BMRBj - BMREntryMaster

Found 1 Document (1.892 seconds)

BMRB: 30877

7M0G

Magic Angle Spinning NMR Structure of Human Cofilin-2 Assembled on Actin Filaments

Authors

Kraus, J., Polenova, T., Perilla, J.P., Xu, C.

Assembly

Cofilin-2

Entity

1. Cofilin-2 (polymer), 166 monomers, 18736.38 Da Detail

MASGVTVNDE VIKVFNDMKV RKSSTQEEIK KRKKAVLFCL SDDKRQIIVE EAKQILVGDI GDTVEDPYTS FVKLLPLNDC RYALYDATYE TKESKKEDLV FIFWAPESAP LKSKMIYASS KDAIKKKFTG IKHEWQVNGL DDIKDRSTLG EKLGGNVVVS LEGKPL

Formula weight

18736.38 Da

Source organism

Homo sapiens

Exptl. method

solid-state NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 94.1 %, Completeness (bb): 97.4 % Detail

Polymer type: polypeptide(L)

	Total	¹³C	¹⁵N
All	94.1 % (897 of 953)	94.8 % (736 of 776)	91.0 % (161 of 177)
Backbone	97.4 % (633 of 650)	97.1 % (475 of 489)	98.1 % (158 of 161)
Sidechain	91.1 % (419 of 460)	93.7 % (416 of 444)	18.8 % (3 of 16)
Aromatic	79.7 % (51 of 64)	82.3 % (51 of 62)	0.0 % (0 of 2)
Methyl	86.6 % (84 of 97)	86.6 % (84 of 97)

1. entity 1

MASGVTVNDE VIKVFNDMKV RKSSTQEEIK KRKKAVLFCL SDDKRQIIVE EAKQILVGDI GDTVEDPYTS FVKLLPLNDC RYALYDATYE TKESKKEDLV FIFWAPESAP LKSKMIYASS KDAIKKKFTG IKHEWQVNGL DDIKDRSTLG EKLGGNVVVS LEGKPL

Show sample condition

Sample #1

Solvent system solid, Pressure 1 atm, Temperature 273 K, pH 6.6, Details 20.8 % w/w [U-13C; U-15N] human cofilin-2, 79.2 % w/w NA-actin F-actin, solid

#	Name	Isotope labeling	Type	Concentration
1	human cofilin-2	[U-13C; U-15N]		20.8 % w/w
2	F-actin	NA-actin		79.2 % w/w

Sample #2

Solvent system solid, Pressure 1 atm, Temperature 273 K, pH 6.6, Details 20.8 % w/w [1,6-13C]-glucose, U-15N human cofilin-2, 79.2 % w/w NA-actin F-actin, solid

#	Name	Isotope labeling	Type	Concentration
3	human cofilin-2	[1,6-13C]-glucose, U-15N		20.8 % w/w
4	F-actin	NA-actin		79.2 % w/w

Sample #3

Solvent system solid, Pressure 1 atm, Temperature 273 K, pH 6.6, Details 20.8 % w/w [2-13C]-glucose, U-15N human cofilin-2, 79.2 % w/w NA-actin F-actin, solid

#	Name	Isotope labeling	Type	Concentration
5	human cofilin-2	[2-13C]-glucose, U-15N		20.8 % w/w
6	F-actin	NA-actin		79.2 % w/w

Release date

2021-04-11

Citation

Magic angle spinning NMR structure of human cofilin-2 assembled on actin filaments reveals isoform-specific conformation and binding mode
Kraus, J., Russell, R.W., Kudryashova, E., Xu, C., Katyal, N., Perilla, J.R., Kudryashov, D.S., Polenova, T.
Nat. Commun. (2022), 13, 2114-2114, PubMed 35440100 , DOI 10.1038/s41467-022-29595-9 , Abstract

Related entities 1. Cofilin-2, : 1 : 3 : 3 : 159 entities Detail

Interaction partners 1. Cofilin-2, : 27 interactors Detail

More details for 27 interactors identified by 8 citations

Experiments performed 13 experiments Detail

1 Exp1_CORD50ms

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system solid, Pressure 1 atm, Temperature 273 K, pH 6.6, Details 20.8 % w/w [U-13C; U-15N] human cofilin-2, 79.2 % w/w NA-actin F-actin, solid

#	Name	Isotope labeling	Type	Concentration
1	human cofilin-2	[U-13C; U-15N]		20.8 % w/w
2	F-actin	NA-actin		79.2 % w/w

2 Exp2_CORD200ms

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system solid, Pressure 1 atm, Temperature 273 K, pH 6.6, Details 20.8 % w/w [U-13C; U-15N] human cofilin-2, 79.2 % w/w NA-actin F-actin, solid

#	Name	Isotope labeling	Type	Concentration
1	human cofilin-2	[U-13C; U-15N]		20.8 % w/w
2	F-actin	NA-actin		79.2 % w/w

3 Exp3_CORD50ms

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system solid, Pressure 1 atm, Temperature 273 K, pH 6.6, Details 20.8 % w/w [1,6-13C]-glucose, U-15N human cofilin-2, 79.2 % w/w NA-actin F-actin, solid

