BMRBj - BMREntryMaster

Found 1 Document (3.025 seconds)

BMRB: 30936

7RIK

Atomic-Resolution Structure of Kinesin-1 Motor Domain in Complex with Polymeric Microtubules by Magic Angle Spinning NMR

Authors

Zhang, C., Guo, C., Russell, R.W., Quinn, C.M., Li, M., Williams, J.C., Gronenborn, A.M., Polenova, T.

Assembly

Kinesin-1 heavy chain

Entity

1. Kinesin-1 heavy chain (polymer), 349 monomers, 39265.97 Da Detail

MADLAECNIK VMCRFRPLNE SEVNRGDKYI AKFQGEDTVV IASKPYAFDR VFQSSTSQEQ VYNDCAKKIV KDVLEGYNGT IFAYGQTSSG KTHTMEGKLH DPEGMGIIPR IVQDIFNYIY SMDENLEFHI KVSYFEIYLD KIRDLLDVSK TNLSVHEDKN RVPYVKGCTE RFVCSPDEVM DTIDEGKSNR HVAVTNMNEH SSRSHSIFLI NVKQENTQTE QKLSGKLYLV DLAGSEKVSK TGAEGAVLDE AKNINKSLSA LGNVISALAE GSTYVPYRDS KMTRILQDSL GGNCRTTIVI CCSPSSYNES ETKSTLLFGQ RAKTIKNTVC VNVELTAEQW KKKYEKEKE

Formula weight

39265.97 Da

Source organism

Homo sapiens

Exptl. method

solid-state NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 85.1 %, Completeness: 68.0 %, Completeness (bb): 79.8 % Detail

Polymer type: polypeptide(L)

	Total	¹³C	¹⁵N
All	68.0 % (1330 of 1957)	67.1 % (1052 of 1568)	71.5 % (278 of 389)
Backbone	79.8 % (1091 of 1368)	79.3 % (814 of 1027)	81.2 % (277 of 341)
Sidechain	54.7 % (502 of 918)	57.6 % (501 of 870)	2.1 % (1 of 48)
Aromatic	33.3 % (45 of 135)	32.8 % (44 of 134)	100.0 % (1 of 1)
Methyl	64.9 % (120 of 185)	64.9 % (120 of 185)

1. entity 1

Show sample condition

Sample #1

Solvent system 100% H2O, Pressure 1 atm, Temperature 283 K, pH 7.4, Details 9.1 % w/w [U-100% 13C; U-100% 15N] Apo-Kif5b, 16.4 % w/w Porcine-alpha-tubulin, 16.4 % w/w Porcine-beta-tubulin, 5 uM TAXOL, 1 mM GUANOSINE-5'-TRIPHOSPHATE, 1 mM GUANOSINE-5'-DIPHOSPHATE, 58.1 % w/w Buffer, 100% H2O

#	Name	Isotope labeling	Concentration
1	Apo-Kif5b	[U-100% 13C; U-100% 15N]	9.1 % w/w
2	Porcine-alpha-tubulin	natural abundance	16.4 % w/w
3	Porcine-beta-tubulin	natural abundance	16.4 % w/w
4	TAXOL	natural abundance	5 uM
5	GUANOSINE-5'-TRIPHOSPHATE	natural abundance	1 mM
6	GUANOSINE-5'-DIPHOSPHATE	natural abundance	1 mM
7	Buffer	natural abundance	58.1 % w/w

Sample #2

#	Name	Isotope labeling	Concentration
8	Apo-Kif5b	[U-100% 13C; U-100% 15N]	9.1 % w/w
9	Porcine-alpha-tubulin	natural abundance	16.4 % w/w
10	Porcine-beta-tubulin	natural abundance	16.4 % w/w
11	TAXOL	natural abundance	5 uM
12	GUANOSINE-5'-TRIPHOSPHATE	natural abundance	1 mM
13	GUANOSINE-5'-DIPHOSPHATE	natural abundance	1 mM
14	Buffer	natural abundance	58.1 % w/w

Sample #3

Solvent system 100% H2O, Pressure 1 atm, Temperature 283 K, pH 7.4, Details 8.2 % w/w [1,6-13C-glucose; U-100% 15N] Apo-Kif5b, 16.4 % w/w Porcine-alpha-tubulin, 16.4 % w/w Porcine-beta-tubulin, 5 uM TAXOL, 1 mM GUANOSINE-5'-TRIPHOSPHATE, 1 mM GUANOSINE-5'-DIPHOSPHATE, 59 % w/w Buffer, 100% H2O

#	Name	Isotope labeling	Concentration
15	Apo-Kif5b	[1,6-13C-glucose; U-100% 15N]	8.2 % w/w
16	Porcine-alpha-tubulin	natural abundance	16.4 % w/w
17	Porcine-beta-tubulin	natural abundance	16.4 % w/w
18	TAXOL	natural abundance	5 uM
19	GUANOSINE-5'-TRIPHOSPHATE	natural abundance	1 mM
20	GUANOSINE-5'-DIPHOSPHATE	natural abundance	1 mM
21	Buffer	natural abundance	59 % w/w

Sample #4

Solvent system 100% H2O, Pressure 1 atm, Temperature 283 K, pH 7.4, Details 7.6 % w/w [2-13C-glucose; U-100% 15N] Apo-Kif5b, 16.4 % w/w Porcine-alpha-tubulin, 16.4 % w/w Porcine-beta-tubulin, 5 uM TAXOL, 1 mM GUANOSINE-5'-TRIPHOSPHATE, 1 mM GUANOSINE-5'-DIPHOSPHATE, 59.6 % w/w Buffer, 100% H2O

#	Name	Isotope labeling	Concentration
22	Apo-Kif5b	[2-13C-glucose; U-100% 15N]	7.6 % w/w
23	Porcine-alpha-tubulin	natural abundance	16.4 % w/w
24	Porcine-beta-tubulin	natural abundance	16.4 % w/w
25	TAXOL	natural abundance	5 uM
26	GUANOSINE-5'-TRIPHOSPHATE	natural abundance	1 mM
27	GUANOSINE-5'-DIPHOSPHATE	natural abundance	1 mM
28	Buffer	natural abundance	59.6 % w/w

Release date

2021-08-04

Citation

Magic-angle-spinning NMR structure of the kinesin-1 motor domain assembled with microtubules reveals the elusive neck linker orientation
Zhang, C., Guo, C., Russell, R.W., Quinn, C.M., Li, M., Williams, J.C., Gronenborn, A.M., Polenova, T.
Nat. Commun. (2022), 13, 6795-6795, PubMed 36357375 , DOI 10.1038/s41467-022-34026-w , Abstract

Related entities 1. Kinesin-1 heavy chain, : 1 : 3 : 18 : 274 entities Detail

Interaction partners 1. Kinesin-1 heavy chain, : 68 interactors Detail

More details for 68 interactors identified by 35 citations

Experiments performed 7 experiments Detail

1 2D 13C-detected CORD

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system 100% H2O, Pressure 1 atm, Temperature 283 K, pH 7.4, Details 9.1 % w/w [U-100% 13C; U-100% 15N] Apo-Kif5b, 16.4 % w/w Porcine-alpha-tubulin, 16.4 % w/w Porcine-beta-tubulin, 5 uM TAXOL, 1 mM GUANOSINE-5'-TRIPHOSPHATE, 1 mM GUANOSINE-5'-DIPHOSPHATE, 58.1 % w/w Buffer, 100% H2O

#	Name	Isotope labeling	Concentration
1	Apo-Kif5b	[U-100% 13C; U-100% 15N]	9.1 % w/w
2	Porcine-alpha-tubulin	natural abundance	16.4 % w/w
3	Porcine-beta-tubulin	natural abundance	16.4 % w/w
4	TAXOL	natural abundance	5 uM
5	GUANOSINE-5'-TRIPHOSPHATE	natural abundance	1 mM
6	GUANOSINE-5'-DIPHOSPHATE	natural abundance	1 mM
7	Buffer	natural abundance	58.1 % w/w

2 2D 13C-detected CORD

Instrument

Bruker AVANCE NEO - 600 MHz CryoProbe

Sample

#	Name	Isotope labeling	Concentration
8	Apo-Kif5b	[U-100% 13C; U-100% 15N]	9.1 % w/w
9	Porcine-alpha-tubulin	natural abundance	16.4 % w/w
10	Porcine-beta-tubulin	natural abundance	16.4 % w/w
11	TAXOL	natural abundance	5 uM
12	GUANOSINE-5'-TRIPHOSPHATE	natural abundance	1 mM
13	GUANOSINE-5'-DIPHOSPHATE	natural abundance	1 mM
14	Buffer	natural abundance	58.1 % w/w

3 2D 13C-detected CORD

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system 100% H2O, Pressure 1 atm, Temperature 283 K, pH 7.4, Details 8.2 % w/w [1,6-13C-glucose; U-100% 15N] Apo-Kif5b, 16.4 % w/w Porcine-alpha-tubulin, 16.4 % w/w Porcine-beta-tubulin, 5 uM TAXOL, 1 mM GUANOSINE-5'-TRIPHOSPHATE, 1 mM GUANOSINE-5'-DIPHOSPHATE, 59 % w/w Buffer, 100% H2O

