BMRBj - BMREntryMaster

Found 1 Document (0.36 seconds)

BMRB: 30797

7K4L

Dengue 5'UTR SLA

Authors

Sun, Y.T.

Assembly

DenvSLA RNA

Entity

1. DenvSLA RNA (polymer, Thiol state: not present), 36 monomers, 11584.77 Da Detail

GGAGUGGUUA GUCUACGUUU CGACGUAGUU CUAACC

Formula weight

11584.77 Da

Source organism

Escherichia phage T7

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.9 %, Completeness: 30.3 %, Completeness (bb): 20.4 % Detail

Polymer type: polyribonucleotide

	Total	¹H
All	30.3 % (95 of 314)	30.3 % (95 of 314)
Suger, PO4	20.4 % (44 of 216)	20.4 % (44 of 216)
Nucleobase	52.0 % (51 of 98)	52.0 % (51 of 98)
Aromatic	60.7 % (51 of 84)	60.7 % (51 of 84)

1. entity 1

GGAGUGGUUA GUCUACGUUU CGACGUAGUU CUAACC

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 200 uM DenvSLA, 10 mM potassium phosphate, 10 mM potassium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	DenvSLA	natural abundance	200 uM
2	potassium phosphate	natural abundance	10 mM
3	potassium chloride	natural abundance	10 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 200 uM [U-100% 2H] DenvSLA, 10 mM potassium phosphate, 10 mM potassium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
4	DenvSLA	[U-100% 2H]	200 uM
5	potassium phosphate	natural abundance	10 mM
6	potassium chloride	natural abundance	10 mM

Release date

2020-09-28

Citation

Solution NMR Structure of Denv1 5'UTR SLA region
Sun, Y.T.

Related entities 1. DenvSLA RNA, : 1 entities Detail

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30797_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr30797_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	126		88.9 (chain: 1, length: 36)
1	Chemical shift references	chem_shift_reference_1	OK	1		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1

Status: Warning

Sequence coverage of experimental data

Entity_assembly_ID: 1, Chain length: 36, Sequence coverage: 88.9%

--------10--------20--------30------
GGAGUGGUUAGUCUACGUUUCGACGUAGUUCUAACC
    ||||||||||||||||||||||||||||||||
....UGGUUAGUCUACGUUUCGACGUAGUUCUAACC

Total number of assignments
- ¹H chemical shifts: 126

Completeness of assignments

Entity_assemble_ID: 1

Excluded Atom_ID in statistics

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
5	U	HO2'	4.375
6	G	HO2'	4.912
7	G	HO2'	4.571
8	U	HO2'	4.495
9	U	HO2'	4.467
10	A	HO2'	4.68
11	G	HO2'	4.485
12	U	HO2'	4.263
13	C	HO2'	4.264
14	U	HO2'	4.537
15	A	HO2'	4.466
16	C	HO2'	4.358
17	G	HO2'	4.477
18	U	HO2'	4.512
19	U	HO2'	3.781
20	U	HO2'	4.681
21	C	HO2'	4.082
22	G	HO2'	4.862
23	A	HO2'	4.51
24	C	HO2'	4.497
25	G	HO2'	4.516
26	U	HO2'	4.608
27	A	HO2'	4.534
28	G	HO2'	4.279
29	U	HO2'	4.381
30	U	HO2'	4.519
31	C	HO2'	4.424
32	U	HO2'	4.633
33	A	HO2'	4.532
34	A	HO2'	4.43
35	C	HO2'	4.06

All atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	314	95	30.3

Backbone atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	216	44	20.4

Side chain atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	98	51	52.0

Methyl group atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)

Aromatic group atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	24	13	54.2

Histogram of normalized assigned chemical shifts

Keywords 5'-UTR, Denv1, RNA

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Found 1 Document (0.36 seconds)

BMRB: 30797

Sample #1

Sample #2

Related entities 1. DenvSLA RNA, : 1 entities Detail

Experiments performed 4 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail