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Intrinsically disordered bacterial polar organizing protein Z, PopZ, interacts with protein binding partners through an N-terminal Molecular Recognition Feature
Authors
Nordyke, C.T., Ahmed, Y., Puterbaugh, R.Z., Bowman, G.R., Varga, K.
Assembly
Polar organizing protein Z
Entity
1. Polar organizing protein Z (polymer, Thiol state: not present), 141 monomers, 14978.20 Da Detail

MSDQSQEPTM EEILASIRRI ISEDDAPAEP AAEAAPPPPP EPEPEPVSFD DEVLELTDPI APEPELPPLE TVGDIDVYSP PEPESEPAYT PPPAAPVFDR DEVAEQLVGV SAASAAASAF GSLSSALLMP KDGLEHHHHH H


Formula weight
14978.2 Da
Source organism
Caulobacter vibrioides CB15
Exptl. method
solution NMR
Data set
assigned_chemical_shifts, spectral_peak_list
Chem. Shift Complete
Sequence coverage: 84.4 %, Completeness: 61.9 %, Completeness (bb): 83.8 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All61.9 % (942 of 1521)51.0 % (402 of 789)70.8 % (432 of 610)88.5 % (108 of 122)
Backbone83.8 % (667 of 796)81.6 % (213 of 261)82.8 % (347 of 419)92.2 % (107 of 116)
Sidechain45.0 % (388 of 862)35.8 % (189 of 528)60.4 % (198 of 328)16.7 % (1 of 6)
Aromatic 0.0 % (0 of 70) 0.0 % (0 of 35) 0.0 % (0 of 35)
Methyl66.2 % (94 of 142)60.6 % (43 of 71)71.8 % (51 of 71)

1. entity 1

MSDQSQEPTM EEILASIRRI ISEDDAPAEP AAEAAPPPPP EPEPEPVSFD DEVLELTDPI APEPELPPLE TVGDIDVYSP PEPESEPAYT PPPAAPVFDR DEVAEQLVGV SAASAAASAF GSLSSALLMP KDGLEHHHHH H

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1.0 atm, Temperature 298 K, pH 5.5, Details 913 uM [U-99% 13C; U-99% 15N] Polar organizing protein Z, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
1Polar organizing protein Z[U-99% 13C; U-99% 15N]913 uM
2NaClnatural abundance20 mM
Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1.0 atm, Temperature 298 K, pH 5.5, Details 142 uM [U-99% 15N] Polar organizing protein Z, 5.4% acrylamide gel with 5.4 mm outer diameter (OD),95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
3Polar organizing protein Z[U-99% 15N]142 uM
4acrylamide gel with 5.4 mm outer diameternatural abundance5.4 %
5NaClnatural abundance20 mM
Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1.0 atm, Temperature 298 K, pH 5.5, Details 142 uM [U-99% 15N] Polar organizing protein Z, 5.4% acrylamide gel with 6.0 mm outer diameter, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
6Polar organizing protein Z[U-99% 15N]142 uM
7acrylamide gel with 6.0 mm outer diameternatural abundance5.4 %
8NaClnatural abundance20 mM
Sample #4

Solvent system 95% H2O/5% D2O, Pressure 1.0 atm, Temperature 298 K, pH 5.5, Details 142 uM [U-99% 15N] Polar organizing protein Z, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
9Polar organizing protein Z[U-99% 15N]142 uM
10NaClnatural abundance20 mM

Release date
2020-07-17
Citation
Intrinsically Disordered Bacterial Polar Organizing Protein Z, PopZ, Interacts with Protein Binding Partners Through an N-terminal Molecular Recognition Feature
Nordyke, C.T., Ahmed, Y., Puterbaugh, R.Z., Bowman, G.R., Varga, K.
J. Mol. Biol. (2020), 432, 6092-6107, PubMed 33058876 , DOI 10.1016/j.jmb.2020.09.020 ,
Related entities 1. Polar organizing protein Z, : 1 entities Detail
Experiments performed 16 experiments Detail
Chemical shift validation 3 contents Detail
Keywords MoRF, Molecular Recognition Feature, PROTEIN BINDING, hub protein