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BMRB: 30790

7JQ8

Solution NMR structure of human Brd3 ET complexed with NSD3(148-184) peptide

Authors

Aiyer, S., Swapna, G.V.T., Roth, M.J., Montelione, G.T.

Assembly

Bromodomain-containing protein 3, Histone-lysine N-methyltransferase NSD3 (E.C.2.1.1.-)

Entity

1. Bromodomain-containing protein 3, Histone-lysine N-methyltransferase NSD3 (E.C.2.1.1.-), entity 1 (polymer), 96 monomers, 11242.36 Da Detail

HHHHHHSHMG KQASASYDSE EEEEGLPMSY DEKRQLSLDI NRLPGEKLGR VVHIIQSREP SLRDSNPDEI EIDFETLKPT TLRELERYVK SCLQKK

2. Bromodomain-containing protein 3, Histone-lysine N-methyltransferase NSD3 (E.C.2.1.1.-), entity 2 (polymer), 39 monomers, 4325.802 Da Detail

EFTGSPEIKL KITKTIQNGR ELFESSLCGD LLNEVQASE

Total weight

15568.162 Da

Max. entity weight

11242.36 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 94.8 %, Completeness: 77.9 %, Completeness (bb): 76.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	77.9 % (1245 of 1598)	83.7 % (703 of 840)	67.9 % (415 of 611)	86.4 % (127 of 147)
Backbone	76.7 % (612 of 798)	90.4 % (245 of 271)	63.1 % (251 of 398)	89.9 % (116 of 129)
Sidechain	81.0 % (752 of 928)	80.5 % (458 of 569)	83.0 % (283 of 341)	61.1 % (11 of 18)
Aromatic	51.2 % (44 of 86)	51.2 % (22 of 43)	51.2 % (22 of 43)
Methyl	99.2 % (125 of 126)	98.4 % (62 of 63)	100.0 % (63 of 63)

1. entity 1

HHHHHHSHMG KQASASYDSE EEEEGLPMSY DEKRQLSLDI NRLPGEKLGR VVHIIQSREP SLRDSNPDEI EIDFETLKPT TLRELERYVK SCLQKK

2. entity 2

EFTGSPEIKL KITKTIQNGR ELFESSLCGD LLNEVQASE

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.05) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 0.5 mM [U-100% 13C; U-100% 15N] NSD3(148-184), 100 mM sodium chloride, 20 mM sodium phosphate, 2 mM 2-mercaptoethanol, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	NSD3(148-184)	[U-100% 13C; U-100% 15N]	0.5 (±0.02) mM
2	sodium chloride	natural abundance	100 mM
3	sodium phosphate	natural abundance	20 mM
4	2-mercaptoethanol	natural abundance	2 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.05) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 0.25 mM NSD3(148-184), 0.25 mM U-100% 13C; U-100% 15 Brd3ET, 100 mM sodium chloride, 20 mM sodium phosphate, 2 mM 2-mercaptoethanol, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	NSD3(148-184)	natural abundance	0.25 (±0.02) mM
6	sodium chloride	natural abundance	100 (±0.02) mM
7	sodium phosphate	natural abundance	20 (±0.02) mM
8	2-mercaptoethanol	natural abundance	2 (±0.02) mM
9	Brd3ET	U-100% 13C; U-100% 15	0.25 (±0.02) mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.05) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 0.25 mM [U-100% 13C; U-100% 15N] NSD3(148-184), 0.2 mM [U-100% 13C; U-100% 15N] Brd3ET, 100 mM sodium chloride, 20 mM sodium phosphate, 2 mM 2-mercaptoethanol, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
10	NSD3(148-184)	[U-100% 13C; U-100% 15N]	0.25 (±0.02) mM
11	sodium chloride	natural abundance	100 (±0.02) mM
12	sodium phosphate	natural abundance	20 (±0.02) mM
13	2-mercaptoethanol	natural abundance	2 (±0.02) mM
14	Brd3ET	[U-100% 13C; U-100% 15N]	0.2 (±0.02) mM

Release date

2020-10-15

Citation

A common binding motif in the ET domain of BRD3 forms polymorphic structural interfaces with host and viral proteins
Aiyer, S., Swapna, G.V.T., Ma, L., Liu, G., Hao, J., Chalmers, G., Jacobs, B.C., Montelione, G.T., Roth, M.
Structure (2021), 29, 886-898, PubMed 33592170 , DOI 10.1016/j.str.2021.01.010 , Abstract