#	Name	Isotope labeling	Type	Concentration
3	human cofilin-2	[1,6-13C]-glucose, U-15N		20.8 % w/w
4	F-actin	NA-actin		79.2 % w/w

4 Exp4_CORD200ms

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system solid, Pressure 1 atm, Temperature 273 K, pH 6.6, Details 20.8 % w/w [1,6-13C]-glucose, U-15N human cofilin-2, 79.2 % w/w NA-actin F-actin, solid

#	Name	Isotope labeling	Type	Concentration
3	human cofilin-2	[1,6-13C]-glucose, U-15N		20.8 % w/w
4	F-actin	NA-actin		79.2 % w/w

5 Exp5_CORD500ms

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system solid, Pressure 1 atm, Temperature 273 K, pH 6.6, Details 20.8 % w/w [1,6-13C]-glucose, U-15N human cofilin-2, 79.2 % w/w NA-actin F-actin, solid

#	Name	Isotope labeling	Type	Concentration
3	human cofilin-2	[1,6-13C]-glucose, U-15N		20.8 % w/w
4	F-actin	NA-actin		79.2 % w/w

6 Exp6_CORD50ms

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system solid, Pressure 1 atm, Temperature 273 K, pH 6.6, Details 20.8 % w/w [2-13C]-glucose, U-15N human cofilin-2, 79.2 % w/w NA-actin F-actin, solid

#	Name	Isotope labeling	Type	Concentration
5	human cofilin-2	[2-13C]-glucose, U-15N		20.8 % w/w
6	F-actin	NA-actin		79.2 % w/w

7 Exp7_CORD200ms

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system solid, Pressure 1 atm, Temperature 273 K, pH 6.6, Details 20.8 % w/w [2-13C]-glucose, U-15N human cofilin-2, 79.2 % w/w NA-actin F-actin, solid

#	Name	Isotope labeling	Type	Concentration
5	human cofilin-2	[2-13C]-glucose, U-15N		20.8 % w/w
6	F-actin	NA-actin		79.2 % w/w

8 Exp8_CORD500ms

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system solid, Pressure 1 atm, Temperature 273 K, pH 6.6, Details 20.8 % w/w [2-13C]-glucose, U-15N human cofilin-2, 79.2 % w/w NA-actin F-actin, solid

#	Name	Isotope labeling	Type	Concentration
5	human cofilin-2	[2-13C]-glucose, U-15N		20.8 % w/w
6	F-actin	NA-actin		79.2 % w/w

9 Exp9_PAINCP

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system solid, Pressure 1 atm, Temperature 273 K, pH 6.6, Details 20.8 % w/w [2-13C]-glucose, U-15N human cofilin-2, 79.2 % w/w NA-actin F-actin, solid

#	Name	Isotope labeling	Type	Concentration
5	human cofilin-2	[2-13C]-glucose, U-15N		20.8 % w/w
6	F-actin	NA-actin		79.2 % w/w

10 Exp10_NCACX2d

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system solid, Pressure 1 atm, Temperature 273 K, pH 6.6, Details 20.8 % w/w [U-13C; U-15N] human cofilin-2, 79.2 % w/w NA-actin F-actin, solid

#	Name	Isotope labeling	Type	Concentration
1	human cofilin-2	[U-13C; U-15N]		20.8 % w/w
2	F-actin	NA-actin		79.2 % w/w

11 Exp11_NCACX3d

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system solid, Pressure 1 atm, Temperature 273 K, pH 6.6, Details 20.8 % w/w [U-13C; U-15N] human cofilin-2, 79.2 % w/w NA-actin F-actin, solid

#	Name	Isotope labeling	Type	Concentration
1	human cofilin-2	[U-13C; U-15N]		20.8 % w/w
2	F-actin	NA-actin		79.2 % w/w

12 Exp12_NCOCX2d

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system solid, Pressure 1 atm, Temperature 273 K, pH 6.6, Details 20.8 % w/w [U-13C; U-15N] human cofilin-2, 79.2 % w/w NA-actin F-actin, solid

#	Name	Isotope labeling	Type	Concentration
1	human cofilin-2	[U-13C; U-15N]		20.8 % w/w
2	F-actin	NA-actin		79.2 % w/w

13 Exp13_NCOCX3d

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system solid, Pressure 1 atm, Temperature 273 K, pH 6.6, Details 20.8 % w/w [U-13C; U-15N] human cofilin-2, 79.2 % w/w NA-actin F-actin, solid

#	Name	Isotope labeling	Type	Concentration
1	human cofilin-2	[U-13C; U-15N]		20.8 % w/w
2	F-actin	NA-actin		79.2 % w/w

nullKeywords CONTRACTILE PROTEIN, actin filament binding, actin filament severing, cytoskeletal assembly

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Found 1 Document (1.892 seconds)

BMRB: 30877

Sample #1

Sample #2

Sample #3

Related entities 1. Cofilin-2, : 1 : 3 : 3 : 159 entities Detail

Interaction partners 1. Cofilin-2, : 27 interactors Detail

Experiments performed 13 experiments Detail

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