BMRB: 30868

Solution structure of the HIV-1 PBS-segment

Authors

Heng, X., Song, Z.

Assembly

RNA (103-MER)

Entity

1. RNA (103-MER) (polymer, Thiol state: not present), 103 monomers, 33312.80 Da Detail

GGCUCUGGUA ACUAGAGAUC CCUCAGACCC UUUUAGUCAG UGUGGAAAAU CUCUAGCAGU GGCGCCCGAA CAGGGACUUG AAAGCGAAAG UAAAGCCAGA GCC

Formula weight

33312.8 Da

Source organism

Human immunodeficiency virus 1

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 53.0 %, Completeness (bb): 48.4 % Detail

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Polymer type: polyribonucleotide

	Total	1H
All	53.0 % (474 of 894)	53.0 % (474 of 894)
Suger, PO4	48.4 % (299 of 618)	48.4 % (299 of 618)
Nucleobase	63.4 % (175 of 276)	63.4 % (175 of 276)
Aromatic	77.4 % (175 of 226)	77.4 % (175 of 226)

1. entity 1

GGCUCUGGUA ACUAGAGAUC CCUCAGACCC UUUUAGUCAG UGUGGAAAAU CUCUAGCAGU GGCGCCCGAA CAGGGACUUG AAAGCGAAAG UAAAGCCAGA GCC

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.5, Details 250 uM 1H RNA (103-MER), 100% D2O

#	Name	Isotope labeling	Type	Concentration
1	RNA (103-MER)	1H		250 uM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.5, Details 250 uM A-8D, C-D, G-H, U-5,6-D2 RNA (103-MER), 100% D2O

#	Name	Isotope labeling	Type	Concentration
2	RNA (103-MER)	[A-8D, C-D, G-H, U-5,6-D2]		250 uM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.5, Details 250 uM A-8D, C-D, G-8D, U-H RNA (103-MER), 100% D2O

#	Name	Isotope labeling	Type	Concentration
3	RNA (103-MER)	[A-8D, C-D, G-8D, U-H]		250 uM

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.5, Details 250 uM A-8D, C-5,6-D2, G-D, U-5,6-D2 RNA (103-MER), 100% D2O

#	Name	Isotope labeling	Type	Concentration
4	RNA (103-MER)	[A-8D, C-5,6-D2, G-D, U-5,6-D2]		250 uM

Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.5, Details 250 uM A-8D, C-D, G-8D, U-D RNA (103-MER), 100% D2O

#	Name	Isotope labeling	Type	Concentration
5	RNA (103-MER)	[A-8D, C-D, G-8D, U-D]		250 uM

Sample #6

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.5, Details 250 uM A-H, C-D, G-H, U-D RNA (103-MER), 100% D2O

#	Name	Isotope labeling	Type	Concentration
6	RNA (103-MER)	[A-H, C-D, G-H, U-D]		250 uM

Sample #7

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.5, Details 250 uM A-H, C-H, G-D, U-D RNA (103-MER), 100% D2O

#	Name	Isotope labeling	Type	Concentration
7	RNA (103-MER)	[A-H, C-H, G-D, U-D]		250 uM

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Release date

2021-03-09

Citation

The three-way junction structure of the HIV-1 PBS-segment binds host enzyme important for viral infectivity
Song, Z., Gremminger, T., Singh, G., Cheng, Y., Li, J., Qiu, L., Ji, J., Lange, M.J., Zuo, X., Chen, S.J., Zou, X., Boris-Lawrie, K., Heng, X.
Nucleic Acids Res. (2021), 49, 5925-5942, PubMed 33978756 , DOI 10.1093/nar/gkab342 , Abstract

Related entities 1. RNA (103-MER), : 1 : 5 entities Detail

Experiments performed 7 experiments Detail

Chemical shift validation 3 contents Detail