Entries sharing articles BMRB: 4 entries Detail

  BMRB: 30791 released on 2020-11-01
    Title Solution NMR structure and dynamics of human Brd3 ET in complex with MLV IN CTD
  BMRB: 30786 released on 2020-09-25
    Title Solution NMR structure of human Brd3 ET domain with MLV IN C-terminal Tail Peptide (TP) complex
  BMRB: 30782 released on 2020-08-18
    Title A common binding motif in the ET domain of BRD3 participates in polymorphic structural interfaces with host and viral proteins
  BMRB: 30444 released on 2018-04-10
    Title Solution structure of the Extraterminal (ET) Domain of BRD2

Related entities 1. Bromodomain-containing protein 3, Histone-lysine N-methyltransferase NSD3 (E.C.2.1.1.-), entity 1, : 1 : 3 : 7 : 42 entities Detail

Related entities 2. Bromodomain-containing protein 3, Histone-lysine N-methyltransferase NSD3 (E.C.2.1.1.-), entity 2, : 1 : 5 : 8 entities Detail

Interaction partners 1. Bromodomain-containing protein 3, Histone-lysine N-methyltransferase NSD3 (E.C.2.1.1.-), entity 1, : 6 interactors Detail

More details for 6 interactors identified by 1 citations

Interaction partners 2. Bromodomain-containing protein 3, Histone-lysine N-methyltransferase NSD3 (E.C.2.1.1.-), entity 2, : 36 interactors Detail

More details for 36 interactors identified by 6 citations

Experiments performed 32 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE III HD - 600 MHz Equipped with 5mm TCI probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 (±0.05) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 0.5 mM [U-100% 13C; U-100% 15N] NSD3(148-184), 100 mM sodium chloride, 20 mM sodium phosphate, 2 mM 2-mercaptoethanol, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	NSD3(148-184)	[U-100% 13C; U-100% 15N]	0.5 (±0.02) mM
2	sodium chloride	natural abundance	100 mM
3	sodium phosphate	natural abundance	20 mM
4	2-mercaptoethanol	natural abundance	2 mM

2 2D 1H-13C HSQC

Instrument

Bruker AVANCE III HD - 600 MHz Equipped with 5mm TCI probe

Sample

#	Name	Isotope labeling	Concentration
1	NSD3(148-184)	[U-100% 13C; U-100% 15N]	0.5 (±0.02) mM
2	sodium chloride	natural abundance	100 mM
3	sodium phosphate	natural abundance	20 mM
4	2-mercaptoethanol	natural abundance	2 mM

3 2D HNOE

Instrument

Bruker AVANCE III HD - 600 MHz Equipped with 5mm TCI probe

Sample

#	Name	Isotope labeling	Concentration
1	NSD3(148-184)	[U-100% 13C; U-100% 15N]	0.5 (±0.02) mM
2	sodium chloride	natural abundance	100 mM
3	sodium phosphate	natural abundance	20 mM
4	2-mercaptoethanol	natural abundance	2 mM

4 3D HNCO

Instrument

Bruker AVANCE III HD - 600 MHz Equipped with 5mm TCI probe

Sample

#	Name	Isotope labeling	Concentration
1	NSD3(148-184)	[U-100% 13C; U-100% 15N]	0.5 (±0.02) mM
2	sodium chloride	natural abundance	100 mM
3	sodium phosphate	natural abundance	20 mM
4	2-mercaptoethanol	natural abundance	2 mM

5 3D HNCA

Instrument

Bruker AVANCE III HD - 600 MHz Equipped with 5mm TCI probe

Sample

#	Name	Isotope labeling	Concentration
1	NSD3(148-184)	[U-100% 13C; U-100% 15N]	0.5 (±0.02) mM
2	sodium chloride	natural abundance	100 mM
3	sodium phosphate	natural abundance	20 mM
4	2-mercaptoethanol	natural abundance	2 mM

6 3D HNCACB

Instrument

Bruker AVANCE III HD - 600 MHz Equipped with 5mm TCI probe

Sample

#	Name	Isotope labeling	Concentration
1	NSD3(148-184)	[U-100% 13C; U-100% 15N]	0.5 (±0.02) mM
2	sodium chloride	natural abundance	100 mM
3	sodium phosphate	natural abundance	20 mM
4	2-mercaptoethanol	natural abundance	2 mM

7 3D 13C_15N_simNOESY

Instrument

Bruker AVANCE III HD - 600 MHz Equipped with 5mm TCI probe

Sample

#	Name	Isotope labeling	Concentration
1	NSD3(148-184)	[U-100% 13C; U-100% 15N]	0.5 (±0.02) mM
2	sodium chloride	natural abundance	100 mM
3	sodium phosphate	natural abundance	20 mM
4	2-mercaptoethanol	natural abundance	2 mM

8 3D HCCH-TOCSY

Instrument

Bruker AVANCE III HD - 600 MHz Equipped with 5mm TCI probe

Sample

#	Name	Isotope labeling	Concentration
1	NSD3(148-184)	[U-100% 13C; U-100% 15N]	0.5 (±0.02) mM
2	sodium chloride	natural abundance	100 mM
3	sodium phosphate	natural abundance	20 mM
4	2-mercaptoethanol	natural abundance	2 mM

9 3D HBHA(CO)NH

Instrument

Bruker AVANCE III HD - 600 MHz Equipped with 5mm TCI probe

Sample

#	Name	Isotope labeling	Concentration
1	NSD3(148-184)	[U-100% 13C; U-100% 15N]	0.5 (±0.02) mM
2	sodium chloride	natural abundance	100 mM
3	sodium phosphate	natural abundance	20 mM
4	2-mercaptoethanol	natural abundance	2 mM

10 3D CBCA(CO)NH

Instrument

Bruker AVANCE III HD - 600 MHz Equipped with 5mm TCI probe

Sample

#	Name	Isotope labeling	Concentration
1	NSD3(148-184)	[U-100% 13C; U-100% 15N]	0.5 (±0.02) mM
2	sodium chloride	natural abundance	100 mM
3	sodium phosphate	natural abundance	20 mM
4	2-mercaptoethanol	natural abundance	2 mM

11 2D 1H-15N HSQC

Instrument

Bruker AVANCE - 800 MHz equipped with 5mm TXI cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 (±0.05) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 0.25 mM NSD3(148-184), 0.25 mM U-100% 13C; U-100% 15 Brd3ET, 100 mM sodium chloride, 20 mM sodium phosphate, 2 mM 2-mercaptoethanol, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	NSD3(148-184)	natural abundance	0.25 (±0.02) mM
6	sodium chloride	natural abundance	100 (±0.02) mM
7	sodium phosphate	natural abundance	20 (±0.02) mM
8	2-mercaptoethanol	natural abundance	2 (±0.02) mM
9	Brd3ET	U-100% 13C; U-100% 15	0.25 (±0.02) mM

12 3D HCCH-TOCSY

Instrument

Bruker AVANCE III HD - 600 MHz Equipped with 5mm TCI probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 (±0.05) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 0.25 mM [U-100% 13C; U-100% 15N] NSD3(148-184), 0.2 mM [U-100% 13C; U-100% 15N] Brd3ET, 100 mM sodium chloride, 20 mM sodium phosphate, 2 mM 2-mercaptoethanol, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
10	NSD3(148-184)	[U-100% 13C; U-100% 15N]	0.25 (±0.02) mM
11	sodium chloride	natural abundance	100 (±0.02) mM
12	sodium phosphate	natural abundance	20 (±0.02) mM
13	2-mercaptoethanol	natural abundance	2 (±0.02) mM
14	Brd3ET	[U-100% 13C; U-100% 15N]	0.2 (±0.02) mM

13 3D HBHA(CO)NH

Instrument

Bruker AVANCE III HD - 600 MHz Equipped with 5mm TCI probe

Sample

#	Name	Isotope labeling	Concentration
10	NSD3(148-184)	[U-100% 13C; U-100% 15N]	0.25 (±0.02) mM
11	sodium chloride	natural abundance	100 (±0.02) mM
12	sodium phosphate	natural abundance	20 (±0.02) mM
13	2-mercaptoethanol	natural abundance	2 (±0.02) mM
14	Brd3ET	[U-100% 13C; U-100% 15N]	0.2 (±0.02) mM

14 3D CBCA(CO)NH

Instrument

Bruker AVANCE III HD - 600 MHz Equipped with 5mm TCI probe

Sample

#	Name	Isotope labeling	Concentration
10	NSD3(148-184)	[U-100% 13C; U-100% 15N]	0.25 (±0.02) mM
11	sodium chloride	natural abundance	100 (±0.02) mM
12	sodium phosphate	natural abundance	20 (±0.02) mM
13	2-mercaptoethanol	natural abundance	2 (±0.02) mM
14	Brd3ET	[U-100% 13C; U-100% 15N]	0.2 (±0.02) mM

15 3D HNCACB

Instrument

Bruker AVANCE III HD - 600 MHz Equipped with 5mm TCI probe

Sample

#	Name	Isotope labeling	Concentration
10	NSD3(148-184)	[U-100% 13C; U-100% 15N]	0.25 (±0.02) mM
11	sodium chloride	natural abundance	100 (±0.02) mM
12	sodium phosphate	natural abundance	20 (±0.02) mM
13	2-mercaptoethanol	natural abundance	2 (±0.02) mM
14	Brd3ET	[U-100% 13C; U-100% 15N]	0.2 (±0.02) mM

16 3D HNCA

Instrument

Bruker AVANCE III HD - 600 MHz Equipped with 5mm TCI probe

Sample

#	Name	Isotope labeling	Concentration
10	NSD3(148-184)	[U-100% 13C; U-100% 15N]	0.25 (±0.02) mM
11	sodium chloride	natural abundance	100 (±0.02) mM
12	sodium phosphate	natural abundance	20 (±0.02) mM
13	2-mercaptoethanol	natural abundance	2 (±0.02) mM
14	Brd3ET	[U-100% 13C; U-100% 15N]	0.2 (±0.02) mM

17 3D HNCO

Instrument

Bruker AVANCE III HD - 600 MHz Equipped with 5mm TCI probe

Sample

#	Name	Isotope labeling	Concentration
10	NSD3(148-184)	[U-100% 13C; U-100% 15N]	0.25 (±0.02) mM
11	sodium chloride	natural abundance	100 (±0.02) mM
12	sodium phosphate	natural abundance	20 (±0.02) mM
13	2-mercaptoethanol	natural abundance	2 (±0.02) mM
14	Brd3ET	[U-100% 13C; U-100% 15N]	0.2 (±0.02) mM

18 2D HNOE

Instrument

Bruker AVANCE III HD - 600 MHz Equipped with 5mm TCI probe

Sample

#	Name	Isotope labeling	Concentration
10	NSD3(148-184)	[U-100% 13C; U-100% 15N]	0.25 (±0.02) mM
11	sodium chloride	natural abundance	100 (±0.02) mM
12	sodium phosphate	natural abundance	20 (±0.02) mM
13	2-mercaptoethanol	natural abundance	2 (±0.02) mM
14	Brd3ET	[U-100% 13C; U-100% 15N]	0.2 (±0.02) mM

19 2D 1H-13C HSQC

Instrument

Bruker AVANCE III HD - 600 MHz Equipped with 5mm TCI probe

Sample

#	Name	Isotope labeling	Concentration
10	NSD3(148-184)	[U-100% 13C; U-100% 15N]	0.25 (±0.02) mM
11	sodium chloride	natural abundance	100 (±0.02) mM
12	sodium phosphate	natural abundance	20 (±0.02) mM
13	2-mercaptoethanol	natural abundance	2 (±0.02) mM
14	Brd3ET	[U-100% 13C; U-100% 15N]	0.2 (±0.02) mM

20 2D 1H-15N HSQC

Instrument

Bruker AVANCE III HD - 600 MHz Equipped with 5mm TCI probe

Sample

#	Name	Isotope labeling	Concentration
10	NSD3(148-184)	[U-100% 13C; U-100% 15N]	0.25 (±0.02) mM
11	sodium chloride	natural abundance	100 (±0.02) mM
12	sodium phosphate	natural abundance	20 (±0.02) mM
13	2-mercaptoethanol	natural abundance	2 (±0.02) mM
14	Brd3ET	[U-100% 13C; U-100% 15N]	0.2 (±0.02) mM

21 3D X-filtered_NOESY

Instrument

Bruker AVANCE - 800 MHz equipped with 5mm TXI cryoprobe

Sample

#	Name	Isotope labeling	Concentration
5	NSD3(148-184)	natural abundance	0.25 (±0.02) mM
6	sodium chloride	natural abundance	100 (±0.02) mM
7	sodium phosphate	natural abundance	20 (±0.02) mM
8	2-mercaptoethanol	natural abundance	2 (±0.02) mM
9	Brd3ET	U-100% 13C; U-100% 15	0.25 (±0.02) mM

22 3D 13C_edited_NOESY_aromatic

Instrument

Bruker AVANCE - 800 MHz equipped with 5mm TXI cryoprobe

Sample

#	Name	Isotope labeling	Concentration
5	NSD3(148-184)	natural abundance	0.25 (±0.02) mM
6	sodium chloride	natural abundance	100 (±0.02) mM
7	sodium phosphate	natural abundance	20 (±0.02) mM
8	2-mercaptoethanol	natural abundance	2 (±0.02) mM
9	Brd3ET	U-100% 13C; U-100% 15	0.25 (±0.02) mM

23 3D 13C_15N_simNOESY

Instrument

Bruker AVANCE - 800 MHz equipped with 5mm TXI cryoprobe

Sample

#	Name	Isotope labeling	Concentration
5	NSD3(148-184)	natural abundance	0.25 (±0.02) mM
6	sodium chloride	natural abundance	100 (±0.02) mM
7	sodium phosphate	natural abundance	20 (±0.02) mM
8	2-mercaptoethanol	natural abundance	2 (±0.02) mM
9	Brd3ET	U-100% 13C; U-100% 15	0.25 (±0.02) mM

24 3D HCCH-TOCSY

Instrument

Bruker AVANCE - 800 MHz equipped with 5mm TXI cryoprobe

Sample

#	Name	Isotope labeling	Concentration
5	NSD3(148-184)	natural abundance	0.25 (±0.02) mM
6	sodium chloride	natural abundance	100 (±0.02) mM
7	sodium phosphate	natural abundance	20 (±0.02) mM
8	2-mercaptoethanol	natural abundance	2 (±0.02) mM
9	Brd3ET	U-100% 13C; U-100% 15	0.25 (±0.02) mM

25 3D CBCA(CO)NH

Instrument

Bruker AVANCE - 800 MHz equipped with 5mm TXI cryoprobe

Sample

#	Name	Isotope labeling	Concentration
5	NSD3(148-184)	natural abundance	0.25 (±0.02) mM
6	sodium chloride	natural abundance	100 (±0.02) mM
7	sodium phosphate	natural abundance	20 (±0.02) mM
8	2-mercaptoethanol	natural abundance	2 (±0.02) mM
9	Brd3ET	U-100% 13C; U-100% 15	0.25 (±0.02) mM

26 3D HNCACB

Instrument

Bruker AVANCE - 800 MHz equipped with 5mm TXI cryoprobe

Sample

#	Name	Isotope labeling	Concentration
5	NSD3(148-184)	natural abundance	0.25 (±0.02) mM
6	sodium chloride	natural abundance	100 (±0.02) mM
7	sodium phosphate	natural abundance	20 (±0.02) mM
8	2-mercaptoethanol	natural abundance	2 (±0.02) mM
9	Brd3ET	U-100% 13C; U-100% 15	0.25 (±0.02) mM

27 3D HBHA(CO)NH

Instrument

Bruker AVANCE - 800 MHz equipped with 5mm TXI cryoprobe

Sample

#	Name	Isotope labeling	Concentration
5	NSD3(148-184)	natural abundance	0.25 (±0.02) mM
6	sodium chloride	natural abundance	100 (±0.02) mM
7	sodium phosphate	natural abundance	20 (±0.02) mM
8	2-mercaptoethanol	natural abundance	2 (±0.02) mM
9	Brd3ET	U-100% 13C; U-100% 15	0.25 (±0.02) mM

28 3D HNCO

Instrument

Bruker AVANCE - 800 MHz equipped with 5mm TXI cryoprobe

Sample

#	Name	Isotope labeling	Concentration
5	NSD3(148-184)	natural abundance	0.25 (±0.02) mM
6	sodium chloride	natural abundance	100 (±0.02) mM
7	sodium phosphate	natural abundance	20 (±0.02) mM
8	2-mercaptoethanol	natural abundance	2 (±0.02) mM
9	Brd3ET	U-100% 13C; U-100% 15	0.25 (±0.02) mM

29 2D HNOE

Instrument

Bruker AVANCE - 800 MHz equipped with 5mm TXI cryoprobe

Sample

#	Name	Isotope labeling	Concentration
5	NSD3(148-184)	natural abundance	0.25 (±0.02) mM
6	sodium chloride	natural abundance	100 (±0.02) mM
7	sodium phosphate	natural abundance	20 (±0.02) mM
8	2-mercaptoethanol	natural abundance	2 (±0.02) mM
9	Brd3ET	U-100% 13C; U-100% 15	0.25 (±0.02) mM

