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BMRB: 30773

6XRY

Intrinsically disordered bacterial polar organizing protein Z, PopZ, interacts with protein binding partners through an N-terminal Molecular Recognition Feature

Authors

Nordyke, C.T., Ahmed, Y., Puterbaugh, R.Z., Bowman, G.R., Varga, K.

Assembly

Polar organizing protein Z

Entity

1. Polar organizing protein Z (polymer, Thiol state: not present), 141 monomers, 14978.20 Da Detail

MSDQSQEPTM EEILASIRRI ISEDDAPAEP AAEAAPPPPP EPEPEPVSFD DEVLELTDPI APEPELPPLE TVGDIDVYSP PEPESEPAYT PPPAAPVFDR DEVAEQLVGV SAASAAASAF GSLSSALLMP KDGLEHHHHH H

Formula weight

14978.2 Da

Source organism

Caulobacter vibrioides CB15

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 84.4 %, Completeness: 61.9 %, Completeness (bb): 83.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	61.9 % (942 of 1521)	51.0 % (402 of 789)	70.8 % (432 of 610)	88.5 % (108 of 122)
Backbone	83.8 % (667 of 796)	81.6 % (213 of 261)	82.8 % (347 of 419)	92.2 % (107 of 116)
Sidechain	45.0 % (388 of 862)	35.8 % (189 of 528)	60.4 % (198 of 328)	16.7 % (1 of 6)
Aromatic	0.0 % (0 of 70)	0.0 % (0 of 35)	0.0 % (0 of 35)
Methyl	66.2 % (94 of 142)	60.6 % (43 of 71)	71.8 % (51 of 71)

1. entity 1

MSDQSQEPTM EEILASIRRI ISEDDAPAEP AAEAAPPPPP EPEPEPVSFD DEVLELTDPI APEPELPPLE TVGDIDVYSP PEPESEPAYT PPPAAPVFDR DEVAEQLVGV SAASAAASAF GSLSSALLMP KDGLEHHHHH H

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1.0 atm, Temperature 298 K, pH 5.5, Details 913 uM [U-99% 13C; U-99% 15N] Polar organizing protein Z, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	Polar organizing protein Z	[U-99% 13C; U-99% 15N]		913 uM
2	NaCl	natural abundance		20 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1.0 atm, Temperature 298 K, pH 5.5, Details 142 uM [U-99% 15N] Polar organizing protein Z, 5.4% acrylamide gel with 5.4 mm outer diameter (OD),95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
3	Polar organizing protein Z	[U-99% 15N]	142 uM
4	acrylamide gel with 5.4 mm outer diameter	natural abundance	5.4 %
5	NaCl	natural abundance	20 mM

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1.0 atm, Temperature 298 K, pH 5.5, Details 142 uM [U-99% 15N] Polar organizing protein Z, 5.4% acrylamide gel with 6.0 mm outer diameter, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
6	Polar organizing protein Z	[U-99% 15N]	142 uM
7	acrylamide gel with 6.0 mm outer diameter	natural abundance	5.4 %
8	NaCl	natural abundance	20 mM

Sample #4

Solvent system 95% H2O/5% D2O, Pressure 1.0 atm, Temperature 298 K, pH 5.5, Details 142 uM [U-99% 15N] Polar organizing protein Z, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
9	Polar organizing protein Z	[U-99% 15N]		142 uM
10	NaCl	natural abundance		20 mM

Release date

2020-07-17

Citation

Intrinsically Disordered Bacterial Polar Organizing Protein Z, PopZ, Interacts with Protein Binding Partners Through an N-terminal Molecular Recognition Feature
Nordyke, C.T., Ahmed, Y., Puterbaugh, R.Z., Bowman, G.R., Varga, K.
J. Mol. Biol. (2020), 432, 6092-6107, PubMed 33058876 , DOI 10.1016/j.jmb.2020.09.020 , Abstract

Related entities 1. Polar organizing protein Z, : 1 entities Detail

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC