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BMRB: 30832

7LFB

ApoL1 N-terminal domain

Authors

Holliday, M.J., Ultsch, M., Moran, P., Fairbrother, W.J., Kirchhofer, D.

Assembly

Apolipoprotein L1

Entity

1. Apolipoprotein L1 (polymer, Thiol state: not present), 114 monomers, 13287.90 Da Detail

GSDPESSIFI EDAIKYFKEK VSTQNLLLLL TDNEAWNGFV AAAELPRNEA DELRKALDNL ARQMIMKDKN WHDKGQQYRN WFLKEFPRLK SELEDNIRRL RALADGVQKV HKGT

Formula weight

13287.9 Da

Source organism

Exptl. method

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 95.6 %, Completeness: 85.7 %, Completeness (bb): 90.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.7 % (1200 of 1400)	83.9 % (616 of 734)	88.1 % (468 of 531)	85.9 % (116 of 135)
Backbone	90.9 % (616 of 678)	89.1 % (205 of 230)	92.0 % (310 of 337)	91.0 % (101 of 111)
Sidechain	82.3 % (684 of 831)	81.5 % (411 of 504)	85.1 % (258 of 303)	62.5 % (15 of 24)
Aromatic	85.5 % (94 of 110)	85.5 % (47 of 55)	84.6 % (44 of 52)	100.0 % (3 of 3)
Methyl	89.8 % (106 of 118)	89.8 % (53 of 59)	89.8 % (53 of 59)

1. entity 1

GSDPESSIFI EDAIKYFKEK VSTQNLLLLL TDNEAWNGFV AAAELPRNEA DELRKALDNL ARQMIMKDKN WHDKGQQYRN WFLKEFPRLK SELEDNIRRL RALADGVQKV HKGT

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 310 K, pH 5.5, Details 0.9 mM [U-99% 13C; U-99% 15N] ApoL1 (61-172), 93% H2O/7% D2O

#	Name	Isotope labeling	Type	Concentration
1	ApoL1 (61-172)	[U-99% 13C; U-99% 15N]		0.9 mM

Release date

2021-01-28

Citation

Structures of the ApoL1 and ApoL2 N-terminal domains reveal a non-classical four-helix bundle motif
Ultsch, M., Holliday, M.J., Gerhardy, S., Moran, P., Scales, S.J., Gupta, N., Oltrabella, F., Chiu, C., Fairbrother, W., Eigenbrot, C., Kirchhofer, D.
Commun. Biol. (2021), 4, 916-916, PubMed 34316015 , DOI 10.1038/s42003-021-02387-5 , Abstract

Related entities 1. Apolipoprotein L1, : 1 : 3 : 2 : 22 entities Detail

Interaction partners 1. Apolipoprotein L1, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 310 K, pH 5.5, Details 0.9 mM [U-99% 13C; U-99% 15N] ApoL1 (61-172), 93% H2O/7% D2O

#	Name	Isotope labeling	Type	Concentration
1	ApoL1 (61-172)	[U-99% 13C; U-99% 15N]		0.9 mM

2 3D HNCACB

Instrument

Bruker AVANCE - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 310 K, pH 5.5, Details 0.9 mM [U-99% 13C; U-99% 15N] ApoL1 (61-172), 93% H2O/7% D2O

#	Name	Isotope labeling	Type	Concentration
1	ApoL1 (61-172)	[U-99% 13C; U-99% 15N]		0.9 mM

3 3D CBCA(CO)NH

Instrument

Bruker AVANCE - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 310 K, pH 5.5, Details 0.9 mM [U-99% 13C; U-99% 15N] ApoL1 (61-172), 93% H2O/7% D2O

#	Name	Isotope labeling	Type	Concentration
1	ApoL1 (61-172)	[U-99% 13C; U-99% 15N]		0.9 mM

4 3D HNCO

Instrument

Bruker AVANCE - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 310 K, pH 5.5, Details 0.9 mM [U-99% 13C; U-99% 15N] ApoL1 (61-172), 93% H2O/7% D2O

#	Name	Isotope labeling	Type	Concentration
1	ApoL1 (61-172)	[U-99% 13C; U-99% 15N]		0.9 mM

5 3D HN(CA)CO

Instrument

Bruker AVANCE - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 310 K, pH 5.5, Details 0.9 mM [U-99% 13C; U-99% 15N] ApoL1 (61-172), 93% H2O/7% D2O

#	Name	Isotope labeling	Type	Concentration
1	ApoL1 (61-172)	[U-99% 13C; U-99% 15N]		0.9 mM

6 3D HCCH-TOCSY

Instrument

Bruker AVANCE - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 310 K, pH 5.5, Details 0.9 mM [U-99% 13C; U-99% 15N] ApoL1 (61-172), 93% H2O/7% D2O

#	Name	Isotope labeling	Type	Concentration
1	ApoL1 (61-172)	[U-99% 13C; U-99% 15N]		0.9 mM

7 3D 1H-15N NOESY

Instrument

Bruker AVANCE - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 310 K, pH 5.5, Details 0.9 mM [U-99% 13C; U-99% 15N] ApoL1 (61-172), 93% H2O/7% D2O

#	Name	Isotope labeling	Type	Concentration
1	ApoL1 (61-172)	[U-99% 13C; U-99% 15N]		0.9 mM

8 3D (H)CC(CO)NH

Instrument

Bruker AVANCE - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 310 K, pH 5.5, Details 0.9 mM [U-99% 13C; U-99% 15N] ApoL1 (61-172), 93% H2O/7% D2O

#	Name	Isotope labeling	Type	Concentration
1	ApoL1 (61-172)	[U-99% 13C; U-99% 15N]		0.9 mM

9 2D 1H-13C TROSY aromatic

Instrument

Bruker AVANCE - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 310 K, pH 5.5, Details 0.9 mM [U-99% 13C; U-99% 15N] ApoL1 (61-172), 93% H2O/7% D2O

#	Name	Isotope labeling	Type	Concentration
1	ApoL1 (61-172)	[U-99% 13C; U-99% 15N]		0.9 mM

10 2D 1H-13C HSQC

Instrument

Bruker AVANCE - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 310 K, pH 5.5, Details 0.9 mM [U-99% 13C; U-99% 15N] ApoL1 (61-172), 93% H2O/7% D2O

#	Name	Isotope labeling	Type	Concentration
1	ApoL1 (61-172)	[U-99% 13C; U-99% 15N]		0.9 mM

11 3D HNCO

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 310 K, pH 5.5, Details 0.9 mM [U-99% 13C; U-99% 15N] ApoL1 (61-172), 93% H2O/7% D2O

#	Name	Isotope labeling	Type	Concentration
1	ApoL1 (61-172)	[U-99% 13C; U-99% 15N]		0.9 mM

12 3D HN(CA)CO

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 310 K, pH 5.5, Details 0.9 mM [U-99% 13C; U-99% 15N] ApoL1 (61-172), 93% H2O/7% D2O

#	Name	Isotope labeling	Type	Concentration
1	ApoL1 (61-172)	[U-99% 13C; U-99% 15N]		0.9 mM

13 3D 1H-13C NOESY aliphatic

Instrument

Bruker AVANCE - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 310 K, pH 5.5, Details 0.9 mM [U-99% 13C; U-99% 15N] ApoL1 (61-172), 93% H2O/7% D2O

#	Name	Isotope labeling	Type	Concentration
1	ApoL1 (61-172)	[U-99% 13C; U-99% 15N]		0.9 mM

14 3D 1H-13C NOESY aromatic

Instrument

Bruker AVANCE - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 310 K, pH 5.5, Details 0.9 mM [U-99% 13C; U-99% 15N] ApoL1 (61-172), 93% H2O/7% D2O

#	Name	Isotope labeling	Type	Concentration
1	ApoL1 (61-172)	[U-99% 13C; U-99% 15N]		0.9 mM

15 3D HNCA

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 310 K, pH 5.5, Details 0.9 mM [U-99% 13C; U-99% 15N] ApoL1 (61-172), 93% H2O/7% D2O

#	Name	Isotope labeling	Type	Concentration
1	ApoL1 (61-172)	[U-99% 13C; U-99% 15N]		0.9 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30832_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr30832_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1306		95.6 (chain: 1, length: 114)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 114, Sequence coverage: 95.6%
- Total number of assignments
  - ¹H chemical shifts: 726
  - ¹³C chemical shifts: 466
  - ¹⁵N chemical shifts: 114
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords Ion channel, MEMBRANE PROTEIN, kidney disease, lipoprotein