30 2D 1H-13C HSQC aliphatic

Instrument

Bruker AVANCE - 800 MHz equipped with 5mm TXI cryoprobe

Sample

#	Name	Isotope labeling	Concentration
5	NSD3(148-184)	natural abundance	0.25 (±0.02) mM
6	sodium chloride	natural abundance	100 (±0.02) mM
7	sodium phosphate	natural abundance	20 (±0.02) mM
8	2-mercaptoethanol	natural abundance	2 (±0.02) mM
9	Brd3ET	U-100% 13C; U-100% 15	0.25 (±0.02) mM

31 3D 13C_15N_simNOESY

Instrument

Bruker AVANCE III HD - 600 MHz Equipped with 5mm TCI probe

Sample

#	Name	Isotope labeling	Concentration
10	NSD3(148-184)	[U-100% 13C; U-100% 15N]	0.25 (±0.02) mM
11	sodium chloride	natural abundance	100 (±0.02) mM
12	sodium phosphate	natural abundance	20 (±0.02) mM
13	2-mercaptoethanol	natural abundance	2 (±0.02) mM
14	Brd3ET	[U-100% 13C; U-100% 15N]	0.2 (±0.02) mM

32 3D 13C_edited_NOESY_aromatic

Instrument

Bruker AVANCE III HD - 600 MHz Equipped with 5mm TCI probe

Sample

#	Name	Isotope labeling	Concentration
10	NSD3(148-184)	[U-100% 13C; U-100% 15N]	0.25 (±0.02) mM
11	sodium chloride	natural abundance	100 (±0.02) mM
12	sodium phosphate	natural abundance	20 (±0.02) mM
13	2-mercaptoethanol	natural abundance	2 (±0.02) mM
14	Brd3ET	[U-100% 13C; U-100% 15N]	0.2 (±0.02) mM

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30790_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr30790_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1368		91.7 (chain: 1, length: 96), 100.0 (chain: 2, length: 39)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 96, Sequence coverage: 91.7%
  - Entity_assembly_ID: 2, Chain length: 39, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 837
  - ¹³C chemical shifts: 408
  - ¹⁵N chemical shifts: 123
- Completeness of assignments
  - Entity_assemble_ID: 1
  - Entity_assemble_ID: 2
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
  - Chain_ID: 2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	603	515	85.4
¹³C chemical shifts	437	289	66.1
¹⁵N chemical shifts	104	88	84.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	191	174	91.1
¹³C chemical shifts	192	88	45.8
¹⁵N chemical shifts	91	82	90.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	412	341	82.8
¹³C chemical shifts	245	201	82.0
¹⁵N chemical shifts	13	6	46.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	43	43	100.0
¹³C chemical shifts	43	43	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	33	17	51.5
¹³C chemical shifts	33	17	51.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	237	194	81.9
¹³C chemical shifts	174	119	68.4
¹⁵N chemical shifts	43	35	81.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	80	72	90.0
¹³C chemical shifts	78	39	50.0
¹⁵N chemical shifts	38	32	84.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	157	122	77.7
¹³C chemical shifts	96	80	83.3
¹⁵N chemical shifts	5	3	60.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	22	21	95.5
¹³C chemical shifts	22	20	90.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	10	5	50.0
¹³C chemical shifts	10	5	50.0

Keywords Brd3 ET, Integrase, NSD3 solution NMR, SIGNALING PROTEIN, extra terminal domain

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Found 1 Document (3.297 seconds)

BMRB: 30790

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Entries sharing articles BMRB: 4 entries Detail

Related entities 1. Bromodomain-containing protein 3, Histone-lysine N-methyltransferase NSD3 (E.C.2.1.1.-), entity 1, : 1 : 3 : 7 : 42 entities Detail

Related entities 2. Bromodomain-containing protein 3, Histone-lysine N-methyltransferase NSD3 (E.C.2.1.1.-), entity 2, : 1 : 5 : 8 entities Detail

Interaction partners 1. Bromodomain-containing protein 3, Histone-lysine N-methyltransferase NSD3 (E.C.2.1.1.-), entity 1, : 6 interactors Detail

Interaction partners 2. Bromodomain-containing protein 3, Histone-lysine N-methyltransferase NSD3 (E.C.2.1.1.-), entity 2, : 36 interactors Detail

Experiments performed 32 experiments Detail

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