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BMRB: 30927

7N99

SDE2-apo

Authors

Paung, Y., Weinheimer, A.S., Rageul, J., Khan, A., Ho, B., Tong, M., Johnson, B., Seeliger, M.A., Kim, H.

Assembly

Isoform 2 of Replication stress response regulator SDE2

Entity

1. Isoform 2 of Replication stress response regulator SDE2 (polymer, Thiol state: all free), 77 monomers, 8281.791 Da Detail

GPLGSIDKET IDLLAFTSVA ELELLGLEKL KCELMALGLK CGGTLQERAA RLFSVRGLAK EQIDPALFAK PLKGKKK

Formula weight

8281.791 Da

Source organism

Exptl. method

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 98.7 %, Completeness: 72.1 %, Completeness (bb): 95.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	72.1 % (655 of 909)	59.2 % (281 of 475)	84.8 % (301 of 355)	92.4 % (73 of 79)
Backbone	95.4 % (435 of 456)	91.2 % (145 of 159)	97.3 % (217 of 223)	98.6 % (73 of 74)
Sidechain	55.4 % (289 of 522)	43.0 % (136 of 316)	76.1 % (153 of 201)	0.0 % (0 of 5)
Aromatic	0.0 % (0 of 30)	0.0 % (0 of 15)	0.0 % (0 of 15)
Methyl	32.1 % (34 of 106)	1.9 % (1 of 53)	62.3 % (33 of 53)

1. entity 1

GPLGSIDKET IDLLAFTSVA ELELLGLEKL KCELMALGLK CGGTLQERAA RLFSVRGLAK EQIDPALFAK PLKGKKK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 300 uM [U-100% 13C; U-100% 15N] SDE2-DNA Binding protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	SDE2-DNA Binding protein	[U-100% 13C; U-100% 15N]		300 uM
2	Na/K phosphate	natural abundance		50 mM
3	NaCl	natural abundance		50 mM
4	DTT	natural abundance		5 mM

Release date

2021-07-18

Citation

Extended DNA-binding interfaces beyond the canonical SAP domain contribute to the function of replication stress regulator SDE2 at DNA replication forks
Weinheimer, A.S., Paung, Y., Rageul, J., Khan, A., Lo, N., Ho, B., Tong, M., Alphonse, S., Seeliger, M.A., Kim, H.
J. Biol. Chem. (2022), 298, 102268-102268, PubMed 35850305 , DOI 10.1016/j.jbc.2022.102268 , Abstract

Related entities 1. Isoform 2 of Replication stress response regulator SDE2, : 1 : 3 : 44 entities Detail

Interaction partners 1. Isoform 2 of Replication stress response regulator SDE2, : 4 interactors Detail

More details for 4 interactors identified by 2 citations

Experiments performed 12 experiments Detail

1 3D HNCA

Instrument

Bruker AVANCE III HD - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 300 uM [U-100% 13C; U-100% 15N] SDE2-DNA Binding protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	SDE2-DNA Binding protein	[U-100% 13C; U-100% 15N]		300 uM
2	Na/K phosphate	natural abundance		50 mM
3	NaCl	natural abundance		50 mM
4	DTT	natural abundance		5 mM

2 3DHNCACO

Instrument

Bruker AVANCE III HD - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 300 uM [U-100% 13C; U-100% 15N] SDE2-DNA Binding protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	SDE2-DNA Binding protein	[U-100% 13C; U-100% 15N]		300 uM
2	Na/K phosphate	natural abundance		50 mM
3	NaCl	natural abundance		50 mM
4	DTT	natural abundance		5 mM

3 3D HN(CO)CA

Instrument

Bruker AVANCE III HD - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 300 uM [U-100% 13C; U-100% 15N] SDE2-DNA Binding protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	SDE2-DNA Binding protein	[U-100% 13C; U-100% 15N]		300 uM
2	Na/K phosphate	natural abundance		50 mM
3	NaCl	natural abundance		50 mM
4	DTT	natural abundance		5 mM

4 3D HNCO

Instrument

Bruker AVANCE III HD - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 300 uM [U-100% 13C; U-100% 15N] SDE2-DNA Binding protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	SDE2-DNA Binding protein	[U-100% 13C; U-100% 15N]		300 uM
2	Na/K phosphate	natural abundance		50 mM
3	NaCl	natural abundance		50 mM
4	DTT	natural abundance		5 mM

5 3D HNCACB

Instrument

Bruker AVANCE III HD - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 300 uM [U-100% 13C; U-100% 15N] SDE2-DNA Binding protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	SDE2-DNA Binding protein	[U-100% 13C; U-100% 15N]		300 uM
2	Na/K phosphate	natural abundance		50 mM
3	NaCl	natural abundance		50 mM
4	DTT	natural abundance		5 mM

6 3D HN(COCA)CB

Instrument

Bruker AVANCE III HD - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 300 uM [U-100% 13C; U-100% 15N] SDE2-DNA Binding protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	SDE2-DNA Binding protein	[U-100% 13C; U-100% 15N]		300 uM
2	Na/K phosphate	natural abundance		50 mM
3	NaCl	natural abundance		50 mM
4	DTT	natural abundance		5 mM

7 3D C(CO)NH

Instrument

Bruker AVANCE III HD - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 300 uM [U-100% 13C; U-100% 15N] SDE2-DNA Binding protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	SDE2-DNA Binding protein	[U-100% 13C; U-100% 15N]		300 uM
2	Na/K phosphate	natural abundance		50 mM
3	NaCl	natural abundance		50 mM
4	DTT	natural abundance		5 mM

8 3D 1H-15N TOCSY

Instrument

Bruker AVANCE III HD - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 300 uM [U-100% 13C; U-100% 15N] SDE2-DNA Binding protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	SDE2-DNA Binding protein	[U-100% 13C; U-100% 15N]		300 uM
2	Na/K phosphate	natural abundance		50 mM
3	NaCl	natural abundance		50 mM
4	DTT	natural abundance		5 mM

9 2D 1H-13C HSQC

Instrument

Bruker AVANCE III HD - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 300 uM [U-100% 13C; U-100% 15N] SDE2-DNA Binding protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	SDE2-DNA Binding protein	[U-100% 13C; U-100% 15N]		300 uM
2	Na/K phosphate	natural abundance		50 mM
3	NaCl	natural abundance		50 mM
4	DTT	natural abundance		5 mM

10 2D 1H-15N HSQC

Instrument

Bruker AVANCE III HD - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 300 uM [U-100% 13C; U-100% 15N] SDE2-DNA Binding protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	SDE2-DNA Binding protein	[U-100% 13C; U-100% 15N]		300 uM
2	Na/K phosphate	natural abundance		50 mM
3	NaCl	natural abundance		50 mM
4	DTT	natural abundance		5 mM

11 3D 1H-15N NOESY

Instrument

Bruker AVANCE III HD - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 300 uM [U-100% 13C; U-100% 15N] SDE2-DNA Binding protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	SDE2-DNA Binding protein	[U-100% 13C; U-100% 15N]		300 uM
2	Na/K phosphate	natural abundance		50 mM
3	NaCl	natural abundance		50 mM
4	DTT	natural abundance		5 mM

12 3D 1H-13C NOESY

Instrument

Bruker AVANCE III HD - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 300 uM [U-100% 13C; U-100% 15N] SDE2-DNA Binding protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	SDE2-DNA Binding protein	[U-100% 13C; U-100% 15N]		300 uM
2	Na/K phosphate	natural abundance		50 mM
3	NaCl	natural abundance		50 mM
4	DTT	natural abundance		5 mM

nullKeywords DNA BINDING PROTEIN, Replication stress response regulator, SAP Domain, SDE2