BMRB: 30817

Ax1 Domain of VEGF Readthrough Element

Authors

D'Souza, V.M., Wagner, N.O., Edwards, J.M.

Assembly

VEGF Ax1

Entity

1. VEGF Ax1 (polymer, Thiol state: not present), 16 monomers, 5278.171 Da Detail

GGAGGAAGGA GCCUCC

Formula weight

5278.171 Da

Source organism

Bos taurus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 55.8 %, Completeness (bb): 40.3 % Detail

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Polymer type: polyribonucleotide

	Total	1H	13C
All	55.8 % (135 of 242)	68.6 % (94 of 137)	39.0 % (41 of 105)
Suger, PO4	40.3 % (71 of 176)	57.3 % (55 of 96)	20.0 % (16 of 80)
Nucleobase	97.0 % (64 of 66)	95.1 % (39 of 41)	100.0 % (25 of 25)
Aromatic	96.6 % (56 of 58)	93.9 % (31 of 33)	100.0 % (25 of 25)

1. entity 1

GGAGGAAGGA GCCUCC

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 null, Temperature 311 K, pH 5.6, Details 1 mM RNA, 100% D2O

#	Name	Isotope labeling	Type	Concentration
1	RNA	natural abundance		1 (±0.2) mM
2	KCL	natural abundance		50 mM

Sample #2

Solvent system 100% D2O, Pressure 1 null, Temperature 311 K, pH 5.6, Details 1 mM [U-13C] RNA, 100% D2O

#	Name	Isotope labeling	Type	Concentration
3	RNA	[U-13C]		1 (±0.2) mM
4	KCL	natural abundance		50 mM

Sample #3

Solvent system 10%D2O 90%H2O, Pressure 1 null, Temperature 311 K, pH 5.6, Details 1 mM RNA, 10%D2O 90%H2O

#	Name	Isotope labeling	Type	Concentration
5	RNA	natural abundance		1 (±0.2) mM
6	KCL	natural abundance		50 mM

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Release date

2020-12-16

Citation

Stop codon readthrough in VEGF-A is regulated by complex signals
Wagner, N.O., Edwards, J.E., Abramovich, J., Gupta, P., Swaminathan, H., Rouskin, S., D'Souza, V.M.

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail