BMRB: 30741

Atomic-Resolution Structure of HIV-1 Capsid Tubes by Magic Angle Spinning NMR

Authors

Lu, M., Russell, R.W., Bryer, A., Quinn, C.M., Hou, G., Zhang, H., Schwieters, C.D., Perilla, J.R., Gronenborn, A.M., Polenova, T.

Assembly

HIV-1 capsid protein

Entity

1. HIV-1 capsid protein (polymer), 231 monomers, 25602.16 Da Detail

PIVQNLQGQM VHQAISPRTL NAWVKVVEEK AFSPEVIPMF SALSEGATPQ DLNTMLNTVG GHQAAMQMLK ETINEEAAEW DRLHPVHAGP IAPGQMREPR GSDIAGTTST LQEQIGWMTH NPPIPVGEIY KRWIILGLNK IVRMYSPTSI LDIRQGPKEP FRDYVDRFYK TLRAEQASQE VKNWMTETLL VQNANPDCKT ILKALGPGAT LEEMMTACQG VGGPGHKARV L

Formula weight

25602.16 Da

Source organism

Human immunodeficiency virus 1

Exptl. method

solid-state NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.0 %, Completeness: 83.4 %, Completeness (bb): 92.1 % Detail

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Polymer type: polypeptide(L)

	Total	13C	15N
All	83.4 % (1077 of 1292)	84.4 % (876 of 1038)	79.1 % (201 of 254)
Backbone	92.1 % (818 of 888)	91.4 % (617 of 675)	94.4 % (201 of 213)
Sidechain	75.0 % (463 of 617)	80.4 % (463 of 576)	0.0 % (0 of 41)
Aromatic	51.3 % (40 of 78)	54.8 % (40 of 73)	0.0 % (0 of 5)
Methyl	85.5 % (112 of 131)	85.5 % (112 of 131)

1. entity 1

PIVQNLQGQM VHQAISPRTL NAWVKVVEEK AFSPEVIPMF SALSEGATPQ DLNTMLNTVG GHQAAMQMLK ETINEEAAEW DRLHPVHAGP IAPGQMREPR GSDIAGTTST LQEQIGWMTH NPPIPVGEIY KRWIILGLNK IVRMYSPTSI LDIRQGPKEP FRDYVDRFYK TLRAEQASQE VKNWMTETLL VQNANPDCKT ILKALGPGAT LEEMMTACQG VGGPGHKARV L

Show sample condition

Sample #1

Solvent system solid, Pressure 1 atm, Temperature 277 K, pH 6, Details 100 % [U-13C; U-15N] HIV-1 capsid protein, solid

#	Name	Isotope labeling	Type	Concentration
1	HIV-1 capsid protein	[U-13C; U-15N]		100 %

Sample #2

Solvent system solid, Pressure 1 atm, Temperature 277 K, pH 6, Details 100 % [1,6-13C]-Glucose,U-15N HIV-1 capsid protein, solid

#	Name	Isotope labeling	Type	Concentration
2	HIV-1 capsid protein	[1,6-13C]-Glucose; U-15N		100 %

Sample #3

Solvent system solid, Pressure 1 atm, Temperature 277 K, pH 6, Details 100 % [2-13C]-Glucose,U-15N HIV-1 capsid protein, solid

#	Name	Isotope labeling	Type	Concentration
3	HIV-1 capsid protein	[2-13C]-Glucose; U-15N		100 %

Sample #4

Solvent system solid, Pressure 1 atm, Temperature 277 K, pH 6, Details 100 % 13C,15N-His HIV-1 capsid protein, solid

#	Name	Isotope labeling	Type	Concentration
4	HIV-1 capsid protein	[13C,15N-His]		100 %

Sample #5

Solvent system solid, Pressure 1 atm, Temperature 277 K, pH 6, Details 100 % 13C,15N-Tyr HIV-1 capsid protein, solid

#	Name	Isotope labeling	Type	Concentration
5	HIV-1 capsid protein	[13C,15N-Tyr]		100 %

Sample #6

Solvent system solid, Pressure 1 atm, Temperature 277 K, pH 6, Details 50 % 13C,15N-Ala HIV-1 capsid protein, 50 % 13C,15N-Ile HIV-1 capsid protein, solid

#	Name	Isotope labeling	Type	Concentration
6	13C,15N-Ala HIV-1 capsid protein	[13C,15N-Ala]		50 %
7	13C,15N-Ile HIV-1 capsid protein	[13C,15N-Ile]		50 %

Sample #7

Solvent system solid, Pressure 1 atm, Temperature 277 K, pH 6, Details 50 % 13C,15N-Ala HIV-1 capsid protein, 50 % 13C,15N-Val HIV-1 capsid protein, solid

#	Name	Isotope labeling	Type	Concentration
8	13C,15N-Ala HIV-1 capsid protein	[13C,15N-Ala]		50 %
9	13C,15N-Val HIV-1 capsid protein	[13C,15N-Val]		50 %

Sample #8

Solvent system solid, Pressure 1 atm, Temperature 277 K, pH 6, Details 86 % HIV-1 capsid protein, 14 % U-13C,15N-CA HIV-1 capsid protein, solid

#	Name	Isotope labeling	Type	Concentration
10	HIV-1 capsid protein	natural abundance		86 %
11	U-13C,15N-CA HIV-1 capsid protein	[U-13C; 15N-CA]		14 %

Sample #9

Solvent system solid, Pressure 1 atm, Temperature 277 K, pH 6, Details 50 % [U-13C] HIV-1 capsid protein, 50 % [U-15N] HIV-1 capsid protein, solid

#	Name	Isotope labeling	Type	Concentration
12	[U-13C] HIV-1 capsid protein	[U-13C]		50 %
13	[U-15N] HIV-1 capsid protein	[U-15N]		50 %

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Release date

2020-08-06

Citation

Atomic-resolution structure of HIV-1 capsid tubes by magic-angle spinning NMR
Lu, M., Russell, R.W., Bryer, A., Quinn, C.M., Hou, G., Zhang, H., Schwieters, C.D., Perilla, J.R., Gronenborn, A.M., Polenova, T.
Nat. Struct. Mol. Biol. (2020), 27, 863-869, PubMed 32901160 , DOI 10.1038/s41594-020-0489-2 , Abstract

Related entities 1. HIV-1 capsid protein, : 1 : 30 : 2 : 66 : 124 entities Detail

Interaction partners 1. HIV-1 capsid protein, : 4 interactors Detail

Experiments performed 37 experiments Detail