BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	67.2 % (354 of 527)	75.4 % (202 of 268)	53.1 % (112 of 211)	83.3 % (40 of 48)
Backbone	74.6 % (200 of 268)	86.7 % (78 of 90)	61.9 % (83 of 134)	88.6 % (39 of 44)
Sidechain	63.7 % (193 of 303)	69.7 % (124 of 178)	56.2 % (68 of 121)	25.0 % (1 of 4)
Aromatic	0.0 % (0 of 46)	0.0 % (0 of 23)	0.0 % (0 of 23)
Methyl	79.3 % (46 of 58)	89.7 % (26 of 29)	69.0 % (20 of 29)

1. entity 1

MISDEQLNSL AITFGIVMMT LIVIYHAVDS TMSPKNRLEH HHHHH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 400 uM [U-13C; U-15N] Yeast Oligosaccharyltransferase subunit Ost4, 100 mM DPC micelle, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Yeast Oligosaccharyltransferase subunit Ost4	[U-13C; U-15N]		400 uM
2	DPC micelle	natural abundance		100 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 6XCR, Strand ID: A Detail

Release date

2020-06-21

Citation

NMR and MD Simulations Reveal the Impact of the V23D Mutation on the Function of Yeast Oligosaccharyltransferase Subunit Ost4
Chaudhary, B.P., Zoetewey, D.L., McCullagh, M.J., Mohanty, S.
Glycobiology (2021), 31, 838-850, PubMed 33442744 , DOI 10.1093/glycob/cwab002 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 30761 released on 2020-06-21
Title NMR structure of Ost4 in DPC micelles

Related entities 1. Oligosaccharyltransferase, : 1 : 4 : 17 entities Detail

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 400 uM [U-13C; U-15N] Yeast Oligosaccharyltransferase subunit Ost4, 100 mM DPC micelle, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Yeast Oligosaccharyltransferase subunit Ost4	[U-13C; U-15N]		400 uM
2	DPC micelle	natural abundance		100 mM

2 3D HNCACB

Instrument

Bruker AVANCE - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Yeast Oligosaccharyltransferase subunit Ost4	[U-13C; U-15N]		400 uM
2	DPC micelle	natural abundance		100 mM

3 3D CBCA(CO)NH

Instrument

Bruker AVANCE - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Yeast Oligosaccharyltransferase subunit Ost4	[U-13C; U-15N]		400 uM
2	DPC micelle	natural abundance		100 mM

4 3D 1H-15N TOCSY

Instrument

Bruker AVANCE - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Yeast Oligosaccharyltransferase subunit Ost4	[U-13C; U-15N]		400 uM
2	DPC micelle	natural abundance		100 mM

5 3D 1H-15N NOESY

Instrument

Bruker AVANCE - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Yeast Oligosaccharyltransferase subunit Ost4	[U-13C; U-15N]		400 uM
2	DPC micelle	natural abundance		100 mM

6 3D 1H-15N NOESY

Instrument

Bruker AVANCE - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Yeast Oligosaccharyltransferase subunit Ost4	[U-13C; U-15N]		400 uM
2	DPC micelle	natural abundance		100 mM

7 3D HCCH-TOCSY

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Yeast Oligosaccharyltransferase subunit Ost4	[U-13C; U-15N]		400 uM
2	DPC micelle	natural abundance		100 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30760_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr30760_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	OK	406		91.1 (chain: 1, length: 45)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 45, Sequence coverage: 91.1%
- Total number of assignments
  - ¹H chemical shifts: 257
  - ¹³C chemical shifts: 109
  - ¹⁵N chemical shifts: 40
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	268	205	76.5
¹³C chemical shifts	211	109	51.7
¹⁵N chemical shifts	48	40	83.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	90	80	88.9
¹³C chemical shifts	90	41	45.6
¹⁵N chemical shifts	44	39	88.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	178	125	70.2
¹³C chemical shifts	121	68	56.2
¹⁵N chemical shifts	4	1	25.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	33	26	78.8
¹³C chemical shifts	33	20	60.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	23	0	0.0
¹³C chemical shifts	23	0	0.0

Keywords TRANSFERASE

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Found 1 Document (0.631 seconds)

BMRB: 30760

Sample

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. Oligosaccharyltransferase, : 1 : 4 : 17 entities Detail

Experiments performed 7 experiments Detail

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Chemical shift validation 3 contents Detail