiments Detail
Chemical shift validation 8 contents Detail
Keywords GTPase, ONCOPROTEIN, nanodisc