BMRBj - BMREntryMaster

Found 1 Document (0.221 seconds)

BMRB: 30818

Ax2 Domain of VEGF Readthrough Element

Authors

D'Souza, V.M., Wagner, N.O., Edwards, J.M.

Assembly

VEGF Ax2

Entity

1. VEGF Ax2 (polymer, Thiol state: not present), 13 monomers, 4236.498 Da Detail

GGUUUCGGGA ACC

Formula weight

4236.498 Da

Source organism

Bos taurus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 52.2 %, Completeness (bb): 33.3 % Detail

Polymer type: polyribonucleotide

	Total	¹H
All	52.2 % (59 of 113)	52.2 % (59 of 113)
Suger, PO4	33.3 % (26 of 78)	33.3 % (26 of 78)
Nucleobase	94.3 % (33 of 35)	94.3 % (33 of 35)
Aromatic	93.1 % (27 of 29)	93.1 % (27 of 29)

1. entity 1

GGUUUCGGGA ACC

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 null, Temperature 311 K, pH 5.6, Details 100 uM RNA, 100% D2O

#	Name	Isotope labeling	Type	Concentration
1	RNA	natural abundance		100 (±10.0) uM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 null, Temperature 311 K, pH 5.6, Details 100 uM RNA, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
2	RNA	natural abundance		100 (±10.0) uM

Release date

2020-12-16

Citation

Stop codon readthrough in VEGF-A is regulated by complex signals
Wagner, N.O., Edwards, J.E., Abramovich, J., Gupta, P., Swaminathan, H., Rouskin, S., D'Souza, V.M.

Experiments performed 2 experiments Detail

Chemical shift validation 3 contents Detail

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	76		100.0 (chain: 1, length: 13)
1	Chemical shift references	chem_shift_reference_1	OK	1		No information

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
1	G	HO2'	5.02879
2	G	HO2'	4.55707
3	U	HO2'	4.55005
4	U	HO2'	4.41324
5	U	HO2'	4.18886
6	C	HO2'	4.39821
7	G	HO2'	4.54922
8	G	HO2'	4.79931
9	G	HO2'	4.84527
10	A	H61	7.83946
10	A	H62	7.02587
10	A	HO2'	4.84561
11	A	H61	8.21234
11	A	H62	6.72362
11	A	HO2'	4.35077
12	C	HO2'	4.20484
13	C	HO2'	4.08094

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	113	59	52.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	78	26	33.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	35	33	94.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	9	9	100.0

Keywords RNA, Stop codon readthrough, VEGF mRNA, stem-loop

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30818_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB