BMRBj - BMREntryMaster

Found 1 Document (0.44 seconds)

BMRB: 30858

7LP5

Structure of Nedd4L WW3 domain

Authors

Alam, S.L., Alian, A., Thompson, T., Rheinemann, L., Sundquist, W.I.

Assembly

Angiomotin,E3 ubiquitin-protein ligase NEDD4-like fusion (E.C.2.3.2.26)

Entity

1. Angiomotin,E3 ubiquitin-protein ligase NEDD4-like fusion (E.C.2.3.2.26) (polymer, Thiol state: not present), 68 monomers, 7745.653 Da Detail

MQNNEELPTY EEAKVGGGGG SKVTQSFLPP GWEMRIAPNG RPFFIDHNTK TTTWEDPRLK FPVHMRSK

Formula weight

7745.653 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.1 %, Completeness: 90.6 %, Completeness (bb): 90.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.6 % (738 of 815)	92.3 % (396 of 429)	88.5 % (277 of 313)	89.0 % (65 of 73)
Backbone	90.4 % (356 of 394)	89.0 % (121 of 136)	91.9 % (181 of 197)	88.5 % (54 of 61)
Sidechain	89.6 % (432 of 482)	91.8 % (269 of 293)	85.9 % (152 of 177)	91.7 % (11 of 12)
Aromatic	77.5 % (62 of 80)	92.5 % (37 of 40)	60.5 % (23 of 38)	100.0 % (2 of 2)
Methyl	91.7 % (44 of 48)	91.7 % (22 of 24)	91.7 % (22 of 24)

1. entity 1

MQNNEELPTY EEAKVGGGGG SKVTQSFLPP GWEMRIAPNG RPFFIDHNTK TTTWEDPRLK FPVHMRSK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details [U-100% 13C; U-100% 15N] , 20 mM sodium phosphate, 50 mM sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	Angiomotin,E3 ubiquitin-protein ligase NEDD4-like fusion	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details [U-100% 15N] Angiomotin,E3 ubiquitin-protein ligase NEDD4-like fusion, 20 mM sodium phosphate, 50 mM sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
4	Angiomotin,E3 ubiquitin-protein ligase NEDD4-like fusion	[U-100% 15N]	0.0 ~ 0.0 mM
5	sodium phosphate	natural abundance	20 mM
6	sodium chloride	natural abundance	50 mM

Release date

2021-02-13

Citation

Interactions between AMOT PPxY motifs and NEDD4L WW domains function in HIV-1 release
Rheinemann, L., Thompson, T., Mercenne, G., Paine, E.L., Peterson, F.C., Volkman, B.F., Alam, S.L., Alian, A., Sundquist, W.I.
J. Biol. Chem. (2021), 297, 100975-100975, PubMed 34284061 , DOI 10.1016/j.jbc.2021.100975 , Abstract

Related entities 1. Angiomotin,E3 ubiquitin-protein ligase NEDD4-like fusion (E.C.2.3.2.26), : 1 : 9 entities Detail

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details [U-100% 15N] Angiomotin,E3 ubiquitin-protein ligase NEDD4-like fusion, 20 mM sodium phosphate, 50 mM sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
4	Angiomotin,E3 ubiquitin-protein ligase NEDD4-like fusion	[U-100% 15N]	0.0 ~ 0.0 mM
5	sodium phosphate	natural abundance	20 mM
6	sodium chloride	natural abundance	50 mM

2 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details [U-100% 13C; U-100% 15N] , 20 mM sodium phosphate, 50 mM sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	Angiomotin,E3 ubiquitin-protein ligase NEDD4-like fusion	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

3 3D HNCO

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Angiomotin,E3 ubiquitin-protein ligase NEDD4-like fusion	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

4 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Angiomotin,E3 ubiquitin-protein ligase NEDD4-like fusion	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

5 3D C(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Angiomotin,E3 ubiquitin-protein ligase NEDD4-like fusion	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

6 3D H(CCO)NH

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Angiomotin,E3 ubiquitin-protein ligase NEDD4-like fusion	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

7 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Angiomotin,E3 ubiquitin-protein ligase NEDD4-like fusion	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

8 3D HNHB

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Angiomotin,E3 ubiquitin-protein ligase NEDD4-like fusion	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

9 3D HNHA

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Angiomotin,E3 ubiquitin-protein ligase NEDD4-like fusion	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

10 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Angiomotin,E3 ubiquitin-protein ligase NEDD4-like fusion	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

11 2D 1H-13C HSQC aliphatic

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Angiomotin,E3 ubiquitin-protein ligase NEDD4-like fusion	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

12 2D 1H-13C HSQC aromatic

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Angiomotin,E3 ubiquitin-protein ligase NEDD4-like fusion	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

13 3D 1H-15N NOESY

Instrument

Varian INOVA - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Angiomotin,E3 ubiquitin-protein ligase NEDD4-like fusion	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

14 3D 1H-13C NOESY aliphatic

Instrument

Varian INOVA - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Angiomotin,E3 ubiquitin-protein ligase NEDD4-like fusion	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

15 3D 1H-13C NOESY aromatic

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Angiomotin,E3 ubiquitin-protein ligase NEDD4-like fusion	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30858_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr30858_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	786		97.1 (chain: 1, length: 68)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 68, Sequence coverage: 97.1%
- Total number of assignments
  - ¹H chemical shifts: 444
  - ¹³C chemical shifts: 276
  - ¹⁵N chemical shifts: 66
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	429	396	92.3
¹³C chemical shifts	313	276	88.2
¹⁵N chemical shifts	73	66	90.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	136	123	90.4
¹³C chemical shifts	136	121	89.0
¹⁵N chemical shifts	61	55	90.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	293	273	93.2
¹³C chemical shifts	177	155	87.6
¹⁵N chemical shifts	12	11	91.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	27	24	88.9
¹³C chemical shifts	27	24	88.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	40	37	92.5
¹³C chemical shifts	38	23	60.5
¹⁵N chemical shifts	2	2	100.0

Keywords E3 Ubiquitin ligase, LIGASE, Nedd4L, PPxY binding, WW domain

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Found 1 Document (0.44 seconds)

BMRB: 30858

Sample #1

Sample #2

Related entities 1. Angiomotin,E3 ubiquitin-protein ligase NEDD4-like fusion (E.C.2.3.2.26), : 1 : 9 entities Detail

Experiments performed 15 experiments Detail

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Chemical shift validation 3 contents Detail