BMRBj - BMREntryMaster

Found 1 Document (0.351 seconds)

BMRB: 30843

7LCW

Aspartimidylated Lasso Peptide Lihuanodin

Authors

Link, A.J., Cao, L.

Assembly

Lasso Peptide Lihuanodin

Entity

1. Lasso Peptide Lihuanodin (polymer, Thiol state: not present), 15 monomers, 1751.763 Da Detail

GSKYSDTADE SSYRW

Formula weight

1751.763 Da

Source organism

Lihuaxuella thermophila

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.5 %, Completeness (bb): 96.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	96.5 % (82 of 85)	96.5 % (82 of 85)
Backbone	96.8 % (30 of 31)	96.8 % (30 of 31)
Sidechain	96.3 % (52 of 54)	96.3 % (52 of 54)
Aromatic	92.9 % (13 of 14)	92.9 % (13 of 14)
Methyl	50.0 % (1 of 2)	50.0 % (1 of 2)

1. entity 1

GSKYSDTADE SSYRW

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 1.6 mM lihuanodin, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	lihuanodin	natural abundance		1.6 mM

Release date

2021-02-16

Citation

Cellulonodin-2 and Lihuanodin: Lasso Peptides with an Aspartimide Post-Translational Modification
Cao, L., Beiser, M., Koos, J.D., Orlova, M., Elashal, H.E., Schroder, H.V., Link, A.J.
J. Am. Chem. Soc. (2021), 143, 11690-11702, PubMed 34283601 , DOI 10.1021/jacs.1c05017 , Abstract

Related entities 1. Lasso Peptide Lihuanodin, : 1 entities Detail

Experiments performed 2 experiments Detail

Chemical shift validation 3 contents Detail

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	84		100.0 (chain: 1, length: 15)
1	Chemical shift references	chem_shift_reference_1	OK	1		No information

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	85	82	96.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	31	30	96.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	54	52	96.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	2	1	50.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	14	13	92.9

Keywords UNKNOWN FUNCTION, post-translational modification

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30843_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

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Found 1 Document (0.351 seconds)

BMRB: 30843

Sample

Related entities 1. Lasso Peptide Lihuanodin, : 1 entities Detail

Experiments performed 2 experiments Detail

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail