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AUGalpha - FAM150B - ALKL2 77-152
Authors
Rossi, P., Sowaileh, M., Reshetnyak, A.V., Kalodimos, C.G.
Assembly
ALK and LTK ligand 2
Entity
1. ALK and LTK ligand 2 (polymer, Thiol state: all disulfide bound), 76 monomers, 8988.455 Da Detail

GPSPEQRVEI VPRDLRMKDK FLKHLTGPLY FSPKCSKHFH RLYHNTRDCT IPAYYKRCAR LLTRLAVSPV CMEDKQ


Formula weight
8988.455 Da
Entity Connection
disulfide 2 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1disulfidesing1:CYS35:SG1:CYS71:SG
2disulfidesing1:CYS49:SG1:CYS58:SG

Source organism
Homo sapiens
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 94.7 %, Completeness: 83.2 %, Completeness (bb): 78.3 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All83.2 % (785 of 944)88.9 % (442 of 497)75.7 % (278 of 367)81.3 % (65 of 80)
Backbone78.3 % (346 of 442)93.2 % (137 of 147)63.7 % (144 of 226)94.2 % (65 of 69)
Sidechain88.0 % (507 of 576)87.1 % (305 of 350)94.0 % (202 of 215) 0.0 % (0 of 11)
Aromatic97.4 % (76 of 78)97.4 % (38 of 39)97.4 % (38 of 39)
Methyl100.0 % (70 of 70)100.0 % (35 of 35)100.0 % (35 of 35)

1. entity 1

GPSPEQRVEI VPRDLRMKDK FLKHLTGPLY FSPKCSKHFH RLYHNTRDCT IPAYYKRCAR LLTRLAVSPV CMEDKQ

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 300 uM [U-100% 13C; U-100% 15N] AUGalpha-FAM150B- residues 77-152, 150 mM sodium chloride, 20 mM sodium phosphate, 0.1 % sodium azide, 90% H2O/10% D2O


#NameIsotope labelingTypeConcentration
1AUGalpha-FAM150B- residues 77-152[U-100% 13C; U-100% 15N]300 uM
2sodium chloridenatural abundance150 mM
3sodium phosphatenatural abundance20 mM
4sodium azidenatural abundance0.1 %
Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 300 uM U-15N, U-1H,13C_CH3_AILMTV AUGalpha-FAM150B- residues 77-152, 150 mM sodium chloride, 20 mM sodium phosphate, 0.1 % sodium azide, 90% H2O/10% D2O


#NameIsotope labelingTypeConcentration
5AUGalpha-FAM150B- residues 77-152[U-15N; U-1H,13C_CH3_AILMTV]300 uM
6sodium chloridenatural abundance150 mM
7sodium phosphatenatural abundance20 mM
8sodium azidenatural abundance0.1 %

Release date
2021-06-17
Citation
Mechanism for the activation of the anaplastic lymphoma kinase receptor
Reshetnyak, A.V., Rossi, P., Myasnikov, A.G., Sowaileh, M., Mohanty, J., Nourse, A., Miller, D.J., Lax, I., Schlessinger, J., Kalodimos, C.G.
Nature (2021), 600, 153-157, PubMed 34819673 , DOI 10.1038/s41586-021-04140-8 ,
Related entities 1. ALK and LTK ligand 2, : 1 : 3 : 2 : 16 entities Detail
Interaction partners 1. ALK and LTK ligand 2, : 1 interactors Detail
Experiments performed 17 experiments Detail
Chemical shift validation 3 contents Detail
Keywords ALKL2 77-152, Anaplastic lymphoma kinase activating ligand, CYTOKINE, FAM150B