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BMRB: 30754

6X46

NMR solution structure of Asterix/Gtsf1 from mouse (CHHC zinc finger domains)

Authors

Ipsaro, J.J., O'Brien, P.A., Bhattacharya, S., Palmer III, A.G., Joshua-Tor, L.

Assembly

Gametocyte-specific factor 1

Entity

1. Gametocyte-specific factor 1, entity 1 (polymer, Thiol state: all other bound), 121 monomers, 14198.45 Da Detail

MEDTYIDSLD PEKLLQCPYD KNHQIRASRF PYHLIKCRKN HPDVANKLAT CPFNARHQVP RAEISHHISS CDDKSSIEQD VVNQTRNLGQ ETLAESTWQS PPSDEDWDKD LWEQTENLYF Q

2. Gametocyte-specific factor 1, entity ZN (non-polymer), 65.409 × 2 Da

Total weight

14329.269 Da

Max. entity weight

14198.45 Da

Entity Connection

na 8 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS17:SG	2:ZN1:ZN
2	na	sing	1:HIS23:SG	2:ZN1:ZN
3	na	sing	1:HIS33:SG	2:ZN1:ZN
4	na	sing	1:CYS37:SG	2:ZN1:ZN
5	na	sing	1:CYS51:SG	2:ZN1:ZN
6	na	sing	1:HIS57:SG	2:ZN1:ZN
7	na	sing	1:HIS67:SG	2:ZN1:ZN
8	na	sing	1:CYS71:SG	2:ZN1:ZN

Source organism

Mus musculus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 68.6 %, Completeness: 59.0 %, Completeness (bb): 65.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	59.0 % (849 of 1440)	57.0 % (430 of 754)	61.3 % (336 of 548)	60.1 % (83 of 138)
Backbone	65.4 % (464 of 710)	66.0 % (155 of 235)	64.6 % (234 of 362)	66.4 % (75 of 113)
Sidechain	54.6 % (464 of 850)	53.0 % (275 of 519)	59.2 % (181 of 306)	32.0 % (8 of 25)
Aromatic	30.3 % (37 of 122)	31.1 % (19 of 61)	31.0 % (18 of 58)	0.0 % (0 of 3)
Methyl	66.0 % (66 of 100)	66.0 % (33 of 50)	66.0 % (33 of 50)

1. entity 1

MEDTYIDSLD PEKLLQCPYD KNHQIRASRF PYHLIKCRKN HPDVANKLAT CPFNARHQVP RAEISHHISS CDDKSSIEQD VVNQTRNLGQ ETLAESTWQS PPSDEDWDKD LWEQTENLYF Q

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.5 mM [U-15N] Gtsf1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Gtsf1	[U-15N]		0.5 mM
2	NaCl	natural abundance		200 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.8 mM [U-13C; U-15N] Gtsf1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	Gtsf1	[U-13C; U-15N]		0.8 mM
4	NaCl	natural abundance		200 mM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.8 mM [U-13C; U-15N] Gtsf1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
5	Gtsf1	[U-13C; U-15N]		0.8 mM
6	NaCl	natural abundance		200 mM

Release date

2020-06-28

Citation

Asterix/Gtsf1 links tRNAs and piRNA silencing of retrotransposons
Ipsaro, J.J., O'Brien, P.A., Bhattacharya, S., Palmer III, A.G., Joshua-Tor, L.
Cell Rep. (2021), 34, 108914-108914, PubMed 33789107 , DOI 10.1016/j.celrep.2021.108914 , Abstract

Entries sharing articles EMDB: 2 entries Detail

  EMDB: 22040 released on 2021-03-03
    Title Asterix/Gtsf1 from mouse (full-length protein) bound to co-purifying tRNA
  EMDB: 22041 released on 2021-03-03
    Title Asterix/Gtsf1 from mouse (residues 1-45; zinc finger 1) bound to co-purifying tRNA

Related entities 1. Gametocyte-specific factor 1, entity 1, : 1 : 19 entities Detail

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.5 mM [U-15N] Gtsf1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Gtsf1	[U-15N]		0.5 mM
2	NaCl	natural abundance		200 mM

2 2D 1H-13C HSQC

Instrument

Bruker AVANCE - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.8 mM [U-13C; U-15N] Gtsf1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
5	Gtsf1	[U-13C; U-15N]		0.8 mM
6	NaCl	natural abundance		200 mM

3 3D HNCA