BMRB: 30779

NMR structure of the Streptococcus pyogenes NAD+-glycohydrolase translocation domain

Authors

Velarde, J.J., Piai, A., Chou, J.J., Wessels, M.

Assembly

ADP-ribosyl cyclase / cyclic ADP-ribose hydrolase

Entity

1. ADP-ribosyl cyclase / cyclic ADP-ribose hydrolase (polymer, Thiol state: not present), 159 monomers, 17773.75 Da Detail

GPVSGKENKK SDVKYETTKV MEANATSSKE DNHVMHTLDG SMSTVWEENS PGGGVGEVLS YKFASPMHIG RILIVNGDTS SKENYYKKNR IAKADVKYYN GNKLVLFQKI ELGDTYTKKP HHIEIDKKLD VDRIDIEVTE VHQGQNKDIL ALSEVTFGN

Formula weight

17773.75 Da

Source organism

Streptococcus pyogenes

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.0 %, Completeness: 44.6 %, Completeness (bb): 84.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	44.6 % (825 of 1849)	28.3 % (272 of 962)	57.4 % (410 of 714)	82.7 % (143 of 173)
Backbone	84.6 % (800 of 946)	77.4 % (253 of 327)	87.3 % (405 of 464)	91.6 % (142 of 155)
Sidechain	13.3 % (139 of 1049)	3.0 % (19 of 635)	30.1 % (119 of 396)	5.6 % (1 of 18)
Aromatic	4.9 % (6 of 122)	6.6 % (4 of 61)	3.3 % (2 of 60)	0.0 % (0 of 1)
Methyl	5.5 % (9 of 164)	2.4 % (2 of 82)	8.5 % (7 of 82)

1. entity 1

GPVSGKENKK SDVKYETTKV MEANATSSKE DNHVMHTLDG SMSTVWEENS PGGGVGEVLS YKFASPMHIG RILIVNGDTS SKENYYKKNR IAKADVKYYN GNKLVLFQKI ELGDTYTKKP HHIEIDKKLD VDRIDIEVTE VHQGQNKDIL ALSEVTFGN

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 null, Temperature 303 K, pH 5.5, Details 1 mM [U-13C; U-15N] NADase translocation domain, 20mM MES, 50mM NaCl, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	NADase translocation domain	[U-13C; U-15N]		1 mM
2	MES	natural abundance		20 mM
3	NaCl	natural abundance		50 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 null, Temperature 303 K, pH 5.5, Details 0.56 mM [U-13C; U-15N; U-2H] NADase translocation domain, 20mM MES, 80mM NaCl, 0.5mM EDTA, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
4	NADase translocation domain	[U-13C; U-15N; U-2H]		0.56 mM
5	MES	natural abundance		20 mM
6	NaCl	natural abundance		80 mM
7	EDTA	natural abundance		0.5 mM

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Release date

2020-07-28

Citation

Structure of the Streptococcus pyogenes NAD⁺ Glycohydrolase Translocation Domain and Its Essential Role in Toxin Binding to Oropharyngeal Keratinocytes
Velarde, J.J., Piai, A., Lichtenstein, I.J., Lynskey, N.N., Chou, J.J., Wessels, M.R.
J. Bacteriol. (2022), 204, e0036621-e0036621, PubMed 34694903 , DOI 10.1128/JB.00366-21 , Abstract

Related entities 1. ADP-ribosyl cyclase / cyclic ADP-ribose hydrolase, : 1 entities Detail

Experiments performed 8 experiments Detail

Chemical shift validation 3 contents Detail