BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	32.4 % (289 of 893)	16.8 % (77 of 457)	40.9 % (146 of 357)	83.5 % (66 of 79)
Backbone	60.9 % (273 of 448)	44.4 % (67 of 151)	62.7 % (141 of 225)	90.3 % (65 of 72)
Sidechain	16.4 % (85 of 518)	3.3 % (10 of 306)	36.1 % (74 of 205)	14.3 % (1 of 7)
Aromatic	5.3 % (2 of 38)	5.3 % (1 of 19)	0.0 % (0 of 18)	100.0 % (1 of 1)
Methyl	8.1 % (10 of 124)	6.5 % (4 of 62)	9.7 % (6 of 62)

1. entity 1

AGHMTDTLFL TVTASLTLPW DHIQSTLLKT KGVTDIKIYP QKRTVAVTII PSIVNANQIK ELVPELSLDT GTLEKK

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.4 (±0.02), Details 0.35 mM [U-13C; U-15N] HMA2A, 50 mM HEPES, 150 mM sodium chloride, 5 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	HMA2A	[U-13C; U-15N]	0.35 (±0.02) mM
2	HEPES	natural abundance	50 (±1.0) mM
3	sodium chloride	natural abundance	150 (±1.0) mM
4	TCEP	natural abundance	5 (±0.2) mM

Release date

2021-03-21

Citation

At sixes and sevens: cryptic domain in the metal binding chain of the human copper transporter ATP7A
Uhlemann, E.E., Lee, W., Tonelli, M., Dmitriev, O.Y.
Biophys. J. (2021), 120, 4600-4607, PubMed 34461106 , DOI 10.1016/j.bpj.2021.08.029 , Abstract

Related entities 1. Copper-transporting ATPase 1 (E.C.7.2.2.8), : 9 entities Detail

Interaction partners 1. Copper-transporting ATPase 1 (E.C.7.2.2.8), : 5 interactors Detail

More details for 5 interactors identified by 3 citations

Experiments performed 5 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.4 (±0.02), Details 0.35 mM [U-13C; U-15N] HMA2A, 50 mM HEPES, 150 mM sodium chloride, 5 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	HMA2A	[U-13C; U-15N]	0.35 (±0.02) mM
2	HEPES	natural abundance	50 (±1.0) mM
3	sodium chloride	natural abundance	150 (±1.0) mM
4	TCEP	natural abundance	5 (±0.2) mM

2 3D HNCA

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	HMA2A	[U-13C; U-15N]	0.35 (±0.02) mM
2	HEPES	natural abundance	50 (±1.0) mM
3	sodium chloride	natural abundance	150 (±1.0) mM
4	TCEP	natural abundance	5 (±0.2) mM

3 3D HNCACB

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	HMA2A	[U-13C; U-15N]	0.35 (±0.02) mM
2	HEPES	natural abundance	50 (±1.0) mM
3	sodium chloride	natural abundance	150 (±1.0) mM
4	TCEP	natural abundance	5 (±0.2) mM

4 3D 1H-15N NOESY

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	HMA2A	[U-13C; U-15N]	0.35 (±0.02) mM
2	HEPES	natural abundance	50 (±1.0) mM
3	sodium chloride	natural abundance	150 (±1.0) mM
4	TCEP	natural abundance	5 (±0.2) mM

5 3D CBCA(CO)NH

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	HMA2A	[U-13C; U-15N]	0.35 (±0.02) mM
2	HEPES	natural abundance	50 (±1.0) mM
3	sodium chloride	natural abundance	150 (±1.0) mM
4	TCEP	natural abundance	5 (±0.2) mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr30866_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr30866_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	273		94.7 (chain: 1, length: 76)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 76, Sequence coverage: 94.7%
- Total number of assignments
  - ¹³C chemical shifts: 141
  - ¹H chemical shifts: 66
  - ¹⁵N chemical shifts: 66
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	357	141	39.5
¹H chemical shifts	457	66	14.4
¹⁵N chemical shifts	79	66	83.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	152	72	47.4
¹H chemical shifts	151	65	43.0
¹⁵N chemical shifts	72	65	90.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	205	69	33.7
¹H chemical shifts	306	1	0.3
¹⁵N chemical shifts	7	1	14.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	63	2	3.2
¹H chemical shifts	63	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	18	0	0.0
¹H chemical shifts	19	1	5.3
¹⁵N chemical shifts	1	1	100.0

Keywords ATP7A, METAL TRANSPORT, copper transport, heavy metal associated domain, membrane transporter

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Found 1 Document (0.857 seconds)

BMRB: 30866

Sample

Related entities 1. Copper-transporting ATPase 1 (E.C.7.2.2.8), : 9 entities Detail

Interaction partners 1. Copper-transporting ATPase 1 (E.C.7.2.2.8), : 5 interactors Detail

Experiments performed 5 experiments Detail

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Chemical shift validation 3 contents Detail