BMRBj - BMREntryMaster

Found 1 Document (0.49 seconds)

BMRB: 30768

6XOR

Structure of the Self-Association Domain of Swallow

Authors

Loening, N.M., Barbar, E.

Assembly

Dynein intermediate chain protein

Entity

1. Dynein intermediate chain protein (polymer, Thiol state: not present), 71 monomers, 8547.366 × 2 Da Detail

SFDRLLAENE SLQQKINSLE VEAKRLQGFN EYVQERLDRI TDDFVKMKDN FETLRTELSE AQQKLRRQQD N

Total weight

17094.732 Da

Max. entity weight

8547.366 Da

Source organism

Drosophila melanogaster

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.5 %, Completeness (bb): 99.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	98.5 % (872 of 885)	97.4 % (456 of 468)	100.0 % (326 of 326)	98.9 % (90 of 91)
Backbone	99.1 % (422 of 426)	97.2 % (139 of 143)	100.0 % (212 of 212)	100.0 % (71 of 71)
Sidechain	98.3 % (520 of 529)	97.5 % (317 of 325)	100.0 % (184 of 184)	95.0 % (19 of 20)
Aromatic	97.9 % (47 of 48)	95.8 % (23 of 24)	100.0 % (24 of 24)
Methyl	100.0 % (68 of 68)	100.0 % (34 of 34)	100.0 % (34 of 34)

1. entity 1

SFDRLLAENE SLQQKINSLE VEAKRLQGFN EYVQERLDRI TDDFVKMKDN FETLRTELSE AQQKLRRQQD N

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.6, Details 0.5 mM [U-99% 13C; U-99% 15N] Residues 205-275 of Swallow, 10 mM sodium chloride, 20 mM MES, 1 mM sodium azide, 0.2 mM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	Residues 205-275 of Swallow	[U-99% 13C; U-99% 15N]	0.5 mM
2	sodium chloride	natural abundance	10 mM
3	MES	natural abundance	20 mM
4	sodium azide	natural abundance	1 mM
5	DSS	natural abundance	0.2 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.6, Details 0.4 mM [U-99% 13C; U-99% 15N] Residues 205-275 of Swallow, 10 mM sodium chloride, 20 mM MES, 1 mM sodium azide, 0.2 mM DSS, 0.4 mM unlabeled Residues 205-275 of Swallow, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
6	Residues 205-275 of Swallow	[U-99% 13C; U-99% 15N]	0.4 mM
7	sodium chloride	natural abundance	10 mM
8	MES	natural abundance	20 mM
9	sodium azide	natural abundance	1 mM
10	DSS	natural abundance	0.2 mM
11	unlabeled Residues 205-275 of Swallow	natural abundance	0.4 mM

Release date

2020-07-15

Citation

Structural characterization of the self-association domain of swallow
Loening, N.M., Barbar, E.
Protein Sci. (2021), 30, 1056-1063, PubMed 33641207 , DOI 10.1002/pro.4055 , Abstract

Related entities 1. Dynein intermediate chain protein, : 1 : 2 : 31 entities Detail

Interaction partners 1. Dynein intermediate chain protein, : 10 interactors Detail

More details for 10 interactors identified by 1 citations

Experiments performed 13 experiments Detail

1 2D 1H-13C HSQC

Instrument

Bruker AVANCE III - 950 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.6, Details 0.5 mM [U-99% 13C; U-99% 15N] Residues 205-275 of Swallow, 10 mM sodium chloride, 20 mM MES, 1 mM sodium azide, 0.2 mM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	Residues 205-275 of Swallow	[U-99% 13C; U-99% 15N]	0.5 mM
2	sodium chloride	natural abundance	10 mM
3	MES	natural abundance	20 mM
4	sodium azide	natural abundance	1 mM
5	DSS	natural abundance	0.2 mM

2 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 950 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Residues 205-275 of Swallow	[U-99% 13C; U-99% 15N]	0.5 mM
2	sodium chloride	natural abundance	10 mM
3	MES	natural abundance	20 mM
4	sodium azide	natural abundance	1 mM
5	DSS	natural abundance	0.2 mM

3 3D 1H-15N NOESY

Instrument

Bruker AVANCE III - 950 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Residues 205-275 of Swallow	[U-99% 13C; U-99% 15N]	0.5 mM
2	sodium chloride	natural abundance	10 mM
3	MES	natural abundance	20 mM
4	sodium azide	natural abundance	1 mM
5	DSS	natural abundance	0.2 mM

4 3D 1H-13C NOESY

Instrument

Bruker AVANCE III - 950 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Residues 205-275 of Swallow	[U-99% 13C; U-99% 15N]	0.5 mM
2	sodium chloride	natural abundance	10 mM
3	MES	natural abundance	20 mM
4	sodium azide	natural abundance	1 mM
5	DSS	natural abundance	0.2 mM

5 3D 1H-13C NOESY aromatic

Instrument

Bruker AVANCE III - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.6, Details 0.4 mM [U-99% 13C; U-99% 15N] Residues 205-275 of Swallow, 10 mM sodium chloride, 20 mM MES, 1 mM sodium azide, 0.2 mM DSS, 0.4 mM unlabeled Residues 205-275 of Swallow, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
6	Residues 205-275 of Swallow	[U-99% 13C; U-99% 15N]	0.4 mM
7	sodium chloride	natural abundance	10 mM
8	MES	natural abundance	20 mM
9	sodium azide	natural abundance	1 mM
10	DSS	natural abundance	0.2 mM
11	unlabeled Residues 205-275 of Swallow	natural abundance	0.4 mM