#	Name	Isotope labeling	Concentration
15	Apo-Kif5b	[1,6-13C-glucose; U-100% 15N]	8.2 % w/w
16	Porcine-alpha-tubulin	natural abundance	16.4 % w/w
17	Porcine-beta-tubulin	natural abundance	16.4 % w/w
18	TAXOL	natural abundance	5 uM
19	GUANOSINE-5'-TRIPHOSPHATE	natural abundance	1 mM
20	GUANOSINE-5'-DIPHOSPHATE	natural abundance	1 mM
21	Buffer	natural abundance	59 % w/w

4 2D 13C-detected CORD

Instrument

Bruker AVANCE NEO - 600 MHz CryoProbe

Sample

State isotropic, Solvent system 100% H2O, Pressure 1 atm, Temperature 283 K, pH 7.4, Details 7.6 % w/w [2-13C-glucose; U-100% 15N] Apo-Kif5b, 16.4 % w/w Porcine-alpha-tubulin, 16.4 % w/w Porcine-beta-tubulin, 5 uM TAXOL, 1 mM GUANOSINE-5'-TRIPHOSPHATE, 1 mM GUANOSINE-5'-DIPHOSPHATE, 59.6 % w/w Buffer, 100% H2O

#	Name	Isotope labeling	Concentration
22	Apo-Kif5b	[2-13C-glucose; U-100% 15N]	7.6 % w/w
23	Porcine-alpha-tubulin	natural abundance	16.4 % w/w
24	Porcine-beta-tubulin	natural abundance	16.4 % w/w
25	TAXOL	natural abundance	5 uM
26	GUANOSINE-5'-TRIPHOSPHATE	natural abundance	1 mM
27	GUANOSINE-5'-DIPHOSPHATE	natural abundance	1 mM
28	Buffer	natural abundance	59.6 % w/w

5 2D NCACX

Instrument

Bruker AVANCE NEO - 600 MHz CryoProbe

Sample

#	Name	Isotope labeling	Concentration
8	Apo-Kif5b	[U-100% 13C; U-100% 15N]	9.1 % w/w
9	Porcine-alpha-tubulin	natural abundance	16.4 % w/w
10	Porcine-beta-tubulin	natural abundance	16.4 % w/w
11	TAXOL	natural abundance	5 uM
12	GUANOSINE-5'-TRIPHOSPHATE	natural abundance	1 mM
13	GUANOSINE-5'-DIPHOSPHATE	natural abundance	1 mM
14	Buffer	natural abundance	58.1 % w/w

6 3D NCOCX

Instrument

Bruker AVANCE NEO - 600 MHz CryoProbe

Sample

#	Name	Isotope labeling	Concentration
8	Apo-Kif5b	[U-100% 13C; U-100% 15N]	9.1 % w/w
9	Porcine-alpha-tubulin	natural abundance	16.4 % w/w
10	Porcine-beta-tubulin	natural abundance	16.4 % w/w
11	TAXOL	natural abundance	5 uM
12	GUANOSINE-5'-TRIPHOSPHATE	natural abundance	1 mM
13	GUANOSINE-5'-DIPHOSPHATE	natural abundance	1 mM
14	Buffer	natural abundance	58.1 % w/w

7 3D NCACX

Instrument

Bruker AVANCE NEO - 600 MHz CryoProbe

Sample

#	Name	Isotope labeling	Concentration
8	Apo-Kif5b	[U-100% 13C; U-100% 15N]	9.1 % w/w
9	Porcine-alpha-tubulin	natural abundance	16.4 % w/w
10	Porcine-beta-tubulin	natural abundance	16.4 % w/w
11	TAXOL	natural abundance	5 uM
12	GUANOSINE-5'-TRIPHOSPHATE	natural abundance	1 mM
13	GUANOSINE-5'-DIPHOSPHATE	natural abundance	1 mM
14	Buffer	natural abundance	58.1 % w/w

nullKeywords Kif5b, MOTOR PROTEIN, MTs, integrated structural biology, kinesin-1 motor domain, microtubules

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Found 1 Document (3.025 seconds)

BMRB: 30936

Sample #1

Sample #2

Sample #3

Sample #4

Related entities 1. Kinesin-1 heavy chain, : 1 : 3 : 18 : 274 entities Detail

Interaction partners 1. Kinesin-1 heavy chain, : 68 interactors Detail

Experiments performed 7 experiments Detail

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