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NMR structure of the Human T-cell leukemia virus 1 matrix protein
Authors
Herrmann, D., Saad, J.S.
Assembly
Matrix protein p19
Entity
1. Matrix protein p19 (polymer), 105 monomers, 12005.81 Da Detail

MGRIFSRSAS PIPRPPRGLA AHHWLNFLQA AYRLEPGPSS YDFHQLKKFL KIALETPVWI CPINYSLLAS LLPKGYPGRV NEILHILIQT QAQIPSRPAH HHHHH


Formula weight
12005.81 Da
Source organism
Human T-cell leukemia virus type I
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 92.4 %, Completeness: 86.0 %, Completeness (bb): 88.8 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All86.0 % (1110 of 1290)85.9 % (577 of 672)86.4 % (439 of 508)85.5 % (94 of 110)
Backbone88.8 % (538 of 606)89.2 % (181 of 203)87.7 % (272 of 310)91.4 % (85 of 93)
Sidechain84.7 % (664 of 784)84.4 % (396 of 469)86.9 % (259 of 298)52.9 % (9 of 17)
Aromatic77.9 % (106 of 136)79.4 % (54 of 68)75.8 % (50 of 66)100.0 % (2 of 2)
Methyl97.5 % (115 of 118)98.3 % (58 of 59)96.6 % (57 of 59)

1. entity 1

MGRIFSRSAS PIPRPPRGLA AHHWLNFLQA AYRLEPGPSS YDFHQLKKFL KIALETPVWI CPINYSLLAS LLPKGYPGRV NEILHILIQT QAQIPSRPAH HHHHH

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0, Details 100 mM sodium chloride, 50 mM sodium phosphate, 2 mM TCEP, 400 uM [U-99% 13C; U-99% 15N] HTLV-1 Matrix, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
1sodium chloridenatural abundance100 mM
2sodium phosphatenatural abundance50 mM
3TCEPnatural abundance2 mM
4HTLV-1 Matrix[U-99% 13C; U-99% 15N]400 uM
Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0, Details 100 mM sodium chloride, 50 mM sodium phosphate, 2 mM TCEP, 320 uM [U-99% 13C; U-99% 15N] HTLV-1 Matrix, 100% D2O


#NameIsotope labelingTypeConcentration
5sodium chloridenatural abundance100 mM
6sodium phosphatenatural abundance50 mM
7TCEPnatural abundance2 mM
8HTLV-1 Matrix[U-99% 13C; U-99% 15N]320 uM
Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0, Details 100 mM sodium chloride, 50 mM sodium phosphate, 2 mM TCEP, 500 uM [U-99% 15N] HTLV-1 Matrix, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
9sodium chloridenatural abundance100 mM
10sodium phosphatenatural abundance50 mM
11TCEPnatural abundance2 mM
12HTLV-1 Matrix[U-99% 15N]500 uM

Release date
2021-03-17
Citation
Structural Insights into the Mechanism of Human T-cell Leukemia Virus Type 1 Gag Targeting to the Plasma Membrane for Assembly
Herrmann, D., Zhou, L.W., Hanson, H.M., Willkomm, N.A., Mansky, L.M., Saad, J.S.
J. Mol. Biol. (2021), 433, 167161-167161, PubMed 34298060 , DOI 10.1016/j.jmb.2021.167161 ,
Related entities 1. Matrix protein p19, : 1 : 20 entities Detail
Experiments performed 11 experiments Detail
Chemical shift validation 3 contents Detail
Keywords Gag, HTLV-1, Human T-cell leukemia virus 1, Matrix, VIRAL PROTEIN, membrane-binding protein