BMRB: 30746

NMR Structure of HSP-4 antimicrobial peptide in presence of DPC-d38 micelles

Authors

Verly, R.M., Nunes, L.O.

Assembly

Hylaseptin-4

Entity

1. Hylaseptin-4 (polymer, Thiol state: not present), 26 monomers, 2415.809 Da Detail

GIGDILKNLA KAAGKAALHA VGESLX

Formula weight

2415.809 Da

Source organism

Boana punctata

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 96.2 %, Completeness: 48.1 %, Completeness (bb): 65.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	48.1 % (126 of 262)	42.9 % (57 of 133)	42.7 % (44 of 103)	96.2 % (25 of 26)
Backbone	65.3 % (98 of 150)	53.7 % (29 of 54)	62.0 % (44 of 71)	100.0 % (25 of 25)
Sidechain	32.3 % (43 of 133)	35.4 % (28 of 79)	28.3 % (15 of 53)	0.0 % (0 of 1)
Aromatic	0.0 % (0 of 4)	0.0 % (0 of 2)	0.0 % (0 of 2)
Methyl	27.5 % (11 of 40)	50.0 % (10 of 20)	5.0 % (1 of 20)

1. entity 1

GIGDILKNLA KAAGKAALHA VGESLX

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Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 5 mM HSP-4, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	HSP-4	natural abundance		5 mM

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Release date

2020-05-31

Citation

High-resolution structural profile of hylaseptin-4: Aggregation, membrane topology and pH dependence of overall membrane binding process
Nunes, L.O., Munhoz, V., Sousa, A.A., de Souza, K.R., Santos, T.L., Bemquerer, M.P., Ferreira, D., de Magalhaes, M., Resende, J.M., Alcantara, A., Aisenbrey, C., Veloso, D.P., Bechinger, B., Verly, R.M.
Biochim. Biophys. Acta. Biomembr. (2021), 1863, 183581-183581, PubMed 33556358 , DOI 10.1016/j.bbamem.2021.183581 , Abstract

Related entities 1. Hylaseptin-4, : 1 : 16 entities Detail

Experiments performed 4 experiments Detail