6 3D 1H-13C NOESY

Instrument

Bruker AVANCE III - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
6	Residues 205-275 of Swallow	[U-99% 13C; U-99% 15N]	0.4 mM
7	sodium chloride	natural abundance	10 mM
8	MES	natural abundance	20 mM
9	sodium azide	natural abundance	1 mM
10	DSS	natural abundance	0.2 mM
11	unlabeled Residues 205-275 of Swallow	natural abundance	0.4 mM

7 3D HNCACB

Instrument

Bruker AVANCE III - 950 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Residues 205-275 of Swallow	[U-99% 13C; U-99% 15N]	0.5 mM
2	sodium chloride	natural abundance	10 mM
3	MES	natural abundance	20 mM
4	sodium azide	natural abundance	1 mM
5	DSS	natural abundance	0.2 mM

8 3D HN(COCA)CB

Instrument

Bruker AVANCE III - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Residues 205-275 of Swallow	[U-99% 13C; U-99% 15N]	0.5 mM
2	sodium chloride	natural abundance	10 mM
3	MES	natural abundance	20 mM
4	sodium azide	natural abundance	1 mM
5	DSS	natural abundance	0.2 mM

9 3D HBHA(CO)NH

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Residues 205-275 of Swallow	[U-99% 13C; U-99% 15N]	0.5 mM
2	sodium chloride	natural abundance	10 mM
3	MES	natural abundance	20 mM
4	sodium azide	natural abundance	1 mM
5	DSS	natural abundance	0.2 mM

10 3D H(CCO)NH

Instrument

Bruker AVANCE II - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Residues 205-275 of Swallow	[U-99% 13C; U-99% 15N]	0.5 mM
2	sodium chloride	natural abundance	10 mM
3	MES	natural abundance	20 mM
4	sodium azide	natural abundance	1 mM
5	DSS	natural abundance	0.2 mM

11 3D C(CO)NH

Instrument

Bruker AVANCE II - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Residues 205-275 of Swallow	[U-99% 13C; U-99% 15N]	0.5 mM
2	sodium chloride	natural abundance	10 mM
3	MES	natural abundance	20 mM
4	sodium azide	natural abundance	1 mM
5	DSS	natural abundance	0.2 mM

12 3D HNCO

Instrument

Bruker AVANCE II - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Residues 205-275 of Swallow	[U-99% 13C; U-99% 15N]	0.5 mM
2	sodium chloride	natural abundance	10 mM
3	MES	natural abundance	20 mM
4	sodium azide	natural abundance	1 mM
5	DSS	natural abundance	0.2 mM

13 3D HN(CA)CO

Instrument

Bruker AVANCE III - 950 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Residues 205-275 of Swallow	[U-99% 13C; U-99% 15N]	0.5 mM
2	sodium chloride	natural abundance	10 mM
3	MES	natural abundance	20 mM
4	sodium azide	natural abundance	1 mM
5	DSS	natural abundance	0.2 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30768_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr30768_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	969		100.0 (chain: 1, length: 71)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1

Status: Warning

Sequence coverage of experimental data

Entity_assembly_ID: 1, Chain length: 71, Sequence coverage: 100.0%

--------10--------20--------30--------40--------50--------60--------70-
SFDRLLAENESLQQKINSLEVEAKRLQGFNEYVQERLDRITDDFVKMKDNFETLRTELSEAQQKLRRQQDN
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
SFDRLLAENESLQQKINSLEVEAKRLQGFNEYVQERLDRITDDFVKMKDNFETLRTELSEAQQKLRRQQDN

Total number of assignments
- ¹H chemical shifts: 535
- ¹³C chemical shifts: 345
- ¹⁵N chemical shifts: 89

Completeness of assignments

Entity_assemble_ID: 1

Excluded Atom_ID in statistics

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
4	ARG	CZ	159.696
9	ASN	CG	175.979
13	GLN	CD	179.717
14	GLN	CD	180.269
17	ASN	CG	176.042
25	ARG	CZ	159.786
27	GLN	CD	179.963
34	GLN	CD	180.236
36	ARG	CZ	160.123
39	ARG	CZ	159.735
50	ASN	CG	175.759
55	ARG	CZ	160.086
62	GLN	CD	179.498
63	GLN	CD	180.284
66	ARG	CZ	159.696
67	ARG	CZ	159.696
68	GLN	CD	180.284
69	GLN	CD	180.621
71	ASN	CG	178.467

All atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	468	465	99.4
¹³C chemical shifts	326	326	100.0
¹⁵N chemical shifts	91	89	97.8

Backbone atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	143	142	99.3
¹³C chemical shifts	142	142	100.0
¹⁵N chemical shifts	71	70	98.6

Side chain atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	325	323	99.4
¹³C chemical shifts	184	184	100.0
¹⁵N chemical shifts	20	19	95.0

Methyl group atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	35	35	100.0
¹³C chemical shifts	35	35	100.0

Aromatic group atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	24	24	100.0
¹³C chemical shifts	24	24	100.0

Rotameric state of ILE , LEU, and VAL
Histogram of normalized assigned chemical shifts
Random coil index and derived parameters (S² and NMR RMSD)
- Chain_ID: 1

Keywords RNA BINDING PROTEIN, coiled coil, self-association domain

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Found 1 Document (0.49 seconds)

BMRB: 30768

Sample #1

Sample #2

Related entities 1. Dynein intermediate chain protein, : 1 : 2 : 31 entities Detail

Interaction partners 1. Dynein intermediate chain protein, : 10 interactors Detail

Experiments performed 13 experiments Detail

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Chemical shift validation 3 contents Detail