BMRBj - BMREntryMaster

Found 1 Document (2.722 seconds)

BMRB: 30861

7LQT

Solution NMR structure of the PNUTS amino-terminal Domain fused to Myc Homology Box 0

Authors

Lemak, A., Wei, Y., Duan, S., Houliston, S., Penn, L.Z., Arrowsmith, C.H.

Assembly

E3 ubiquitin-protein ligase UHRF1 (E.C.2.3.2.27)

Entity

1. E3 ubiquitin-protein ligase UHRF1 (E.C.2.3.2.27) (polymer), 174 monomers, 19368.99 Da Detail

MGSGPIDPKE LLKGLDSFLT RDGEVKSVDG IAKIFSLMKE ARKMVSRCTY LNIILQTRAP EVLVKFIDVG GYKLLNSWLT YSKTTNNIPL LQQILLTLQH LPLTVDHLKQ NNTAKLVKQL SKSSEDEELR KLASVLVSDW MAVIRSQSGG GSGGGSDLDY DSVQPYFYCD EEEN

Formula weight

19368.99 Da

Source organism

Rattus norvegicus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 99.4 %, Completeness: 91.0 %, Completeness (bb): 95.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.0 % (1862 of 2046)	92.0 % (976 of 1061)	89.4 % (710 of 794)	92.1 % (176 of 191)
Backbone	95.8 % (989 of 1032)	96.1 % (341 of 355)	95.9 % (488 of 509)	95.2 % (160 of 168)
Sidechain	86.6 % (1017 of 1175)	88.8 % (627 of 706)	83.9 % (374 of 446)	69.6 % (16 of 23)
Aromatic	71.7 % (86 of 120)	91.7 % (55 of 60)	50.0 % (29 of 58)	100.0 % (2 of 2)
Methyl	97.2 % (208 of 214)	97.2 % (104 of 107)	97.2 % (104 of 107)

1. entity 1

MGSGPIDPKE LLKGLDSFLT RDGEVKSVDG IAKIFSLMKE ARKMVSRCTY LNIILQTRAP EVLVKFIDVG GYKLLNSWLT YSKTTNNIPL LQQILLTLQH LPLTVDHLKQ NNTAKLVKQL SKSSEDEELR KLASVLVSDW MAVIRSQSGG GSGGGSDLDY DSVQPYFYCD EEEN

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.9, Details 20 mM HEPES, 200 mM sodium chloride, 2 mM DTT, 5 % glycerol, 400 uM [U-100% 13C; U-100% 15N] labeled protein, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	HEPES	natural abundance	20 mM
2	sodium chloride	natural abundance	200 mM
3	DTT	natural abundance	2 mM
4	glycerol	natural abundance	5 %
5	labeled protein	[U-100% 13C; U-100% 15N]	400 uM

Release date

2021-02-21

Citation

The MYC oncoprotein directly interacts with its chromatin cofactor PNUTS to recruit PP1 phosphatase
Wei, Y., Redel, C., Ahlner, A., Lemak, A., Johansson-Akhe, I., Houliston, S., Kenney, T., Tamachi, A., Morad, V., Duan, S., Andrews, D.W., Wallner, B., Sunnerhagen, M., Arrowsmith, C.H., Penn, L.Z.
Nucleic Acids Res. (2022), 50, 3505-3522, PubMed 35244724 , DOI 10.1093/nar/gkac138 , Abstract

Related entities 1. E3 ubiquitin-protein ligase UHRF1 (E.C.2.3.2.27), : 1 : 3 : 16 entities Detail

Interaction partners 1. E3 ubiquitin-protein ligase UHRF1 (E.C.2.3.2.27), : 8 interactors Detail

More details for 8 interactors identified by 3 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.9, Details 20 mM HEPES, 200 mM sodium chloride, 2 mM DTT, 5 % glycerol, 400 uM [U-100% 13C; U-100% 15N] labeled protein, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	HEPES	natural abundance	20 mM
2	sodium chloride	natural abundance	200 mM
3	DTT	natural abundance	2 mM
4	glycerol	natural abundance	5 %
5	labeled protein	[U-100% 13C; U-100% 15N]	400 uM

2 3D HN(CO)CA

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	HEPES	natural abundance	20 mM
2	sodium chloride	natural abundance	200 mM
3	DTT	natural abundance	2 mM
4	glycerol	natural abundance	5 %
5	labeled protein	[U-100% 13C; U-100% 15N]	400 uM

3 3D HNCA

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	HEPES	natural abundance	20 mM
2	sodium chloride	natural abundance	200 mM
3	DTT	natural abundance	2 mM
4	glycerol	natural abundance	5 %
5	labeled protein	[U-100% 13C; U-100% 15N]	400 uM

4 3D CBCA(CO)NH

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	HEPES	natural abundance	20 mM
2	sodium chloride	natural abundance	200 mM
3	DTT	natural abundance	2 mM
4	glycerol	natural abundance	5 %
5	labeled protein	[U-100% 13C; U-100% 15N]	400 uM

5 3D HNCO

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	HEPES	natural abundance	20 mM
2	sodium chloride	natural abundance	200 mM
3	DTT	natural abundance	2 mM
4	glycerol	natural abundance	5 %
5	labeled protein	[U-100% 13C; U-100% 15N]	400 uM

6 2D 1H-13C HSQC aromatic

Instrument

Bruker AVANCE II - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	HEPES	natural abundance	20 mM
2	sodium chloride	natural abundance	200 mM
3	DTT	natural abundance	2 mM
4	glycerol	natural abundance	5 %
5	labeled protein	[U-100% 13C; U-100% 15N]	400 uM

7 2D 1H-13C HSQC aliphatic

Instrument

Bruker AVANCE II - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	HEPES	natural abundance	20 mM
2	sodium chloride	natural abundance	200 mM
3	DTT	natural abundance	2 mM
4	glycerol	natural abundance	5 %
5	labeled protein	[U-100% 13C; U-100% 15N]	400 uM

8 3D 1H-15N NOESY

Instrument

Bruker AVANCE II - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	HEPES	natural abundance	20 mM
2	sodium chloride	natural abundance	200 mM
3	DTT	natural abundance	2 mM
4	glycerol	natural abundance	5 %
5	labeled protein	[U-100% 13C; U-100% 15N]	400 uM

9 3D HNCACO

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	HEPES	natural abundance	20 mM
2	sodium chloride	natural abundance	200 mM
3	DTT	natural abundance	2 mM
4	glycerol	natural abundance	5 %
5	labeled protein	[U-100% 13C; U-100% 15N]	400 uM

10 3D HBHA(CO)NH

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	HEPES	natural abundance	20 mM
2	sodium chloride	natural abundance	200 mM
3	DTT	natural abundance	2 mM
4	glycerol	natural abundance	5 %
5	labeled protein	[U-100% 13C; U-100% 15N]	400 uM

11 3D 1H-13C NOESY aromatic

Instrument

Bruker AVANCE II - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	HEPES	natural abundance	20 mM
2	sodium chloride	natural abundance	200 mM
3	DTT	natural abundance	2 mM
4	glycerol	natural abundance	5 %
5	labeled protein	[U-100% 13C; U-100% 15N]	400 uM

12 3D 1H-13C NOESY aliphatic

Instrument

Bruker AVANCE II - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	HEPES	natural abundance	20 mM
2	sodium chloride	natural abundance	200 mM
3	DTT	natural abundance	2 mM
4	glycerol	natural abundance	5 %
5	labeled protein	[U-100% 13C; U-100% 15N]	400 uM

13 3D HCCH-TOCSY

Instrument

Bruker AVANCE II - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	HEPES	natural abundance	20 mM
2	sodium chloride	natural abundance	200 mM
3	DTT	natural abundance	2 mM
4	glycerol	natural abundance	5 %
5	labeled protein	[U-100% 13C; U-100% 15N]	400 uM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30861_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr30861_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	2062		99.4 (chain: 1, length: 174)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 174, Sequence coverage: 99.4%
- Total number of assignments
  - ¹H chemical shifts: 1184
  - ¹³C chemical shifts: 702
  - ¹⁵N chemical shifts: 176
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	1061	970	91.4
¹³C chemical shifts	794	702	88.4
¹⁵N chemical shifts	191	176	92.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	355	340	95.8
¹³C chemical shifts	348	328	94.3
¹⁵N chemical shifts	168	160	95.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	706	630	89.2
¹³C chemical shifts	446	374	83.9
¹⁵N chemical shifts	23	16	69.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	111	107	96.4
¹³C chemical shifts	111	107	96.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	60	55	91.7
¹³C chemical shifts	58	29	50.0
¹⁵N chemical shifts	2	2	100.0

Keywords Myc Box 0, NUCLEAR PROTEIN, PP1-PNUTS phosphatase complex, Regulatory Subunit, c-MYC oncoprotein

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Found 1 Document (2.722 seconds)

BMRB: 30861

Sample

Related entities 1. E3 ubiquitin-protein ligase UHRF1 (E.C.2.3.2.27), : 1 : 3 : 16 entities Detail

Interaction partners 1. E3 ubiquitin-protein ligase UHRF1 (E.C.2.3.2.27), : 8 interactors Detail

Experiments performed 13 experiments Detail

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Chemical shift validation 3 contents Detail